AAA+ ATPase chaperone p97/VCPFAF2 governs basal pexophagy [PDF]
Nature CommunicationsPeroxisomes are organelles that are central to lipid metabolism and chemical detoxification. Despite advances in our understanding of peroxisome biogenesis, the mechanisms maintaining peroxisomal membrane proteins remain to be fully elucidated.
Fumika Koyano +6 more
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Visualization of the Cdc48 AAA+ ATPase protein unfolding pathway [PDF]
Nature CommunicationsThe Cdc48 AAA+ ATPase is an abundant and essential enzyme that unfolds substrates in multiple protein quality control pathways. The enzyme includes two conserved AAA+ ATPase motor domains, D1 and D2, that assemble as hexameric rings with D1 stacked above
Ian Cooney +7 more
doaj +4 more sources
The ATPase Activity of Escherichia coli Expressed AAA+-ATPase Protein [PDF]
Bio-Protocol, 2020 ATPases are the enzymes that breakdown ATP to ADP and release inorganic phosphate (Pi). Here we provide a detailed protocol to determine the ATPase activity of a recombinant AAA+-ATPase protein (GENERAL CONTROL NON-REPRESSIBLE-4 [GCN4]) by ...
Amita Kaundal +2 more
doaj +4 more sources
AAA-ATPases in Protein Degradation [PDF]
Frontiers in Molecular Biosciences, 2017 Proteolytic machineries containing multisubunit protease complexes and AAA-ATPases play a key role in protein quality control and the regulation of protein homeostasis. In these protein degradation machineries, the proteolytically active sites are formed
Ravikiran S. Yedidi +2 more
doaj +3 more sources
Bromodomain AAA+ ATPases get into shape [PDF]
Nucleus, 2020 Bromodomain AAA+ ATPases (ATPases associated with diverse cellular activities) are emerging as oncogenic proteins and compelling targets for anticancer therapies. However, structural and biochemical insight into these machines is missing.
Magdalena Murawska, Andreas G. Ladurner
doaj +3 more sources
The Multivesicular Bodies (MVBs)-Localized AAA ATPase LRD6-6 Inhibits Immunity and Cell Death Likely through Regulating MVBs-Mediated Vesicular Trafficking in Rice. [PDF]
PLoS Genetics, 2016 Previous studies have shown that multivesicular bodies (MVBs)/endosomes-mediated vesicular trafficking may play key roles in plant immunity and cell death. However, the molecular regulation is poorly understood in rice.
Xiaobo Zhu +22 more
doaj +2 more sources
A novel AAA+ ATPase required for sporulation and stress response in Bacillus anthracis [PDF]
Journal of BacteriologyAAA+ proteins function as molecular machines that utilize ATP to perform diverse cellular functions, including protein homeostasis, stress regulation, and cell cycle/developmental processes.
Nitika Sangwan +12 more
doaj +2 more sources
AAA+ ATPases in Protein Degradation: Structures, Functions and Mechanisms [PDF]
Biomolecules, 2020 Adenosine triphosphatases (ATPases) associated with a variety of cellular activities (AAA+), the hexameric ring-shaped motor complexes located in all ATP-driven proteolytic machines, are involved in many cellular processes.
Shuwen Zhang, Youdong Mao
doaj +3 more sources
The AAA+ ATPase p97, a cellular multitool [PDF]
Biochemical Journal, 2017 The AAA+ (ATPases associated with diverse cellular activities) ATPase p97 is essential to a wide range of cellular functions, including endoplasmic reticulum-associated degradation, membrane fusion, NF-κB (nuclear factor kappa-light-chain-enhancer of ...
L. Stach, P. Freemont
semanticscholar +5 more sources
Fragment screening using biolayer interferometry reveals ligands targeting the SHP-motif binding site of the AAA+ ATPase p97 [PDF]
Communications Chemistry, 2022 The AAA+ ATPase p97 protein is thought to be a potential anticancer target, but direct targeting on its ATPase function has not proven to be a successful strategy in clinical trials due to lack of selectivity.
Sebastian Bothe +9 more
doaj +2 more sources