Removal of dsRNA byproducts using affinity chromatography [PDF]
Molecular Therapy: Nucleic AcidsDouble-stranded RNA (dsRNA) molecules are immunogenic byproducts of in vitro transcription of single-stranded RNA (ssRNA). Removal of dsRNA from ssRNA is difficult because the byproducts have similar sizes, sequences, and charges to the desired ssRNA ...
Nathaniel E. Clark +8 more
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Kinetic Analysis by Affinity Chromatography [PDF]
Frontiers in Chemistry, 2019 Important information on chemical processes in living systems can be obtained by the rates at which these biological interactions occur. This review will discuss several techniques based on traditional and high-performance affinity chromatography that ...
Sazia Iftekhar +2 more
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High-efficiency purification of divergent AAV serotypes using AAVX affinity chromatography [PDF]
Molecular Therapy: Methods & Clinical Development, 2023 The adeno-associated viral vector (AAV) provides a safe and efficient gene therapy platform with several approved products that have marked therapeutic impact for patients.
Michael Florea +8 more
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Extending the Affinity Range of Weak Affinity Chromatography for the Identification of Weak Ligands Targeting Membrane Proteins [PDF]
Molecules, 2023 The identification of weak-affinity ligands targeting membrane proteins is of great interest in Fragment-Based Drug Design (FBDD). Recently, miniaturized weak affinity chromatography (WAC) has been proposed as a valuable tool to study interactions ...
Adrien Deloche +5 more
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Affinity chromatography of native and recombinant proteins-from receptors for insulin and IGF-I to recombinant single chain antibodies [PDF]
Frontiers in Endocrinology, 2015 Affinity chromatography is an efficient method to isolate proteins by taking advantage of their affinities for specific molecules such as substrates, inhibitors, antigens, ligands, antibodies, and other interacting molecules including subunits. Nowadays,
Yoko eFujita-Yamaguchi
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Ligand Screening of Membrane Proteins Embedded in Nanodiscs: How to Manage Non-Specific Interactions in Weak Affinity Chromatography? [PDF]
MoleculesMiniaturized weak affinity chromatography is emerging as an interesting alternative to conventional biophysical tools for performing fragment-screening studies in the context of fragment-based drug discovery.
François-Xavier Vidal +6 more
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Glycoform-resolved FcɣRIIIa affinity chromatography–mass spectrometry [PDF]
mAbs, 2019 Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.
Steffen Lippold +8 more
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Non-targeted characterization of attributes affecting antibody-FcγRIIIa V158 (CD16a) binding via online affinity chromatography-mass spectrometry [PDF]
mAbs, 2022 Antibodies facilitate targeted cell killing by engaging with immune cells such as natural killer cells through weak binding interactions with Fcγ receptors on the cell surface.
Daniel W. Woodall +6 more
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Snorkel‐tag based affinity chromatography for recombinant extracellular vesicle purification [PDF]
Journal of Extracellular VesiclesExtracellular vesicles (EVs) are lipid nanoparticles and play an important role in cell‐cell communications, making them potential therapeutic agents and allowing to engineer for targeted drug delivery.
Madhusudhan Reddy Bobbili +16 more
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High-grade extracellular vesicles preparation by combined size-exclusion and affinity chromatography [PDF]
Scientific Reports, 2021 Extracellular vesicles (EVs) have recently gained growing interest for their diagnostic and therapeutic potential. Despite this, few protocols have been reported for the isolation of EVs with preserved biological function.
Cristina Bellotti +4 more
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