PVA-Glutaraldehyde as support for lectin immobilization and affinity chromatography
Acta Scientiarum: Biological Sciences, 2016 Immobilized lectins are a powerful biotechnological tool for separation and isolation of glycoconjugates. In the present study, polyvinyl alcohol (PVA) and glutaraldehyde (GA) were used as a support for Concanavalin A (Con A) covalent immobilization and ...
Moacyr Jesus Barreto de Melo Rêgo +5 more
doaj +1 more source
Amphiphilic diblock copolymers as functional surfaces for protein chromatography [PDF]
, 2022 Stationary phase plays a crucial role in the operation of a protein chromatography column. Conventional resins composed of acrylic polymers and their derivatives contribute to heterogeneity of the packing of stationary phase inside these columns. Alternative polymer combinations through customized surface functionalization schemes which consist of ...
arxiv +1 more source
Chimeric protein complexes in hybrid species generate novel evolutionary phenotypes [PDF]
PLoS Genet. 2013 Oct;9(10):e1003836, 2012 Hybridization between species is an important mechanism for the origin of novel lineages and adaptation to new environments. Increased allelic variation and modification of the transcriptional network are the two recognized forces currently deemed to be responsible for the phenotypic properties seen in hybrids.
arxiv +1 more source
Purification of phage display-modified bacteriophage T4 by affinity chromatography
BMC Biotechnology, 2011 Background Affinity chromatography is one of the most efficient protein purification strategies. This technique comprises a one-step procedure with a purification level in the order of several thousand-fold, adaptable for various proteins, differentiated
Figura Grzegorz +6 more
doaj +1 more source
Separation Options for Phosphorylated Osteopontin from Transgenic Microalgae Chlamydomonas reinhardtii. [PDF]
, 2018 Correct folding and post-translational modifications are vital for therapeutic proteins to elicit their biological functions. Osteopontin (OPN), a bone regenerative protein present in a range of mammalian cells, is an acidic phosphoprotein with multiple ...
Guo, Shengchun +5 more
core +2 more sources
Frontiers in Endocrinology, 2015 Affinity chromatography is an efficient method to isolate proteins by taking advantage of their affinities for specific molecules such as substrates, inhibitors, antigens, ligands, antibodies, and other interacting molecules including subunits. Nowadays,
Yoko eFujita-Yamaguchi
doaj +1 more source
Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions. [PDF]
PLoS ONE, 2008 We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on ...
Roberta Schulte +3 more
doaj +1 more source
Surface Mutation Thr34His Facilitates Purification of Haemophilus influenza Carbonic Anhydrase via Metal Affinity Chromatography [PDF]
, 2017 In order to pursue Haemophilus influenza carbonic anhydrase (HICA) as a potential drug target, easy and efficient purification methods must be developed. While immobilized metal affinity chromatography (IMAC) may be used, complications with polyhistidine
Cornely, Dr. Kathleen, Foley, Caroline
core +1 more source
Identification of novel small molecule inhibitors of ETS transcription factors
FEBS Letters, EarlyView.ETS transcription factors play an essential role in tumourigenesis and are indispensable for sprouting angiogenesis, a hallmark of cancer, which fuels tumour expansion and dissemination. Thus, targeting ETS transcription factor function could represent an effective, multifaceted strategy to block tumour growth. The evolutionarily conserved E‐Twenty‐Six
Shaima Abdalla +9 more
wiley +1 more source
A high-affinity antibody against the CSP N-terminal domain lacks Plasmodium falciparum inhibitory activity [PDF]
, 2020 Malaria is a global health concern and research efforts are ongoing to develop a superior vaccine to RTS,S/AS01. To guide immunogen design, we seek a comprehensive understanding of the protective humoral response against Plasmodium falciparum ...
Bosch, A. +15 more
core +2 more sources