Results 51 to 60 of about 1,074,495 (313)
Statistical deconvolution of enthalpic energetic contributions to MHC-peptide binding affinity [PDF]
, 2006 Background: MHC Class I molecules present antigenic peptides to cytotoxic T cells, which forms an integral part of the adaptive immune response. Peptides are bound within a groove formed by the MHC heavy chain. Previous approaches to MHC Class I-peptide
Hattotuwagama, C.K. +18 more
core +1 more source
Affinity chromatography and capillary electrophoresis for analysis of the yeast ribosomal proteins [PDF]
BMB Reports, 2012 We present a top down separation platform for yeast ribosomalproteins using affinity chromatography and capillary electrophoresiswhich is designed to allow deposition of proteins ontoa substrate. FLAG tagged ribosomes were affinity purified, andrRNA acid
Miriam S. Goyder +4 more
doaj +1 more source
Sensors, 2022 Affinity describes the non-covalent but selective interaction between an affinity binder (e.g., proteins, antibiotics, or antibodies) and its counterpart (e.g., bacteria).
Julia Neumair +2 more
doaj +1 more source
FEBS Letters, EarlyView.Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes +4 more
wiley +1 more source
Affinity as a tool in life science
BioTechniques, 2008 The use of affinity-based tools has become invaluable as a platform for basic research and in the development of drugs and diagnostics. Applications include affinity chromatography and affinity tag fusions for efficient purification of proteins as well ...
Mathias Uhlén
doaj +1 more source
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Overview of Affinity Biosensors in Food Analysis [PDF]
Journal of AOAC INTERNATIONAL, 2006 Abstract The 4 major driving forces that are expected to lead to increased use of affinity biosensors that meet crucial industrial test specifications, e.g., fast, reliable, cost-effective, and use of low-skilled personnel, are (1) strict legislative framework, e.g., recent changes proposed to the European food safety and hygiene ...
openaire +2 more sources
Analysis of Antibody Clonotype by Affinity Immunoblotting [PDF]
, 2015 A sensitive and specific method to analyze specific antibody clonotype changes in a lupus patient who developed autoantibodies to the Ro 60 autoantigen under observation is described. Patient sera, collected over several years, were separated by flatbed isoelectric focusing (IEF) and analyzed by affinity immunoblotting utilizing Ro 60-coated ...
Biji T, Kurien, R Hal, Scofield
openaire +2 more sources
Equi-affine Invariant Geometry for Shape Analysis [PDF]
Journal of Mathematical Imaging and Vision, 2013 zbMATH Open Web Interface contents unavailable due to conflicting licenses.
Raviv, Dan +5 more
openaire +3 more sources