On the Calculations of Electron Impact Ionization Cross-Sections for Selected Nucleosides and Deoxyribose Molecules. [PDF]
MoleculesMożejko P.
europepmc +1 more source
<i>In vivo</i> adenine base editing rescues brain biochemistry and improves motor deficits in a common phenylketonuria variant. [PDF]
Mol Ther AdvGautier MK +8 more
europepmc +1 more source
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Heterologous Expression and Purification of a CRISPR-Cas9-Based Adenine Base Editor
Methods in Molecular Biology, 2023CRISPR-cas9-guided adenine base editors (ABEs) site-specifically convert the A-T base pair to G-C base pair in genomic DNA. The intracellular delivery of ABE proteins preassembled with guide RNAs (gRNAs) has shown greatly reduced off-target effects compared with that of plasmids or viral vectors containing ABE and gRNA-encoding sequences. For efficient
Seu-Na Lee, Jae-Sung Woo
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Dual base editor catalyzes both cytosine and adenine base conversions in human cells
Nature Biotechnology, 2020Although base editors are useful tools for precise genome editing, current base editors can only convert either adenines or cytosines. We developed a dual adenine and cytosine base editor (A&C-BEmax) by fusing both deaminases with a Cas9 nickase to achieve C-to-T and A-to-G conversions at the same target site. Compared to single base editors, A&C-BEmax'
Mingyao Liu, Yukio Nakamura, Li Yang
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Adenine base editors catalyze cytosine conversions in human cells
Nature Biotechnology, 2019Adenine base editors comprise an adenosine deaminase, evolved in vitro, and a Cas9 nickase. Here, we show that in addition to converting adenine to guanine, adenine base editors also convert cytosine to guanine or thymine in a narrow editing window (positions 5-7) and in a confined TC*N sequence context.
Heon Seok Kim +4 more
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An adenine base editor variant expands context compatibility
Nature BiotechnologyAdenine base editors (ABEs) are precise gene-editing agents that convert A:T pairs into G:C through a deoxyinosine intermediate. Existing ABEs function most effectively when the target A is in a TA context. Here we evolve the Escherichia coli transfer RNA-specific adenosine deaminase (TadA) to generate TadA8r, which extends potent deoxyadenosine ...
Yu-Lan Xiao, Yuan Wu, Weixin Tang
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Genome Engineering Human ESCs or iPSCs with Cytosine and Adenine Base Editors
2022The ability to engineer specific mutations in human embryonic stem cells (ECSs) or induced pluripotent stem cells (iPSCs) is extremely important in the modeling of human diseases and the study of biological processes. While CRISPR/Cas9 can robustly generate gene knockouts (KOs) and gene loci modifications in coding sequences of iPSCs, it remains ...
Giulia, Pavani +3 more
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Germline Editing of Drosophila Using CRISPR-Cas9-Based Cytosine and Adenine Base Editors
The CRISPR Journal, 2023Target-AID, BE3, and ABE7.10 base editors fused to the catalytically modified Cas9 and xCas9(3.7) were tested for germline editing of the fruit fly Drosophila melanogaster . We developed a guide RNA-expressing construct, white- 4gRNA, targeting splice ...
Nirav Thakkar +3 more
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