Results 41 to 50 of about 47,958 (242)

Gating mechanism of elongating β-ketoacyl-ACP synthases

Nature Communications, 2020
The formation of C-C bonds in fatty acid and polyketide biosynthesis depends on β-ketoacyl-acyl carrier protein (ACP) synthases (KSs). Here, the authors present structures of E.coli KSs bound to substrate mimetic bearing ACPs, providing insights into the
Jeffrey T. Mindrebo, Ashay Patel, Woojoo E. Kim, Tony D. Davis, Aochiu Chen, Thomas G. Bartholow, James J. La Clair, J. Andrew McCammon, Joseph P. Noel, Michael D. Burkart   +9 more
doaj   +1 more source

Subtractive genomics and molecular docking approach to identify drug targets against Stenotrophomonas maltophilia.

PLoS ONE, 2021
Stenotrophomonas maltophilia is a multidrug resistant pathogen associated with high mortality and morbidity in patients having compromised immunity. The efflux systems of S.
Hira Saleem, Usman Ali Ashfaq, Habibullah Nadeem, Muhammad Zubair, Muhammad Hussnain Siddique, Ijaz Rasul   +5 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Sertraline Treatment Can Mimic Niemann‐Pick Type C Biomarker Profile: A Diagnostic Pitfall

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background Oxysterols (cholestane‐3β,5α,6β‐triol and 7‐ketocholesterol) and N‐palmitoyl‐O‐phosphocholineserine (PPCS) are sensitive biomarkers for Niemann‐Pick disease type C (NPC) screening. However, false‐positive results occur, with a biomarker profile suggestive of NPC despite the absence of pathogenic variants in genes involved in NPC or ...
Maria Makrygianni, Cecile Pagan, Antony Citterio‐Quentin, Isabelle Rouvet, Giulia Dingeo, Cyril Hanin, Claudine Laurent‐Levinson, Samia Pichard, Bénédicte Héron, Clément Gourguechon, Daniele Mandia, Jerome Guitton, Carole Lacout, Nicolas Schleinitz, Florient Potier, Olivier Flabeau, Elsa Besse‐Pinot, Foudil Lamari, Magali Pettazzoni, Yann Nadjar   +19 more
wiley   +1 more source

CoA‐dependent activation of mitochondrial acyl carrier protein links four neurodegenerative diseases

EMBO Molecular Medicine, 2019
PKAN, CoPAN, MePAN, and PDH‐E2 deficiency share key phenotypic features but harbor defects in distinct metabolic processes. Selective damage to the globus pallidus occurs in these genetic neurodegenerative diseases, which arise from defects in CoA ...
Roald A Lambrechts, Hein Schepers, Yi Yu, Marianne van der Zwaag, Kaija J Autio, Marcel A Vieira‐Lara, Barbara M Bakker, Marina A Tijssen, Susan J Hayflick, Nicola A Grzeschik, Ody CM Sibon   +10 more
doaj   +1 more source

Gellan Gum Fluid Gel System for Controlled‐Delivery of Cytokine‐Licensed MSC‐EVs to Enhance Corneal Repair in Limbal Stem Cell Deficiency

Advanced Healthcare Materials, EarlyView.
Gellan gum fluid gel (FG) enables controlled ocular delivery of mesenchymal stromal cell (MSC)‐derived extracellular vesicles (EV). Its shear‐thinning, solid‐liquid‐solid rheology ensures prolonged ocular retention. Sustained MSC‐EV release (∼18% in 6 hrs) supports effective EV ocular delivery.
Seyedmohammad Moosavizadeh, Ava Dashti, Thomas E. Robinson, Robyn Gaffey, Richard Moakes, Neil Lagali, Liam Grover, Thomas Ritter   +7 more
wiley   +1 more source

In Silico Approach to Identify Potent Bioactive Pyrrole Analogs as Inhibitors against the Enoyl-acyl Carrier Protein (ACP) Reductase Enzyme of Mycobacterium tuberculosis

RGUHS Journal of Pharmaceutical Sciences
Background In Mycobacterium tuberculosis the enzyme enoyl-acyl carrier protein ACP reductase InhA catalyzes the elongation of acyl fatty acids which are essential building blocks of the
Tabssum Inamdar, Shrinivas Joshi, Sravanthi Avunoori   +2 more
doaj   +1 more source

Structural stability of Cutibacterium acnes acyl carrier protein studied using CD and NMR spectroscopy

Journal of Analytical Science and Technology, 2022
To survive in diverse environments, bacteria adapt by changing the composition of their cell membrane fatty acids. Compared with aerobic bacteria, Cutibacterium acnes has much greater contents of branched-chain fatty acids (BCFAs) in the cell membrane ...
Ahjin Jang, Dasom Cheon, Eunha Hwang, Yangmee Kim   +3 more
doaj   +1 more source

Engineering Nanoplatforms for Alzheimer's Disease Detection via Biomolecular Corona Proteomic and Lipidomic Profiling

Advanced Healthcare Materials, EarlyView.
Engineered nanoparticles capture disease‐specific biomolecular coronas that uncover hidden molecular features of Alzheimer's disease. Combined proteomic and lipidomic analyses reveal a characteristic shift in ribosomal machinery and energy metabolism, generating a multiomic fingerprint that supports accurate disease detection and opens new ...
Antonietta Greco, Roberto Baretta, Andrea Di Fonzo, Andrea Armirotti, Stefano Cinti, Mara Mirasoli, Marco Frasconi, Claudia Corbo   +7 more
wiley   +1 more source

2-Acylglycerolphosphoethanolamine acyltransferase/acyl-acyl carrier protein synthetase is a membrane-associated acyl carrier protein binding protein

Journal of Biological Chemistry, 1989
Two enzymatic activities, 2-acylglycerolphosphoethanolamine (2-acyl-GPE) acyltransferase and acyl-acyl carrier protein (acyl-ACP) synthetase, were solubilized and purified from Escherichia coli membranes. Electrophoretic analysis of the final product of the purification procedure revealed a single protein species with an apparent molecular mass of 27 ...
C L, Cooper, L, Hsu, S, Jackowski, C O, Rock   +3 more
openaire   +2 more sources