Results 61 to 70 of about 6,281,815 (256)

Measurement of adaptive laboratory evolution experiment and dry cell weight

, 2023
This supplementary data is used as an appendix for BENG0046 report entitled "Enhancing the capabilities of non-conventional microbial hosts for industrial biomanufacturing". Appendix D.
Mario Torres Acosta (6780509), Duygu Dikicioglu (8690973), Waritthorn Thanakarn (13858136)   +2 more
core   +1 more source

Adaptation of Escherichia coli to Long-Term Serial Passage in Complex Medium: Evidence of Parallel Evolution

mSystems, 2017
Experimental evolution of bacterial populations in the laboratory has led to identification of several themes, including parallel evolution of populations adapting to carbon starvation, heat stress, and pH stress. However, most of these experiments study
Karin E. Kram, Christopher Geiger, Wazim Mohammed Ismail, Heewook Lee, Haixu Tang, Patricia L. Foster, Steven E. Finkel   +6 more
doaj   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

FEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens, Brendan W. Chandler, Anna M. Schmoker, Jaye L. Weinert, Charlotte A. Kearns, Warren T. Yacawych, Amila Šemić, Alicia M. Ebert, Bryan A. Ballif   +8 more
wiley   +1 more source

Adaptive laboratory evolution of a genome-reduced Escherichia coli

Nature Communications, 2019
Genome-reduced bacteria often show impaired growth under laboratory conditions. Here the authors use adaptive laboratory evolution to optimise growth performance and show transcriptome and translatome-wide remodeling of the organism.
Donghui Choe, Jun Hyoung Lee, Minseob Yoo, Soonkyu Hwang, Bong Hyun Sung, Suhyung Cho, Bernhard Palsson, Sun Chang Kim, Byung-Kwan Cho   +8 more
doaj   +1 more source

Adaptive laboratory evolution triggers pathogen-dependent broad-spectrum antimicrobial potency in Streptomyces

Journal of Genetic Engineering and Biotechnology, 2022
Background In the present study, adaptive laboratory evolution was used to stimulate antibiotic production in a Streptomyces strain JB140 (wild-type) exhibiting very little antimicrobial activity against bacterial pathogens.
Dharmesh Harwani, Jyotsna Begani, Sweta Barupal, Jyoti Lakhani   +3 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Comparative analysis of energy homeostasis regulation at different altitudes in Hengduan Mountain of red-backed vole, Eothenomys miletus, during high-fat diet acclimation: examining gut microbial and physiological interactions

Frontiers in Microbiology
The study aimed to explore the similarities and differences in gut microorganisms and their functions in regulating body mass in Eothenomys miletus across different altitudes in the Hengduan Mountains when exposed to a high-fat diet.
Ting Jia, Wei Zhang, Lijuan Cao, Lijuan Cao, Lijuan Cao, Wanlong Zhu, Wanlong Zhu, Wanlong Zhu, Lixian Fan   +8 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Physiological adaptation strategies for thermoregulation in Tupaia belangeri under high-temperature environment challenge

Frontiers in Physiology
IntroductionTo investigate the capacity of Tupaia belangeri to withstand high-temperature environments and its adaptability to global warming trends, while examining evidence for the species’ tropical origins through thermal neutral zone analysis ...
Dongjie Liu, Wanlong Zhu, Wanlong Zhu, Wanlong Zhu   +3 more
doaj   +1 more source