Results 41 to 50 of about 70,698 (266)

Mutant p53R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation

Molecular Oncology, EarlyView.
How mutant p53R273H drives AKT signaling is unclear. We show that p53R273H, but not wild‐type, directly binds PDPK1 via a mutation‐dependent conformational change. This interaction disrupts inhibitory PDPK1 homodimerization and enhances AKT phosphorylation.
Mei Chee Lim, Alvin Kunyao Guo, Yoko Itahana, Srinivasaraghavan Kannan, Valerie Goh, Mingmin Ong, Terina Yan Wen Tay, Weiying Ye, Sabhashina Radha Krishnan, Jun Nishiyama, Chandra S Verma, Koji Itahana   +11 more
wiley   +1 more source

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

FEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks, Garth L. Maker, Emily C. Davies, Berin A. Boughton, Samantha Lodge   +4 more
wiley   +1 more source

Microfluidic Droplet Plate Preserving Single Human Induced Pluripotent Stem Cell‐Derived Cardiomyocytes Morphology and Function

Advanced Materials Technologies, EarlyView.
We introduce a novel method combining microfluidics, optogenetics, and human induced pluripotent stem cell (iPSC) technology to encapsulate single iPSC‐derived cardiomyocytes (iPSC‐CMs) in an optimal environment for functional characterization. This method enables direct genotype‐phenotype correlation for genetic studies and high‐throughput screening ...
Xiao‐Ting Wang, Rachel P. White, Yashasvi Tharani, Michael A. Finocchiaro, Leona M. Ripple, Maksymilian Prondzynski, Tania Konry, Vassilios J. Bezzerides   +7 more
wiley   +1 more source

A Rare De Novo Missense Mutation in IFT122 Confers a Genetic Susceptibility Factor of Idiopathic Pediatric Uveitis Via Trio‐based Whole‐Exome Sequencing

Advanced Science, EarlyView.
A rare de novo IFT122‐A773E variant is identified in idiopathic pediatric uveitis and shown to exacerbate retinal inflammation and barrier dysfunction. Mechanistically, the variant enhances IFT43 interaction, elevates calcium signaling, and activates the MEK/ERK/FRA1 axis, revealing a previously unrecognized cilia‐associated pathway that may increase ...
Qian Zhou, Jiaxing Huang, Yilin Wang, Xingran Li, Xianyang Liu, Lingyu Dai, Hongshun Li, Qingfeng Wang, Jiangyi Liu, Guannan Su, Wanyun Zhang, Yang Deng, Qingfeng Cao, Yujie Lai, Xiang Luo, Changwei Huang, Ling Chen, Shengping Hou, Peizeng Yang   +18 more
wiley   +1 more source

Adenoviral vector‐based platforms for developing effective vaccines to combat respiratory viral infections

Clinical & Translational Immunology, 2021
Since the development of the first vaccine against smallpox over two centuries ago, vaccination strategies have been at the forefront of significantly impacting the incidences of infectious diseases globally. However, the increase in the human population,
Ahmed Elkashif, Marwa Alhashimi, Ekramy E Sayedahmed, Suryaprakash Sambhara, Suresh K Mittal   +4 more
doaj   +1 more source

Determinants of Outcomes of Adenoviral Keratoconjunctivitis [PDF]

