Results 41 to 50 of about 865,113 (239)

Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction

Nature Biotechnology, 2018
Base editors enable targeted single-nucleotide conversions in genomic DNA. Here we show that expression levels are a bottleneck in base-editing efficiency.
Luke W. Koblan, Jordan L. Doman, Christopher Wilson, Jonathan M. Levy, Tristan Tay, Gregory A. Newby, J. P. Maianti, Aditya Raguram, David R. Liu   +8 more
semanticscholar   +1 more source

Substrate Nucleotide-Determined Non-Templated Addition of Adenine by Taq DNA Polymerase: Implications for PCR-Based Genotyping and Cloning

BioTechniques, 1996
The Applied Biosystems PRISMTM fluorescence-based genotyping system as well as the Invitrogen TA Cloning® vector system are influenced by the tendency of Taq DNA polymerase to add an adenine nucleotide to the 3′ end of PCR products after extension ...
V.L. Magnuson, D.S. Ally, S.J. Nylund, Z.E. Karanjawala, J.B. Rayman, J.I. Knapp, A.L. Lowe, S. Ghosh, F.S. Collins   +8 more
doaj   +1 more source

Probabilistic models of biological enzymatic polymerization.

PLoS ONE, 2021
In this study, hierarchies of probabilistic models are evaluated for their ability to characterize the untemplated addition of adenine and uracil to the 3' ends of mitochondrial mRNAs of the human pathogen Trypanosoma brucei, and for their generative ...
Marshall Hampton, Miranda Galey, Clara Smoniewski, Sara L Zimmer   +3 more
doaj   +1 more source

Protocol for targeted modification of the rice genome using base editing

STAR Protocols, 2022
Summary: Base editing is a precision genome-editing approach that is widely utilized to generate single-nucleotide variants (SNVs) in genomes. Here, we present a protocol to perform targeted adenine (A)-to-guanine (G) substitution in rice using adenine ...
Meixia Wang, Fang Yan, Huanbin Zhou
doaj   +1 more source

Interaction of low-energy electrons with the purine bases, nucleosides, and nucleotides of DNA [PDF]

, 2006
The authors report results from computational studies of the interaction of low-energy electrons with the purine bases of DNA, adenine and guanine, as well as with the associated nucleosides, deoxyadenosine and deoxyguanosine, and the nucleotide ...
McKoy, Vincent, Winstead, Carl
core   +1 more source

Adenine Nucleotide Translocase, Mitochondrial Stress, and Degenerative Cell Death

Oxidative Medicine and Cellular Longevity, 2013
Mitochondria are intracellular organelles involved in ATP synthesis, apoptosis, calcium signaling, metabolism, and the synthesis of critical metabolic cofactors. Mitochondrial dysfunction is associated with age-related degenerative diseases.
Yaxin Liu, Xin Jie Chen
semanticscholar   +1 more source

Single nucleotide polymorphisms from Theobroma cacao expressed sequence tags associated with witches' broom disease in cacao [PDF]

, 2009
In order to increase the efficiency of cacao tree resistance to witches¿ broom disease, which is caused by Moniliophthora perniciosa (Tricholomataceae), we looked for molecular markers that could help in the selection of resistant cacao genotypes.
Carels, Nicolas, Cascardo, Julio Cezar M., Gaiotto, Fernanda Amato, Gesteira, Abelmon, Gramacho, Karina Peres, Lima, Leila S., Lopes, Uilson Vanderlei, Micheli, Fabienne, Novais, R., Pires, José Luis, Zaidan, Humberto A.   +10 more
core   +1 more source

The ATP/ADP translocase drives mitophagy independent of nucleotide exchange

Nature, 2019
Mitochondrial homeostasis depends on mitophagy, the programmed degradation of mitochondria. Only a few proteins are known to participate in mitophagy.
Atsushi Hoshino, Wei-jia Wang, Shogo Wada, Chris McDermott-Roe, Chantell S. Evans, Bridget S. Gosis, M. Morley, Komal S. Rathi, Jian Li, Kristina Li, S.T. Yang, Meagen J. McMannus, Caitlyn E. Bowman, P. Potluri, Michael G. Levin, Scott Damrauer, D. Wallace, E. Holzbaur, Z. Arany   +18 more
semanticscholar   +1 more source

Genetic Predisposition To Acquire a Polybasic Cleavage Site for Highly Pathogenic Avian Influenza Virus Hemagglutinin

mBio, 2017
Highly pathogenic avian influenza viruses with H5 and H7 hemagglutinin (HA) subtypes evolve from low-pathogenic precursors through the acquisition of multiple basic amino acid residues at the HA cleavage site. Although this mechanism has been observed to
Naganori Nao, Junya Yamagishi, Hiroko Miyamoto, Manabu Igarashi, Rashid Manzoor, Aiko Ohnuma, Yoshimi Tsuda, Wakako Furuyama, Asako Shigeno, Masahiro Kajihara, Noriko Kishida, Reiko Yoshida, Ayato Takada   +12 more
doaj   +1 more source

The Human SLC25A33 and SLC25A36 Genes of Solute Carrier Family 25 Encode Two Mitochondrial Pyrimidine Nucleotide Transporters [PDF]

, 2014
The human genome encodes 53 members of the solute carrier family 25 (SLC25), also called the mitochondrial carrier family, many of which have been shown to transport inorganic anions, amino acids, carboxylates, nucleotides, and coenzymes across the inner
Agrimi, G., Cirigliano, A., Di Noia, M. A., Iacobazzi, V., Palmieri, Ferdinando, Rinaldi, T., Todisco, S.   +6 more
core   +2 more sources