Results 101 to 110 of about 982,671 (310)

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Mengoptimalkan Pendampingan Berbasis KKG dalam Upaya Meningkatkan Kompetensi Guru Kelas SD 29 Mataram dalam Penyusunan RPP

Jurnal Kependidikan, 2017
This study aims to find out how far the effectiveness of the implementation of assistance in improving the competence of teachers SD Negeri 29 Mataram in the preparation of Learning Implementation Plan (RPP) based on the character education of the nation
Agus Salim
doaj   +1 more source

IMAGACT: Deriving an Action Ontology from Spoken Corpora [PDF]

, 2012
This paper presents the IMAGACT annotation infrastructure which uses both corpus - based and competence - based methods for the simultaneous extraction of a language independent Action ontology from English and Italian spontaneous speech corpora.
Frontini, Francesca, Gagliardi, Gloria, Monachini, Monica, Moneglia, Massimo, Panunzi, Alessandro, Russo, Irene   +5 more
core   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

‘Facultative’ and ‘Functional Mixity’ in light of the Principle of Partial and Imperfect Conferral. College of Europe Research Paper in Law 03/2019 [PDF]

, 2019
The concept of ‘facultative mixity’ as first coined by Allan Rosas3 has sparked a much heated debate.4 Is it a matter of political expediency in the EU Council to decide on the mixed nature, or not, of a given agreement in so far as it falls within ...
Govaere, Inge
core  

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

The evolution of human resources from crude oil extraction and processing activities in Romania between 1990 and 2006 [PDF]

Providing qualified workers for the companies represents a step forward the normal and efficient operation of them. The modern industrial company presupposes that people should interact to each other, establishing multiple connections and passing through
Mirela Ciobanu
core  

The role of effective communication and trustworthiness in determining guests’ loyalty [PDF]

, 2018
The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link.This study investigates the role of trustworthiness and effective communication in the development of ...
Fazal-e-Hasan, Syed Muhammad, Hickman, Ellie, Kharouf, Husni, Mortimer, Gary, Sekhon, Harjit   +4 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source