Results 21 to 30 of about 89,643 (298)

Generation of enhanced gene delivery vectors by directed evolution of adeno-associated virus [PDF]

, 2006
Despite promising advance in the development of viral vectors based on AAV for human gene therapy, several major hurdles for a more general use remain.
Endell, Jan
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A novel dual mechanism-of-action bispecific PD-1-IL-2v armed by a “βγ-only” interleukin-2 variant

Frontiers in Immunology
IntroductionInterleukin-2 (IL-2) is one of the first cytokines to be discovered as an immune agonist for cancer immunotherapy. Biased IL-2 variants had been discovered to eliminate Treg activation or enhance the tumor specific T cell cytotoxicity ...
Yongji Jiang, Chuyuan Chen, Yuan Liu, Rong Wang, Chuan Feng, Lili Cai, Shuang Chang, Lei Zhao   +7 more
doaj   +1 more source

Characterization of Brevibacillus laterosporus Cas9 (BlatCas9) for Mammalian Genome Editing

Frontiers in Cell and Developmental Biology, 2020
Compact CRISPR/Cas9 systems that can be delivered by AAV for in vivo genome editing hold great promise for clinical applications. Brevibacillus laterosporus Cas9 (BlatCas9) is a compact Cas9 nuclease that has been identified for plant genome editing ...
Ning Gao, Ning Gao, Chengdong Zhang, Ziying Hu, Miaomiao Li, Jingjing Wei, Yongming Wang, Yongming Wang, Huihui Liu   +8 more
doaj   +1 more source

PEP-TP56 enhances AAV transduction in a serotype-independent manner

Heliyon
Adeno-associated viruses (AAVs) have emerged as the most prominent gene therapy vectors. However, the need for high AAV doses to achieve clinical efficacy remains a key challenge to AAV utility in the clinic.
Olaniyi Olarewaju, Franziska Held, Christian Schoen, Udo Maier, Francois du Plessis   +4 more
doaj   +1 more source

AAV-Tis21 treatment decreases Cyclin D1 and Cyclin E and increases NeuroD1 mRNA expression levels in MB allograft tissues.

, 2018
Real-time PCR analysis of RNA obtained from the nodules treated with AAV overexpressing Tis21 (AAV-Tis21) or AAV-CBA (control). Three nodules per group were analyzed. Average ± SEM values are from (at least) three independent experiments and are shown as
Manuela Ceccarelli (4398151), Giuliana Papoff (688457), Simone Samperna (828290), Felice Tirone (256018), Simonetta Santini (828289), Laura Micheli (256012), Cristiana Lalli (687846), Lorena Zentilin (1933051), Dario Presutti (828288), Giovina Ruberti (687850)   +9 more
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Adeno-Associated Virus (AAV) - Based Gene Therapies for Retinal Diseases: Where are We?

The Application of Clinical Genetics, 2023
Divya Ail, Hugo Malki, Emilia A Zin, Deniz Dalkara Sorbonne Université, INSERM, CNRS, Department of Therapeutics, Institut de la Vision, Paris, 75012, FranceCorrespondence: Divya Ail, Department of Therapeutics, Institut de la Vision, 17 Rue Moreau ...
Ail D, Malki H, Zin EA, Dalkara D
doaj  

AAV-mediated overexpression of Cytl1 induces CF.

, 2016
Control virus or AAV-Cytl1 (5 × 1010 viral genome) was injected into the tail vein of WT mice, and the phenotype of the heart was examined after 8 wks. (A) Picrosirius staining of heart cross-sections from WT mice injected with control virus or AAV-Cytl.
Roger J. Hajjar (142214), Dongtak Jeong (142212), Woo Jin Park (142218), Jooyeon Kim (3343688), Seung Hee Lee (1828798), Jihwa Kim (715350), Sacha V. Kepreotis (3343685), Jimeen Yoo (3343682), Jang-Soo Chun (575973)   +8 more
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Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors [PDF]

, 2004
BACKGROUND: Inactivating genes in vivo is an important technique for establishing their function in the adult nervous system. Unfortunately, conventional knockout mice may suffer from several limitations including embryonic or perinatal lethality and the
Hermens, Wim T J M C, Lieberman, A., Eggers, R., Verhaagen, J., Sablitzky, Fred, Chakravarthy, Sridhara, Zhang, Jing Ying, Zhang, J.Y., Hermens, Wim TJMC, Zhang, Jing, Verhaagen, J, Ahmed, Bushra, Anderson, P.N., Eggers, R, Eggers, Ruben, Levelt, Christiaan, Chakravarthy, S, Levelt, C., Ahmed, BY, Verhaagen, Joost, Lieberman, A.R., Hermens, WTJMC, Lieberman, AR, Anderson, Patrick N., Ahmed, Bushra Y, Hermens, W.T., Niclou, SP, Anderson, PN, NICLOU, Simone P., Lieberman, A R, Zhang, JY, Anderson, Patrick N, Sablitzky, F., Levelt, C, Sablitzky, F, Niclou, S.P., Chakravarthy, S., Ahmed, B.Y.   +37 more
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Thorough molecular configuration analysis of noncanonical AAV genomes in AAV vector preparations [PDF]

The unique palindromic inverted terminal repeats (ITRs) and single-stranded nature of adeno-associated virus (AAV) DNA are major hurdles to current sequencing technologies.
Roland W. Herzog, Matthew Chrzanowski, Tian, Jiahe, Chrzanowski, Matthew, Zhang, Junping, Yu, Xiangping, Jiahe Tian, Junping Zhang, Ping Guo, Herzog, Roland W., Derek Pouchnik, Guo, Ping, Xiao, Weidong, Weidong Xiao, Xiangping Yu, Pouchnik, Derek   +15 more
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Targeted genetic manipulations of neuronal subtypes using promoter-specific combinatorial AAVs for use in wild-type animals

Frontiers in Behavioral Neuroscience, 2015
Techniques to genetically manipulate the activity of defined neuronal subpopulations have been useful in elucidating function, however applicability to translational research beyond transgenic mice is limited. Subtype targeted transgene expression can be
Heinrich S Gompf, Evgeny A Budygin, Evgeny A Budygin, Patrick M Fuller, Caroline E Bass   +4 more
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