Results 131 to 140 of about 6,350 (165)
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Studies of the binding of adenine to adenine phosphoribosyltransferase
Canadian Journal of Biochemistry, 1970The groups on adenine phosphoribosyltransferase and on adenine which are involved in the binding of this substrate were investigated by comparing the inhibition constant of adenine with those obtained for analogues of adenine, by determining the effect of pH on the Michaelis constant of adenine, and by protection by adenine against enzyme inactivation
Henderson Jf, Gadd Re
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A spectrophotometric assay for adenine phosphoribosyltransferase
Analytical Biochemistry, 1977Abstract The present paper describes a new spectrophotometric assay for adenine phosphoribosyltransferase activity which is highly reproducible, rapid, sensitive, and simpler than the isotopic assays for this enzyme. This assay is based on the quantitative measurement of the product AMP by a NADH-coupled enzyme method.
SALERNO, Costantino +1 more
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Hysteretic characteristic of adenine phosphoribosyltransferase
Biochemistry, 1975Preassay-incubation of the highly purified human erythrocyte adenine phosphoribosyltransferase (EC 2.4.2.7) (AMP pyrophosphorylase) with one of its substrates, 5-phosphoribosyl 1-pyrophosphate (PRibPP), changes the apparent V max value of the enzyme reaction.
Balis Me, Yip Lc
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Adenine Phosphoribosyltransferase Deficiency in Iceland
Acta Medica Scandinavica, 1988Abstract. Two children and two adults of four unrelated families were on regular light microscopic examination found to exhibit identical, spherical urine crystals. Their characteristic appearance led to the diagnosis of 2,8‐dihydroxyadenine crystalluria by spectrophotometric or gas‐chromatographic/mass‐spectrometric analysis.
Tómas Á. Jónasson, Thröstur Laxdal
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Inheritance of Adenine Phosphoribosyltransferase (APRT) Deficiency [PDF]
, 1980 Recognition of the importance of the enzyme hypoxanthine-guanine phosphoribosyltransferase (HGPRT) in the control of purine metabolism lead to systematic investigations of the companion purine salvage enzyme, adenine phosphoribosyltransferase (APRT). This was followed by the discovery of individuals (considered to be heterozygous for the defect) with ...
K. J. Van Acker +7 more
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Adenine phosphoribosyltransferase deficiency in children
Pediatric Nephrology, 2012Adenine phosphoribosyltransferase (APRT) deficiency is a rare autosomal recessive disorder characterized by 2,8-dihydroxyadenine (2,8-DHA) crystalluria that can cause nephrolithiasis and chronic kidney disease. The aim of our study was to assess the clinical presentation, diagnosis, and outcome of APRT deficiency in a large pediatric cohort.
Jérôme Harambat +4 more
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Characterization of an adenine phosphoribosyltransferase deficiency
Clinica Chimica Acta, 1988A case of adenine phosphoribosyltransferase deficiency in a 4.5-yr-old boy is described. A pedigree of the family, enzyme activities and kinetic data of the enzyme in the propositus and the carriers of the defect are presented. The amount of enzyme in the patient was about 2% of that in healthy subjects and correlated well with the amount of ...
K. Zwiauer, P. Chiba, Mathias Müller
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Mechanisms of inhibition of adenine phosphoribosyltransferase by adenine nucleosides and nucleotides
Canadian Journal of Biochemistry, 1970Kinetic studies of the inhibition of adenine phosphoribosyltransferase by adenine 6′-deoxyallofuranoside and 2′-deoxyadenylate indicate that both compounds bind to free enzyme and to the enzyme–phosphoribosylpyrophosphate complex, although they bind with different relative affinities to each enzyme form. The sites to which these inhibitors bind appear
Henderson Jf, Hori M, Palser Hm, Gadd Re
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On the formation of an intermediate in the adenine phosphoribosyltransferase reaction
Biochemical and Biophysical Research Communications, 1971Summary Highly purified rat liver adenine phosphoribosyltransferase (EC 2.4. 2.7) exhibits an “initial burst” reaction in the presence of either PP-ribose-P or Mg-ADP indicating that the purified enzyme contains a bound phosphoribosyl group. The initial burst reaction was studied separately by inhibiting the overall reaction between PP-ribose-P and ...
D.P. Groth, L.G. Young
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[77] Adenine phosphoribosyltransferase
1978Publisher Summary This chapter describes the assay method of adenine phosphoribosyltransferase (APRT) enzyme. Human APRT catalyzes the magnesium-dependent transfer of the ribose-5-phosphate moiety of 5-phosphoribosyl-l-pyrophosphate (PP-ribose-P) to the 9 position of the purine base adenine to form adenosine-5'-monophosphate (AMP).
William J. Arnold, William N. Kelley
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