Results 121 to 130 of about 19,445 (257)

A Modular Nucleoside Kinase Cascade for the Synthesis of Ribonucleoside Triphosphates

Biotechnology and Bioengineering, EarlyView.
Damm et al. report standardized enzymatic cascades for production of all canonical nucleoside triphosphates from the corresponding nucleosides, using kinase‐catalyzed phosphorylation from acetyl phosphate. Reactions afforded ∼ 100 mM product in near‐quantitative conversion.
Oliver T. Damm, Martin Pfeiffer, Johannes Zöhrer, Bernd Nidetzky   +3 more
wiley   +1 more source

Translating Blue Light Stimulation From Batch to Perfusion: Process and Intracellular Metabolic Analysis

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Improving cell‐specific productivity remains a central objective in biopharmaceutical manufacturing. In this study, the effect of blue light illumination on IgG1‐producing CHO DP‐12 cells was systematically evaluated across batch, fed‐batch, and perfusion cultivations in controlled 3 L bioreactor systems.
Stefanie Föller, Attila Teleki, Ralf Takors   +2 more
wiley   +1 more source

Correction of human nonsense mutation via adenine base editing for Duchenne muscular dystrophy treatment in mouse

Molecular Therapy: Nucleic Acids
Duchenne muscular dystrophy (DMD) is the most prevalent herediatry disease in men, characterized by dystrophin deficiency, progressive muscle wasting, cardiac insufficiency, and premature mortality, with no effective therapeutic options.
Ming Jin, Jiajia Lin, Haisen Li, Zhifang Li, Dong Yang, Yin Wang, Yuyang Yu, Zhurui Shao, Long Chen, Zhiqiang Wang, Yu Zhang, Xiumei Zhang, Ning Wang, Chunlong Xu, Hui Yang, Wan-Jin Chen, Guoling Li   +16 more
doaj   +1 more source

The pAblo·pCasso self-curing vector toolset for unconstrained cytidine and adenine base-editing in Gram-negative bacteria

A synthetic biology toolkit, exploiting clustered regularly interspaced short palindromic repeats (CRISPR) and modified CRISPR-associated protein (Cas) base-editors, was developed for genome engineering in Gram-negative bacteria.
Nieto-Domínguez, Manuel, Nikel, Pablo I., Nielsen, Zacharias S., Kozaeva, Ekaterina   +3 more
core   +1 more source

Reprogramming tumor immune microenvironment by ultrasound‐responsive nanoplatforms for enhanced cancer immunotherapy

BMEMat, EarlyView.
Ultrasound‐responsive nanoplatforms reprogram the tumor immune microenvironment by targeting tumor cells, immune cells, and non‐immune stromal cells to enhance the efficacy of cancer immunotherapy. Abstract Cancer immunotherapy represents a significant advancement in cancer treatment by enhancing the specific recognition and elimination of cancer cells.
Shilong Zhao, Rui Qu, Zan Dai, Xiqun Jiang, Xu Zhen   +4 more
wiley   +1 more source

A nanoplatform that induces dual‐amino acid deprivation to reverse tumor immunosuppression and enhance metabolic immunotherapy

BMEMat, EarlyView.
ZIF‐8‐based nanoparticles co‐delivering CB‐839 (glutaminase inhibitor) and 1‐MT (IDO1 inhibitor), dual‐targeting glutamine/tryptophan metabolism to induce immunogenic cell death, activate STING, block kynurenine production, reverse immunosuppression, and enhance cancer immunotherapy to suppress primary/distant tumors.
Wenli Ning, Yunhan Sun, Mingyang Li, Shilu Liu, Fangjie Yi, Hongfei Wang, Ruibing Bai, Dongdong Liu, Fen Zhao, Guiqiang Zhang, Xiao Fu   +10 more
wiley   +1 more source

LNP‐mediated in vivo base editing corrects Agxt to cure primary hyperoxaluria type 1

Clinical and Translational Medicine
Objective Primary hyperoxaluria type 1 (PH1) is a rare autosomal recessive disorder caused by AGXT mutations, leading to hepatic oxalate overproduction, nephrolithiasis, and progressive renal failure. This study aims to evaluate the therapeutic potential
Dexin Zhang, Rui Zheng, Zhoutong Chen, Xi Chen, Lei Yang, Yanan Huo, Yining Zhao, Jiaxin Huang, Dan Zhang, Shuming Yin, Dali Li, Hongquan Geng   +11 more
doaj   +1 more source

Base Editor Correction of COL7A1 in Recessive Dystrophic Epidermolysis Bullosa Patient-Derived Fibroblasts and iPSCs

, 2020
Genome editing represents a promising strategy for the therapeutic correction of COL7A1 mutations that cause recessive dystrophic epidermolysis bullosa (RDEB).
Webber, Beau R., Osborn, Mark J., Eide, Cindy R., Nielsen, Sarah C., Liu, David R., Dabelsteen, Sally, Blazar, Bruce R., Knipping, Friederike, Chen, Weili, Riddle, Megan J., Xia, Lily, Newby, Gregory A., Wandall, Hans H., Tolar, Jakub, McElroy, Amber N.   +14 more
core   +1 more source

RNA‐centric world of retroviruses: unravelling the molecular strategies of genomic RNA packaging

Biological Reviews, EarlyView.
ABSTRACT Retroviruses constitute a unique group of RNA viruses that have profoundly influenced both evolutionary trajectories and biomedical research. Their ability to reverse transcribe and integrate into host genomes has shaped genomic architecture across species and contributed to our understanding of oncogenes, gene regulation, and RNA biology ...
Mohammad Abdullah Jehad, Suresha G. Prabhu, Lizna M. Ali, Vineeta N. Pillai, Farah Mustafa, Tahir A. Rizvi   +5 more
wiley   +1 more source

Structural and functional insights into internal domain replacement in SpCas9 for protein engineering

Scientific Reports
The CRISPR-Cas9 system has emerged as a powerful tool for precise genome editing, with ongoing research focused on enhancing its reliability and expanding its versatility. One effective strategy involves the integration of foreign functional domains into
Seonhong Kim, Hyuk Won, Jungnam Bae, Jeesoo Kim, Jinhyuk Choi, Halimriadi Richar, Yang-Gyun Kim, Hee-Jung Choi   +7 more
doaj   +1 more source