Results 71 to 80 of about 329,197 (260)

Systematic Selection of High‐Affinity ssDNA Sequences to Carbon Nanotubes

Advanced Science
Single‐walled carbon nanotubes (SWCNTs) have gained significant interest for their potential in biomedicine and nanoelectronics. The functionalization of SWCNTs with single‐stranded DNA (ssDNA) enables the precise control of SWCNT alignment and the ...
Dakyeon Lee, Jaekang Lee, Woojin Kim, Yeongjoo Suh, Jiwoo Park, Sungjee Kim, YongJoo Kim, Sunyoung Kwon, Sanghwa Jeong   +8 more
doaj   +1 more source

ADP‐ribosylation: An emerging regulator of the epigenome

Molecular Oncology, EarlyView.
ADP‐ribosylation has emerged as a dynamic epigenetic signaling mechanism that modifies histones and chromatin‐associated proteins. Through coordinated PARylation and MARylation, it integrates with other histone modifications to regulate chromatin structure, transcription factor activity, and gene expression, influencing genome function and disease ...
Cristel V. Camacho, Chenqian Liu, W. Lee Kraus   +2 more
wiley   +1 more source

DeepAptamer: Advancing high-affinity aptamer discovery with a hybrid deep learning model

Molecular Therapy: Nucleic Acids
Oligonucleotide aptamers are typically identified through a rigorous and time-consuming process known as systematic evolution of ligands by exponential enrichment (SELEX), which requires 20 to 30 iterative rounds to eliminate non/weak binding sequences ...
Xin Yang, Chi Ho Chan, Shanshan Yao, Hang Yin Chu, Minchuan Lyu, Ziqi Chen, Huan Xiao, Yuan Ma, Sifan Yu, Fangfei Li, Jin Liu, Luyao Wang, Zongkang Zhang, Bao-Ting Zhang, Lu Zhang, Aiping Lu, Yaofeng Wang, Ge Zhang, Yuanyuan Yu   +18 more
doaj   +1 more source

Peptide inhibitor of pancreatic lipase selected by phage display using different elution strategies

Journal of Lipid Research, 2005
Interference with fat hydrolysis results in the reduced use of ingested lipids. Inhibition of pancreatic lipase reduces the efficiency of fat absorption in the small intestine and thereby initiates modest long-term reduction in body weight. In an attempt
M. Lunder, T. Bratkovič, S. Kreft, B. Štrukelj   +3 more
doaj   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Engineering of bispecific affinity proteins with high affinity for ERBB2 and adaptable binding to albumin.

PLoS ONE, 2014
The epidermal growth factor receptor 2, ERBB2, is a well-validated target for cancer diagnostics and therapy. Recent studies suggest that the over-expression of this receptor in various cancers might also be exploited for antibody-based payload delivery,
Johan Nilvebrant, Mikael Åstrand, Maria Georgieva-Kotseva, Mattias Björnmalm, John Löfblom, Sophia Hober   +5 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Asthma prediction via affinity graph enhanced classifier: a machine learning approach based on routine blood biomarkers

Journal of Translational Medicine
Background Asthma is a chronic respiratory disease affecting millions of people worldwide, but early detection can be challenging due to the time-consuming nature of the traditional technique.
Dejing Li, Stanley Ebhohimhen Abhadiomhen, Dongmei Zhou, Xiang-Jun Shen, Lei Shi, Yubao Cui   +5 more
doaj   +1 more source

Affinity as a tool in life science

BioTechniques, 2008
The use of affinity-based tools has become invaluable as a platform for basic research and in the development of drugs and diagnostics. Applications include affinity chromatography and affinity tag fusions for efficient purification of proteins as well ...
Mathias Uhlén
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source