Results 51 to 60 of about 26,423 (304)

Identification of actins in foraminifera: Phylogenetic perspectives

, 1995
Actin was identified by immunoblotting with two monoclonal antibodies in 13 species of foraminifera representing four major taxonomic groups (Allogromiina, Textulariina, Rotaliina and Miliolina).
Jan Pawlowski, Fahrni, José, Pawlowski, Jan Wojciech, José F. Fahrni   +3 more
core   +1 more source

The mother of all actins?

eLife, 2016
New insights into the structure of filaments made of crenactin, a homolog of actin found in archaea, shed light on how the cytoskeleton might have evolved.
Felipe Merino, Stefan Raunser
doaj   +1 more source

A novel microfluidic flow-cytometry for counting numbers of single-cell β-actins

Nanotechnology and Precision Engineering, 2020
As a house keeping protein with stable expressions, β-actin is used as a loading control in normalization of western blotting. However, the actual numbers of β-actins at the single-cell level remain elusive.
Lixing Liu, Beiyuan Fan, Hongyu Yang, Deyong Chen, Shuang Zhang, Junbo Wang, Jian Chen   +6 more
doaj   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

TNIK Regulates Cytoskeletal Organization to Promote Focal Adhesion Turnover and Mitosis in Lung Adenocarcinoma

Frontiers in Bioscience-Landmark
Background: Lung cancer is the primary cause of cancer-related mortality, but the molecular mechanisms behind this malignancy remain unclear.
Yao Li, Meng-yao Song, Xing Hu, Xue-hua Sun, Tao Zhang, Lu Zhang, Ying-xiong Wang, Qian Zhang, Chun-dong Zhang, Lian Zhang   +9 more
doaj   +1 more source

A method for rapidly screening functionality of actin mutants and tagged actins [PDF]

Biological Procedures Online, 2004
Recombinant production and biochemical analysis of actin mutants has been hampered by the fact that actin has an absolute requirement for the eukaryotic chaperone CCT to reach its native state.
Rommelaere Heidi, Waterschoot Davy, Neirynck Katrien, Vandekerckhove Joël, Ampe Christophe   +4 more
doaj   +1 more source

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source

Partially Redundant Actin Genes in Chlamydomonas Control Transition Zone Organization and Flagellum-Directed Traffic

Cell Reports, 2019
Summary: The unicellular green alga Chlamydomonas reinhardtii is a biflagellated cell with two actin genes: one encoding a conventional actin (IDA5) and the other encoding a divergent novel actin-like protein (NAP1).
Brittany Jack, David M. Mueller, Ann C. Fee, Ashley L. Tetlow, Prachee Avasthi   +4 more
doaj   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Divergent Plasmodium actin residues are essential for filament localization, mosquito salivary gland invasion and malaria transmission.

PLoS Pathogens, 2022
Actin is one of the most conserved and ubiquitous proteins in eukaryotes. Its sequence has been highly conserved for its monomers to self-assemble into filaments that mediate essential cell functions such as trafficking, cell shape and motility.
Michelle Yee, Tobias Walther, Friedrich Frischknecht, Ross G Douglas   +3 more
doaj   +1 more source