Removal of dsRNA byproducts using affinity chromatography [PDF]
Molecular Therapy: Nucleic AcidsDouble-stranded RNA (dsRNA) molecules are immunogenic byproducts of in vitro transcription of single-stranded RNA (ssRNA). Removal of dsRNA from ssRNA is difficult because the byproducts have similar sizes, sequences, and charges to the desired ssRNA ...
Nathaniel E. Clark +8 more
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High-efficiency purification of divergent AAV serotypes using AAVX affinity chromatography [PDF]
Molecular Therapy: Methods & Clinical Development, 2023 The adeno-associated viral vector (AAV) provides a safe and efficient gene therapy platform with several approved products that have marked therapeutic impact for patients.
Michael Florea +8 more
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Kinetic Analysis by Affinity Chromatography [PDF]
Frontiers in Chemistry, 2019 Important information on chemical processes in living systems can be obtained by the rates at which these biological interactions occur. This review will discuss several techniques based on traditional and high-performance affinity chromatography that ...
Sazia Iftekhar +2 more
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Extending the Affinity Range of Weak Affinity Chromatography for the Identification of Weak Ligands Targeting Membrane Proteins [PDF]
Molecules, 2023 The identification of weak-affinity ligands targeting membrane proteins is of great interest in Fragment-Based Drug Design (FBDD). Recently, miniaturized weak affinity chromatography (WAC) has been proposed as a valuable tool to study interactions ...
Adrien Deloche +5 more
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Ligand Screening of Membrane Proteins Embedded in Nanodiscs: How to Manage Non-Specific Interactions in Weak Affinity Chromatography? [PDF]
MoleculesMiniaturized weak affinity chromatography is emerging as an interesting alternative to conventional biophysical tools for performing fragment-screening studies in the context of fragment-based drug discovery.
François-Xavier Vidal +6 more
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Snorkel‐tag based affinity chromatography for recombinant extracellular vesicle purification [PDF]
Journal of Extracellular VesiclesExtracellular vesicles (EVs) are lipid nanoparticles and play an important role in cell‐cell communications, making them potential therapeutic agents and allowing to engineer for targeted drug delivery.
Madhusudhan Reddy Bobbili +16 more
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High-grade extracellular vesicles preparation by combined size-exclusion and affinity chromatography [PDF]
Scientific Reports, 2021 Extracellular vesicles (EVs) have recently gained growing interest for their diagnostic and therapeutic potential. Despite this, few protocols have been reported for the isolation of EVs with preserved biological function.
Cristina Bellotti +4 more
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Membranes, 2023 Several chromatographic approaches have been established over the last decades for the production of pharmaceutically relevant viruses. Due to the large size of these products compared to other biopharmaceuticals, e.g., proteins, convective flow media ...
Keven Lothert, Michael W. Wolff
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Lectin Purification through Affinity Chromatography Exploiting Macroporous Monolithic Adsorbents
Separations, 2023 Growing medical, engineering, biochemical, and biological interest has led to a steady pace of research and development into polymeric monolithic structures with densely interconnected pores for purifying bio compounds.
Josiane F. da Silva +14 more
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Bio-Protocol, 2013 RNA-affinity chromatography assays are used to identify proteins binding specific RNA sequences. These proteins represent potential factors contributing to the function of RNA molecules. In our lab, we have used this protocol to identify proteins binding
Lucia Morales +3 more
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