Results 41 to 50 of about 301,127 (155)

Reaction of Native and Denatured Brucella abortus (S19) Proteins with Antibody Using Affinity Chromatography and Immunoblotting

پزشکی بالینی ابن سینا, 2005
Western blotting or immunoblotting commonly use for study of reaction between antigens and antibodies. Denaturation of many proteins in immunoblotting can affect greatly the reactivity of antibodies and outcome of the procedure.
Ramin Karimi, Ali Mostafaie, Bahman Tabaraie, Yadollah Bahrami, Jalal Abdolalizadeh   +4 more
doaj  

Feasibility of preparing human plasminogen by full chromatography from precipitation of Fraction Ⅲ in low temperature ethanol method

Zhongguo shuxue zazhi
[Objective] To determine the feasibility of preparing plasminogen (Pg) with Fraction Ⅲ precipitation (hereinafter referred to as FⅢ-P) from low-temperature ethanol process by full chromatography (hereinafter referred to as FⅢ-P process). [Methods] The FⅢ-
ZHANG Jin, YUE Shenglan, ZHU Chen, PENG Yan, ZHOU Yanxiang, LIN Lianzhen, CHEN Kejin, FENG Lu, HU Yong, ZHOU Zhijun   +9 more
doaj   +1 more source

A New Affinity Gel Synthesized for Phenylalanine Ammonia Lyase Isolated from Red Clover (Trifolium pratense L.) Leaf and an Investigation into Its Kinetic Properties

Separations
Phenylalanine ammonia lyase (PAL) was first purified using affinity chromatography from the leaves of red-flowered clover, a highly antioxidant source.
Yavuz Selim Toksöz, Çiğdem Bilen, Emine Karakuş   +2 more
doaj   +1 more source

Evaluation of Caesalpinia pulcherrima endospermic gum as affinity matrices for galactose-binding lectins interaction

Brazilian Archives of Biology and Technology, 2011
Lectins are proteins or glycoproteins able to bind, specifically and reversibly carbohydrates and glycoconjugates. Considering this ability, the utilization of Caesalpinia pulcherrima seeds polysaccharides as an affinity matrix was tested.
Renata Chastinet Braga, Daniele Maria Alves Teixeira-Sá, Alexsandra Feitosa Ribeiro, Raquel Lima Miranda, Lia Magalhães de Almeida, Ana Cecília Góes Horta, Renato de Azevedo Moreira   +6 more
doaj   +1 more source

Characterization of the galactomannans from Parkinsonia aculeata seeds and their application on affinity chromatography

Polímeros, 2006
Successive aqueous (10 ºC, 25 ºC and 80 ºC) and alkaline (1M NaOH; 25 ºC) extractions of milled Parkinsonia aculeata endosperms gave rise to four galactomannan fractions. These extractions furnished viscous galactomannans with Man:Gal ratios ranging from
Ivone Garros-Rosa, Fany Reicher, Carmen L. O. Petkowicz, Maria Rita Sierakowski, Renato A. Moreira   +4 more
doaj   +1 more source

Solid-Supported Proteins in the Liquid Chromatography Domain to Probe Ligand-Target Interactions

Frontiers in Chemistry, 2019
Ligand-target interactions play a central role in drug discovery processes because these interactions are crucial in biological systems. Small molecules-proteins interactions can regulate and modulate protein function and activity through conformational ...
Marcela Cristina de Moraes, Carmen Lucia Cardoso, Quezia Bezerra Cass   +2 more
doaj   +1 more source

Purification of a Protein Exhibiting Isoleucine 2-epimerase Activity from Lactobacillus otakiensis JCM 15040

Bio-Protocol, 2015
Prominent accumulation of D-leucine, D-allo-isoleucine and D-valine was observed in the culture medium of the heterofermentative bacterial species, Lactobacillus otakiensis (L. otakiensis) JCM 15040. The racemase enzyme that resulted in this accumulation,
Yuta Mutaguchi, Toshihisa Ohshima
doaj   +1 more source

A streamlined tandem affinity purification of His-MBP-SpyCas9, without buffer exchange, suitable for in vitro cleavage applications

MethodsX
We present a streamlined protocol for the purification of recombinant doubly-tagged His-MBP-SpyCas9 protein utilizing a dual affinity chromatography approach whereby the elution volume from immobilized metal ion affinity chromatography (IMAC) is loaded ...
Filippo Fronza, Roberto Verardo, Claudio Schneider   +2 more
doaj   +1 more source

Comprehensive Comparison of AAV Purification Methods: Iodixanol Gradient Centrifugation vs. Immuno-Affinity Chromatography. [PDF]

Adv Cell Gene Ther, 2023
Lam AK, Mulcrone PL, Frabutt D, Zhang J, Chrzanowski M, Arisa S, Munoz M, Li X, Biswas M, Markusic D, Herzog RW, Xiao W.   +11 more
europepmc   +1 more source

Optimization of Affinity Chromatography Based on Sepharose 4B- chitin for Rapid Purification of Urtica dioica Agglutinin. [PDF]

Iran J Biotechnol, 2023
Heydari M, Hosseinzadeh Colagar A, Sabour D.   +2 more
europepmc   +1 more source