Results 201 to 210 of about 3,127,180 (269)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Correction to: The Centers for Medicare and Medicaid Services and others misunderstand stool testing for colorectal cancer. [PDF]

J Natl Cancer Inst
europepmc   +1 more source

Genomic characterization of a MAGIC population containing introgressions from four tetraploid Gossypium species. [PDF]

Plant Genome
Pinnika G, Li P, McCarty JC, Jenkins JN, Fang DD.   +4 more
europepmc   +1 more source

DEB-TACE Combined with Immunotherapy and Targeted Therapy for Advanced Hepatocellular Carcinoma with Scapular Metastasis: A Case Report. [PDF]

J Hepatocell Carcinoma
Zhang W, Kang D, Xu Q, Piao L.
europepmc   +1 more source

Spiritual wellbeing in advanced cancer patients receiving palliative care in a tertiary cancer centre in northern Kerala, India: a qualitative study. [PDF]

BMJ Open
Biji MS, Kc R, Nair S, Shenoy PK, Spruyt O, Venkateswaran C, B S, Vijayakumar DK.   +7 more
europepmc   +1 more source

The cost-effectiveness of medical versus surgical androgen deprivation therapy for advanced prostate cancer in Tanzania: a Markov model-based analysis. [PDF]

Front Public Health
Nyongole O, Tungu M, Kagaigai A, Anaeli A, Tindamanyile A, Njau P, Mizinduko M, Urassa D, Sunguya B, Frumence G.   +9 more
europepmc   +1 more source