Results 21 to 30 of about 17,541 (293)

Alkyne lipids as substrates for click chemistry-based in vitro enzymatic assays

Journal of Lipid Research, 2013
Click chemistry is evolving as a powerful tool in biological applications because it allows the sensitive and specific detection of compounds with alkyne or azido groups.
Anne Gaebler, Robin Milan, Leon Straub, Dominik Hoelper, Lars Kuerschner, Christoph Thiele   +5 more
doaj   +1 more source

The structural basis for the phospholipid remodeling by lysophosphatidylcholine acyltransferase 3

Nature Communications, 2021
During phosphatidylcholine (PC) remodeling re-acylation is catalyzed by lysophosphatidylcholine acyltransferases (LPCAT). Here, the authors present crystal and cryo-EM structures of chicken LPCAT3 in the apo-, acyl donor-bound and acyl receptor-bound ...
Qing Zhang, Deqiang Yao, Bing Rao, Liyan Jian, Yang Chen, Kexin Hu, Ying Xia, Shaobai Li, Yafeng Shen, An Qin, Jie Zhao, Lu Zhou, Ming Lei, Xian-Cheng Jiang, Yu Cao   +14 more
doaj   +1 more source

S-acylation of P2K1 mediates extracellular ATP-induced immune signaling in Arabidopsis

Nature Communications, 2021
S-acylation is a reversible protein post-translational modification that often regulates protein function at the plasma membrane. Here the authors show that an Arabidopsis extracellular ATP receptor P2K1 mediates phosphorylation of two S-acyltransferases
Dongqin Chen, Fengsheng Hao, Huiqi Mu, Nagib Ahsan, Jay J. Thelen, Gary Stacey   +5 more
doaj   +1 more source

Acyl-coenzyme A:cholesterol acyltransferases [PDF]

American Journal of Physiology-Endocrinology and Metabolism, 2009
The enzymes acyl-coenzyme A (CoA):cholesterol acyltransferases (ACATs) are membrane-bound proteins that utilize long-chain fatty acyl-CoA and cholesterol as substrates to form cholesteryl esters. In mammals, two isoenzymes, ACAT1 and ACAT2, encoded by two different genes, exist.
Ta-Yuan, Chang, Bo-Liang, Li, Catherine C Y, Chang, Yasuomi, Urano   +3 more
openaire   +2 more sources

Characterization of two Arabidopsis thaliana acyltransferases with preference for lysophosphatidylethanolamine

BMC Plant Biology, 2009
Background Two previously uncharacterized Arabidopsis genes that encode proteins with acyltransferase PlsC regions were selected for study based on their sequence similarity to a recently identified lung lysophosphatidylcholine acyltransferase (LPCAT ...
Stymne Sten, Ståhl Ulf, Stålberg Kjell, Ohlrogge John   +3 more
doaj   +1 more source

Versatility in acyltransferase activity completes chicoric acid biosynthesis in purple coneflower

Nature Communications, 2021
Biosynthetic pathway of chicoric acid in purple coneflower has not been fully elucidated, though the compound has been shown to have potential health benefits.
Rao Fu, Pingyu Zhang, Ge Jin, Lianglei Wang, Shiqian Qi, Yang Cao, Cathie Martin, Yang Zhang   +7 more
doaj   +1 more source

Rational Design for Enhanced Acyltransferase Activity in Water Catalyzed by the Pyrobaculum calidifontis VA1 Esterase

Microorganisms, 2021
Biocatalytic transesterification is commonly carried out employing lipases in anhydrous organic solvents since hydrolases usually prefer hydrolysis over acyl transfer in bulk water.
Amanda Staudt, Henrik Terholsen, Jasmin Kaur, Henrik Müller, Simon P. Godehard, Ivaldo Itabaiana, Ivana C. R. Leal, Uwe T. Bornscheuer   +7 more
doaj   +1 more source

Identification and Characterization of Sterol Acyltransferases Responsible for Steryl Ester Biosynthesis in Tomato

Frontiers in Plant Science, 2018
Steryl esters (SEs) serve as a storage pool of sterols that helps to maintain proper levels of free sterols (FSs) in cell membranes throughout plant growth and development, and participates in the recycling of FSs and fatty acids released from cell ...
Juan A. Lara, Alma Burciaga-Monge, Angel Chávez, Marc Revés, Rodolfo Lavilla, Monserrat Arró, Monserrat Arró, Albert Boronat, Albert Boronat, Teresa Altabella, Teresa Altabella, Albert Ferrer, Albert Ferrer   +12 more
doaj   +1 more source

AGPAT2 interaction with CDP-diacylglycerol synthases promotes the flux of fatty acids through the CDP-diacylglycerol pathway

Nature Communications, 2021
AGPATs (1-acylglycerol-3-phosphate O-acyltransferases) catalyze the acylation of lysophosphatidic acid to form phosphatidic acid (PA), a key step in the synthesis of all glycerolipids.
Hoi Yin Mak, Qian Ouyang, Sergey Tumanov, Jiesi Xu, Ping Rong, Feitong Dong, Sin Man Lam, Xiaowei Wang, Ivan Lukmantara, Ximing Du, Mingming Gao, Andrew J. Brown, Xin Gong, Guanghou Shui, Roland Stocker, Xun Huang, Shuai Chen, Hongyuan Yang   +17 more
doaj   +1 more source

Structural Basis for the Acyltransferase Activity of Lecithin:Retinol Acyltransferase-like Proteins [PDF]

Journal of Biological Chemistry, 2012
Lecithin:retinol acyltransferase-like proteins, also referred to as HRAS-like tumor suppressors, comprise a vertebrate subfamily of papain-like or NlpC/P60 thiol proteases that function as phospholipid-metabolizing enzymes. HRAS-like tumor suppressor 3, a representative member of this group, plays a key role in regulating triglyceride accumulation and ...
Marcin, Golczak, Philip D, Kiser, Avery E, Sears, David T, Lodowski, William S, Blaner, Krzysztof, Palczewski   +5 more
openaire   +2 more sources