Results 31 to 40 of about 39,421 (210)

High-Throughput Screening for the Prevalence of Neutralizing Antibodies against Human Adenovirus Serotype 5

Vaccines
Adenoviral vectors based on the human adenovirus species C serotype 5 (HAdV-C5) are commonly used for vector-based gene therapies and vaccines. In the preclinical stages of development, their safety and efficacy are often validated in suitable animal ...
Jochen M. Wettengel, Hiroaki Naka, Gregory A. Dissen, Jeffrey Torgerson, Michelle Pounder, Simon F. Mueller, Elisabeth Mueller, Philipp Hagen, Micah Brandt, Ulrike Protzer, Benjamin J. Burwitz   +10 more
doaj   +1 more source

A High-Capacity Adenoviral Hybrid Vector System Utilizing the Hyperactive Sleeping Beauty Transposase SB100X for Enhanced Integration

Molecular Therapy: Nucleic Acids, 2016
For efficient delivery of required genetic elements we utilized high-capacity adenoviral vectors in the past allowing high transgene capacities of up to 36 kb.
Philip Boehme, Wenli Zhang, Manish Solanki, Eric Ehrke-Schulz, Anja Ehrhardt   +4 more
doaj   +1 more source

Microfluidic Droplet Plate Preserving Single Human Induced Pluripotent Stem Cell‐Derived Cardiomyocytes Morphology and Function

Advanced Materials Technologies, EarlyView.
We introduce a novel method combining microfluidics, optogenetics, and human induced pluripotent stem cell (iPSC) technology to encapsulate single iPSC‐derived cardiomyocytes (iPSC‐CMs) in an optimal environment for functional characterization. This method enables direct genotype‐phenotype correlation for genetic studies and high‐throughput screening ...
Xiao‐Ting Wang, Rachel P. White, Yashasvi Tharani, Michael A. Finocchiaro, Leona M. Ripple, Maksymilian Prondzynski, Tania Konry, Vassilios J. Bezzerides   +7 more
wiley   +1 more source

A Novel Adenoviral Hybrid-vector System Carrying a Plasmid Replicon for Safe and Efficient Cell and Gene Therapeutic Applications

Molecular Therapy: Nucleic Acids, 2013
In dividing cells, the two aims a gene therapeutic approach should accomplish are efficient nuclear delivery and retention of therapeutic DNA. For stable transgene expression, therapeutic DNA can either be maintained by somatic integration or episomal ...
Richard Voigtlander, Rudolf Haase, Martin Mück-Hausl, Wenli Zhang, Philip Boehme, Hans-Joachim Lipps, Eric Schulz, Armin Baiker, Anja Ehrhardt   +8 more
doaj   +1 more source

Complementary adenoviral vectors for oncolysis [PDF]

Cancer Gene Therapy, 1999
Replication-competent adenoviruses (Ads) were used for oncolytic virotherapy soon after they were discovered. Recently mutated and genetically engineered Ads have been shown to selectively lyse tumor cells. We have split the human Ad type 5 genome into two defective viruses that complement each other only in certain tumor cells.
R, Alemany, S, Lai, Y C, Lou, H Y, Jan, X, Fang, W W, Zhang   +5 more
openaire   +2 more sources

A Rare De Novo Missense Mutation in IFT122 Confers a Genetic Susceptibility Factor of Idiopathic Pediatric Uveitis Via Trio‐based Whole‐Exome Sequencing

Advanced Science, EarlyView.
A rare de novo IFT122‐A773E variant is identified in idiopathic pediatric uveitis and shown to exacerbate retinal inflammation and barrier dysfunction. Mechanistically, the variant enhances IFT43 interaction, elevates calcium signaling, and activates the MEK/ERK/FRA1 axis, revealing a previously unrecognized cilia‐associated pathway that may increase ...
Qian Zhou, Jiaxing Huang, Yilin Wang, Xingran Li, Xianyang Liu, Lingyu Dai, Hongshun Li, Qingfeng Wang, Jiangyi Liu, Guannan Su, Wanyun Zhang, Yang Deng, Qingfeng Cao, Yujie Lai, Xiang Luo, Changwei Huang, Ling Chen, Shengping Hou, Peizeng Yang   +18 more
wiley   +1 more source

Development of novel vaccine vectors: Chimpanzee adenoviral vectors

Human Vaccines & Immunotherapeutics, 2018
Adenoviral vector has been employed as one of the most efficient means against infectious diseases and cancer. It can be genetically modified and armed with foreign antigens to elicit specific antibody responses and T cell responses in hosts as well as ...
Jingao Guo, Moumita Mondal, Dongming Zhou   +2 more
doaj   +1 more source

Single‐Cell Profiling Reveals a Protective WNT5A‐ATF3‐FOSB Signaling Axis in Hair Follicle Stem Cells During Androgenetic Alopecia

Advanced Science, EarlyView.
Androgenetic alopecia (AGA) is a common form of hair loss with limited treatment options. Silencing of WNT5A signaling, which is widely known as the trigger of the ncWNT signaling pathway, happens in hair follicle stem cells from balding areas. It leads to downregulation of ATF3 and its target FOSB.
Ruiyu Luo, Ke Fang, Changjiang Zhao, Jiachao Xiong, Yuan Zhu, Mingyang Lu, He Yan, Zihan Li, Baojin Wu, Hua Jiang, Yufei Li   +10 more
wiley   +1 more source

Fluorescently Labeled Adenovirus with pIX-EGFP for Vector Detection

Molecular Imaging, 2004
Adenoviruses are extensively studied in terms of their use as gene therapy vectors and pathogenesis. These vectors have been targeted on both transcriptional and transductional levels to achieve cell-specific gene delivery.
Long P. Le, Maaike Everts, Igor P. Dmitriev, Julia G. Davydova, Masato Yamamoto, David T. Curiel   +5 more
doaj   +1 more source

An Engineered HR1‐Stem Helix‐HR2 Trimeric Platform for Developing Broad‐Spectrum Coronavirus Vaccines

Advanced Science, EarlyView.
Sun et al. report a self‐assembling trimeric subunit vaccine platform termed RHS, which integrates the JN.1 receptor‐binding domain (RBD) with the heptad repeat 1 (HR1), stem helix (SH), and heptad repeat 2 (HR2) regions. The SARS‐CoV‐2 RHS vaccine elicits variant‐reactive RBD‐specific antibodies and cross‐reactive SH‐specific antibodies against ...
Xikui Sun, Junhao Fan, Xiaolu Xie, Yuting Shi, Xiaoqing Liu, Tiefeng Xu, Sidi Yang, Keda Shi, Chengshu Xie, Shixiong Li, Siwen Chen, Ruoxi Cai, Ping He, Yuzhen Ye, Tao Chen, Xiancai Ma, Chunmei Li, Jingyou Yu, Deyin Guo   +18 more
wiley   +1 more source