Ophthalmology, 2018
To determine host and pathogen factors predictive of outcomes in a large clinical cohort with keratoconjunctivitis.Retrospective analyses of the clinical and molecular data from a randomized, controlled, masked trial for auricloscene for keratoconjunctivitis (NVC-422 phase IIB, NovaBay; clinicaltrials.gov identifier, NCT01877694).Five hundred ...
Cecilia S. Lee, Aaron Y. Lee, Lakshmi Akileswaran, David Stroman, Kathryn Najafi-Tagol, Steve Kleiboeker, James Chodosh, Amalia Magaret, Anna Wald, Russell N. Van Gelder, Gregg J. Berdy, James D. Branch, El-Roy Dixon, Sherif M. El-Harazi, Jack V. Greiner, Joshua Herz, Larry L. Lothringer, Damien Macaluso, Andrew L. Moyes, George Nardin, Bernard R. Perez, Lawerence E. Roel, Syamala H.K. Reddy, Stephanie Becker, Neil Shmunes, Stephen Smith, Michael Tepedino, Jonathan Macy, Prashant Garg, Nivedita Patil, Yasmin Bhagat, Malavika Krishnaswamy, Nagappa Somshekhar, Manisha Acharya, Shree Kumar Reddy, Mary Abraham, Shobha Kini, Nita Shanbag, P.N. Biswas, Virendra Agarwal, Anshu Sahai, P.S. Girija Devi, Vupputuri Venkata Lakshmi, Narasimha Rao, Radhika Tandon, Priti Kapadia, Deepak Mehta, Anju Kochar, Adriana dos Santos Forseto, Rubens Belfort, Jacob Moyses Cohen, Ramon Coral Ghanem, Roberta De Ventura, Sergio Luis Gianotti Pimentel, Sergio Kwitko, Maria Cristina Nishiwaki Dantas, Anna Maria Hofling-Lima, Walton Nose, D. Wariyapola, M. Wijetunge, Charith Fonseka, Champa Banagala, K.A. Salvin, D.R. Kodikara   +63 more
openaire   +2 more sources

Single‐Cell Profiling Reveals a Protective WNT5A‐ATF3‐FOSB Signaling Axis in Hair Follicle Stem Cells During Androgenetic Alopecia

Advanced Science, EarlyView.
Androgenetic alopecia (AGA) is a common form of hair loss with limited treatment options. Silencing of WNT5A signaling, which is widely known as the trigger of the ncWNT signaling pathway, happens in hair follicle stem cells from balding areas. It leads to downregulation of ATF3 and its target FOSB.
Ruiyu Luo, Ke Fang, Changjiang Zhao, Jiachao Xiong, Yuan Zhu, Mingyang Lu, He Yan, Zihan Li, Baojin Wu, Hua Jiang, Yufei Li   +10 more
wiley   +1 more source

Adenovirus vectors can infect mouse megakaryocytes – implications for vaccine-induced thrombosis/thrombocytopenia

Research and Practice in Thrombosis and Haemostasis
Background: Adenoviruses, adenoviral vector vaccines, and gene delivery systems have been implicated in thrombosis/thrombocytopenia syndrome. The underlying cause remains unknown.
Simon Collett, Linda Earnest, Melissa A. Edeling, Ashley Huey Yiing Yap, Julio Carrera Montoya, Chantal Attard, Ladina Di Rago, Alison Farley, Ashley P. Ng, Paul Monagle, Joseph Torresi   +10 more
doaj   +1 more source

Isolation and Characterization of Anti-Adenoviral Secondary Metabolites from Marine Actinobacteria

Marine Drugs, 2014
Adenovirus infections in immunocompromised patients are associated with high mortality rates. Currently, there are no effective anti-adenoviral therapies available.
Mårten Strand, Marcus Carlsson, Hanna Uvell, Koushikul Islam, Karin Edlund, Inger Cullman, Björn Altermark, Ya-Fang Mei, Mikael Elofsson, Nils-Peder Willassen, Göran Wadell, Fredrik Almqvist   +11 more
doaj   +1 more source

Inhibitors of metalloprotease, γ-sectretase, protein kinase C and Rho kinase inhibit wild-type adenoviral replication.

PLoS ONE, 2020
Adenoviruses cause upper respiratory infections, conjunctivitis, keratitis, and gastrointestinal illness. These can be fatal in immunocompromised individuals.
Alice Liu, Cristhian J Ildefonso, Wesley S Bond, Mary Y Hurwitz, Richard L Hurwitz   +4 more
doaj   +1 more source