Results 41 to 50 of about 539,588 (346)

Antibody Fragments and Their Purification by Protein L Affinity Chromatography

Antibodies, 2015
Antibodies and related proteins comprise one of the largest and fastest-growing classes of protein pharmaceuticals. A majority of such molecules are monoclonal antibodies; however, many new entities are antibody fragments.
Gustav Rodrigo, Mats Gruvegård, James M. Van Alstine   +2 more
doaj   +1 more source

Surface Mutation Thr34His Facilitates Purification of Haemophilus influenza Carbonic Anhydrase via Metal Affinity Chromatography [PDF]

, 2017
In order to pursue Haemophilus influenza carbonic anhydrase (HICA) as a potential drug target, easy and efficient purification methods must be developed. While immobilized metal affinity chromatography (IMAC) may be used, complications with polyhistidine
Cornely, Dr. Kathleen, Foley, Caroline
core   +1 more source

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

FEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

Analysis of free analyte fractions by rapid affinity chromatography [PDF]

, 2004
The invention is generally directed toward an analytical method to determine the concentration of the free analyte fraction in a sample. More particularly, the method encompasses applying a sample comprising a free and bound analyte fraction to an ...
Clarke, William A., Hage, David S.
core   +1 more source

Liquid chromatographic strategies for separation of bioactive compounds in food matrices [PDF]

, 2018
Nowadays, there is an increasing attention for nutraceuticals and, in general, bioactive compounds naturally present in food. Indeed, the possibility of preserving human health and preventing disease (e.g., cardiovascular diseases, cancer etc.) by the ...
Capriotti, Anna Laura, Cavaliere, Chiara, La Barbera, Giorgia, Laganà, Aldo, Montone, Carmela Maria, Piovesana, Susy   +5 more
core   +1 more source

Structural insights into lacto‐N‐biose I recognition by a family 32 carbohydrate‐binding module from Bifidobacterium bifidum

FEBS Letters, EarlyView.
Bifidobacterium bifidum establishes symbiosis with infants by metabolizing lacto‐N‐biose I (LNB) from human milk oligosaccharides (HMOs). The extracellular multidomain enzyme LnbB drives this process, releasing LNB via its catalytic glycoside hydrolase family 20 (GH20) lacto‐N‐biosidase domain.
Xinzhe Zhang, Naoki Sunagawa, Toma Kashima, Kiyohiko Igarashi, Akimasa Miyanaga, Shinya Fushinobu   +5 more
wiley   +1 more source

Novel prokaryotic expression of thioredoxin-fused insulinoma associated protein tyrosine phosphatase 2 (IA-2), its characterization and immunodiagnostic application [PDF]

, 2016
Background The insulinoma associated protein tyrosine phosphatase 2 (IA-2) is one of the immunodominant autoantigens involved in the autoimmune attack to the beta-cell in Type 1 Diabetes Mellitus.
A Trabucchi, A Trabucchi, A Trabucchi, A Villalba, A Villalba, A Westerlund-Karlsson, Adriana Victoria Sabljic, AG Krochik, AL Notkins, Aldana Trabucchi, BD Spangler, Bruno David Rovitto, C Levy-Marchal, C Tiberti, C Torn, CA Baer, CC Richardson, CF Verge, D Barford, DU Rabin, DU Rabin, E Bonifacio, E Bonifacio, E Kawasaki, EA Gale, Edgardo Poskus, ELD Harlow, ER LaVallie, ER LaVallie, ET Mambetisaeva, Expert Committee on the Diagnosis and Classification of Diabetes Mellitus, F Baneyx, F Sodoyez-Goffaux, G Hannig, GS Eisenbarth, H Goetz, H Mziaut, H Mziaut, H Shägger, H Xie, I Kikkas, J Sambrook, J Seissler, J Suckale, JA Berzofsky, JA Berzofsky, JA Dromey, JC Sodoyez, JM Gonzalez-Buitrago, JM Wenzlau, JP Palmer, K Lobner, KT Elvers, L Castano, LL Guerra, Luciano Lucas Guerra, M Hawa, M Knip, M Masuda, M Solimena, M Trajkovski, M Trajkovski, MA Payton, ML Papouchado, ML Papouchado, MP Sica, MS Lan, MS Lan, Natalia Inés Faccinetti, NG Morgenthaler, O Pines, P Tompa, PD Burbelo, PJ Bingley, PZ Zimmet, R Gianani, R Turner, RD Hoffman, RR Stumpo, Ruben Francisco Iacono, S Chen, S Krause, S Schubert, S Torii, Silvina Noemí Valdez, SJ Kim, SLBP Thrower, SN Valdez, SN Valdez, T Ort, T Tuomi, VN Uversky, WA Hagopian, X Jia, X Palomer, Z Zhao   +95 more
core   +5 more sources

Peptide‐based ligand antagonists block a Vibrio cholerae adhesin

FEBS Letters, EarlyView.
The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang, Grace Du, Charity Yongo‐Luwawa, Angelina Lu, Brett Kinrade, Kim Munro, Karl E. Klose, William D. Lubell, Peter Davies, Shuaiqi Guo   +9 more
wiley   +1 more source

Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions. [PDF]

PLoS ONE, 2008
We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on ...
Roberta Schulte, Jessica Talamas, Christine Doucet, Martin W Hetzer   +3 more
doaj   +1 more source

Identification of microtubule-associated proteins in the centrosome, spindle, and kinetochore of the early Drosophila embryo. [PDF]

, 1989
We have developed affinity chromatography methods for the isolation of microtubule-associated proteins (MAPs) from soluble cytoplasmic extracts and have used them to analyze the cytoskeleton of the early Drosophila embryo.
Alberts, BM, Field, CM, Kellogg, DR
core