Results 151 to 160 of about 19,445 (257)

An innovative approach using CRISPR-ribonucleoprotein packaged in virus-like particles to generate genetically engineered mouse models

Nature Communications
Genetically engineered mouse models (GEMMs) are crucial for investigating disease mechanisms, developing therapeutic strategies, and advancing fundamental biological research. While CRISPR gene editing has greatly facilitated the creation of these models,
Tae Yeong Jeong, Da Eun Yoon, Sol Pin Kim, Jiyun Yang, Soo-Yeon Lim, Sungjin Ok, Sungjin Ju, Jeongeun Park, Su Bin Lee, Soo-Ji Park, Sanghun Kim, Hyunji Lee, Daekee Lee, Soo Kyung Kang, Seung Eun Lee, Hyeon Soo Kim, Je Kyung Seong, Kyoungmi Kim   +17 more
doaj   +1 more source

Development of a high-fidelity Cas9-dependent adenine base editor (ABE) system for genome editing with high-fidelity Cas9 variants. [PDF]

Genes Dis, 2023
Hong R, He X.
europepmc   +1 more source

The DIKW of Transcriptomics in Ecotoxicology: Extracting Information, Knowledge, and Wisdom From Big Data

Environmental Toxicology and Chemistry, EarlyView.
Jessica A. Head, Jessica D. Ewald, Niladri Basu   +2 more
wiley   +1 more source

METTL14 Ameliorates Mitochondrial Dysfunction and Autophagy in Lens Epithelial Cells of Diabetic Cataracts via m6A Modification of RPL3

The Kaohsiung Journal of Medical Sciences, EarlyView.
ABSTRACT Diabetic cataracts are a leading cause of blindness, with lens epithelial cells (LECs) exhibiting mitochondrial dysfunction and autophagy inhibition under high glucose (HG) conditions. Methyltransferase‐like 14 (METTL14), an RNA methyltransferase, regulates N6‐methyladenosine (m6A) RNA modification; however, its role in modulating ...
Rui Li, Xue Wang, Wei Du, Qian Li, Hong‐Ping Cui   +4 more
wiley   +1 more source

Mass Spectrometry Insights Into Post‐Translational Modifications in Extracellular Vesicles

Mass Spectrometry Reviews, EarlyView.
ABSTRACT Extracellular vesicles (EVs) are membrane‐enclosed structures secreted by virtually all living cells, serving as essential mediators of intercellular communication in both physiological and pathological processes. There is growing interest in their potential applications as biomarkers, therapeutic targets, and drug delivery systems, which ...
Dávid Virág, Krisztián Márk Karvaly, Alexandra Molnár, Mirjam Balbisi, Júlia Németh, Lilla Turiák   +5 more
wiley   +1 more source

Rationalizing the Effect of Mutations on the Editing Efficiency of Adenine Base Editors [PDF]

Biophysical Journal, 2020
Kartik Lakshmi Rallapalli, Francesco Paesani, Alexis Komor   +2 more
openaire   +1 more source

Prime editing in neuropsychiatric disorders: From mutation‐specific target selection to clinical translation

Neuroprotection, EarlyView.
Abstract Prime editing, a novel clustered regularly interspaced short palindromic repeats (CRISPR)‐based technology, fuses a reverse transcriptase (RT) to an engineered CRISPR‐associated protein 9 (Cas9) and uses a prime editing guide RNA (pegRNA)‐encoded template.
Tianshan Ji, Yuan Zhang, Jinyi Zhao, Yiping Lu, Chengkun Wang   +4 more
wiley   +1 more source

In vivo adenine base editing of mutant Galc gene ameliorates Krabbe disease progression

Genome Medicine
Background Krabbe disease (KD) is caused by mutation of the galactosylceramidase (GALC) gene, leading to deficient sphingolipid metabolism, which is essential for functional myelination.
Bae-Geun Nam, Jung Hwa Seo, Sung-Ah Hong, Ju-Hee Kim, Minju Kang, Geneva Rose Notario, Yoontaik Hong, Seunghee Cho, Sangsu Bae, Sung-Rae Cho   +9 more
doaj   +1 more source

Adenine base editing rescues pathogenic phenotypes in tissue engineered vascular model of Hutchinson-Gilford progeria syndrome

The rare, accelerated aging disease Hutchinson-Gilford Progeria Syndrome (HGPS) is commonly caused by a de novo c.1824 C > T point mutation of the LMNA gene that results in the protein progerin.
Akhil Bedapudi, George A. Truskey, Nadia O. Abutaleb, Crystal Kennedy, David R. Liu, Xin D. Gao, Jordyn E. Duby, Kevin L. Shores, Kan Cao, Leandro Choi   +9 more
core   +1 more source

The Mycobacterium tuberculosis Rv0132c Gene Product Mtb‐FGD2 Can Act as an F420‐Dependent Glucose Dehydrogenase

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT The role of the cell envelope‐associated Rv0132c/FGD2 from Mycobacterium tuberculosis has long been a subject of debate. Importantly, FGD2 is found only in pathogenic mycobacteria, making it a potential drug target. While some suggest it functions as a glucose‐6‐phosphate dehydrogenase, others propose it acts instead as an F420‐dependent ...
Adewale V. Aderemi, Matthew Snee, Richard B. Tunnicliffe, Linus O. Johanissen, Matthew J. Cliff, Colin W. Levy, Derren J. Heyes, Marina Golovanova, Thomas A. Jowitt, Sam Hay, Andrew W. Munro, Jonathan P. Waltho, David Leys   +12 more
wiley   +1 more source