Results 21 to 30 of about 3,365 (168)

Development of p97 AAA ATPase inhibitors [PDF]

Autophagy, 2011
Specific p97 inhibitors are valuable research tools to carry out mechanistic and cellular investigations of p97 biology. p97 is an abundant, ubiquitin-selective chaperone that has multiple functions and is essential for life. Therefore, genetic methods that require long incubations like siRNA or expression of dominant-negative p97 mutants are likely to
Chou, Tsui-Fen, Deshaies, Raymond J.
openaire   +4 more sources

Torsins: not your typical AAA+ ATPases [PDF]

Critical Reviews in Biochemistry and Molecular Biology, 2015
Torsin ATPases (Torsins) belong to the widespread AAA+ (ATPases associated with a variety of cellular activities) family of ATPases, which share structural similarity but have diverse cellular functions. Torsins are outliers in this family because they lack many characteristics of typical AAA+ proteins, and they are the only members of the AAA+ family ...
April E, Rose, Rebecca S H, Brown, Christian, Schlieker   +2 more
openaire   +2 more sources

The Archaeal Proteasome Is Regulated by a Network of AAA ATPases [PDF]

Journal of Biological Chemistry, 2012
The proteasome is the central machinery for targeted protein degradation in archaea, Actinobacteria, and eukaryotes. In its basic form, it consists of a regulatory ATPase complex and a proteolytic core particle. The interaction between the two is governed by an HbYX motif (where Hb is a hydrophobic residue, Y is tyrosine, and X is any amino acid) at ...
Forouzan, D., Ammelburg, M., Hobel, C., Ströh, L., Sessler, N., Martin, J., Lupas, A.   +6 more
openaire   +3 more sources

Bacterial Enhancer Binding Proteins—AAA⁺ Proteins in Transcription Activation

Biomolecules, 2020
Bacterial enhancer-binding proteins (bEBPs) are specialised transcriptional activators. bEBPs are hexameric AAA+ ATPases and use ATPase activities to remodel RNA polymerase (RNAP) complexes that contain the major variant sigma factor, σ54 to convert
Forson Gao, Amy E. Danson, Fuzhou Ye, Milija Jovanovic, Martin Buck, Xiaodong Zhang   +5 more
doaj   +1 more source

Fragment screening using biolayer interferometry reveals ligands targeting the SHP-motif binding site of the AAA+ ATPase p97

Communications Chemistry, 2022
The AAA+ ATPase p97 protein is thought to be a potential anticancer target, but direct targeting on its ATPase function has not proven to be a successful strategy in clinical trials due to lack of selectivity.
Sebastian Bothe, Petra Hänzelmann, Stephan Böhler, Josef Kehrein, Markus Zehe, Christoph Wiedemann, Ute A. Hellmich, Ruth Brenk, Hermann Schindelin, Christoph Sotriffer   +9 more
doaj   +1 more source

The proteasome cap RPT5/Rpt5p subunit prevents aggregation of unfolded ricin A chain [PDF]

, 2013
The plant cytotoxin ricin enters mammalian cells by receptor-mediated endocytosis, undergoing retrograde transport to the endoplasmic reticulum (ER) where its catalytic A chain (RTA) is reductively separated from the holotoxin to enter the cytosol and ...
Spooner, Robert A., Roberts, David M, Roberts, Lynne M, Lord, J Michael, Cook, Jonathan P., Pietroni, Paola, Roberts, David M., Lord, Mike, Roberts, L. M. (Lynne M.), Spooner, Robert A, Vasisht, Nishi, Hartmann-Petersen, Rasmus, Cook, Jonathan P   +12 more
core   +1 more source

Structure and function of the AAA+ ATPase p97/Cdc48p [PDF]

Gene, 2016
p97 (also known as valosin-containing protein (VCP) in mammals or Cdc48p in Saccharomyces cerevisiae) is an evolutionarily conserved ATPase present in all eukaryotes and archaebacteria. In conjunction with a collection of cofactors and adaptors, p97/Cdc48p performs an array of biological functions mostly through modulating the stability of 'client ...
Di, Xia, Wai Kwan, Tang, Yihong, Ye
openaire   +2 more sources

Structural dynamics of AAA + ATPase Drg1 and mechanism of benzo-diazaborine inhibition

Nature Communications, 2022
Drg1 is a cytoplasmic AAA + ATPase responsible for releasing Rlp24 during ribosome assembly. Here, the authors show that Drg1 structurally and functionally shares many regulatory features with that of Cdc48/p97, suggesting a unfoldase role for Drg1.
Chengying Ma, Damu Wu, Qian Chen, Ning Gao   +3 more
doaj   +1 more source

The Oligomeric State of the Active Vps4 AAA ATPase [PDF]

Journal of Molecular Biology, 2014
The cellular ESCRT (endosomal sorting complexes required for transport) pathway drives membrane constriction toward the cytosol and effects membrane fission during cytokinesis, endosomal sorting, and the release of many enveloped viruses, including the human immunodeficiency virus.
Monroe Nicole, Han Han, Gonciarz Malgorzata D, Eckert Debra M, Karren Mary Anne, Whitby Frank G, Sundquist Wesley I, Hill Christopher P   +7 more
openaire   +3 more sources

A conserved inter-domain communication mechanism regulates the ATPase activity of the AAA-protein Drg1

, 2017
Contains fulltext : 169936.pdf (Publisher’s version ) (Open Access)AAA-ATPases fulfil essential roles in different cellular pathways and often act in form of hexameric complexes.
Elmar Krieger, Karl Gruber, Daniel Luschnig, Ingrid Rössler, Manuela Grassegger, Michael Prattes, Lisa Kappel, Helmut Bergler, Altijana Hromic, Brigitte Pertschy, Mathias Loibl, Gertrude Zisser   +11 more
core   +2 more sources