Results 61 to 70 of about 738,160 (293)
One-step selection of Vaccinia virus-binding DNA aptamers by MonoLEX
BMC Biotechnology, 2007 Background As a new class of therapeutic and diagnostic reagents, more than fifteen years ago RNA and DNA aptamers were identified as binding molecules to numerous small compounds, proteins and rarely even to complete pathogen particles.
Stöcklein Walter +11 more
doaj +1 more source
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Probing the limits of aptamer affinity with a microfluidic SELEX platform. [PDF]
PLoS ONE, 2011 Nucleic acid-based aptamers offer many potential advantages relative to antibodies and other protein-based affinity reagents, including facile chemical synthesis, reversible folding, improved thermal stability and lower cost.
Kareem M Ahmad +5 more
doaj +1 more source
Predicting B Cell Receptor Substitution Profiles Using Public Repertoire Data
, 2018 B cells develop high affinity receptors during the course of affinity maturation, a cyclic process of mutation and selection. At the end of affinity maturation, a number of cells sharing the same ancestor (i.e.
Davidsen, Kristian +3 more
core +2 more sources
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
Conformational Dynamics of the Receptor-Binding Domain of the SARS-CoV-2 Spike Protein
Biomedicines, 2022 Variants of SARS-CoV-2 keep emerging and causing new waves of COVID-19 around the world. Effective new approaches in drug development are based on the binding of agents, such as neutralizing monoclonal antibodies to a receptor-binding domain (RBD) of ...
Aleksandra A. Mamchur +8 more
doaj +1 more source
On Affine Selections of Set–Valued Functions [PDF]
JAA, 1995 Abstract The main result of this paper is the theorem stating that every convex set–valued function F : I ↦ c(Y), where I ⊂ R is an interval and Y is a locally convex space, possesses an affine selection. In the case if Y = R and values of F are closed real intervals we can replace the assumption of convexity of F by the more general ...
openaire +3 more sources
AAA+ protein unfoldases—the Moirai of the proteome
FEBS Letters, EarlyView.AAA+ unfoldases are essential molecular motors that power protein degradation and disaggregation. This review integrates recent cryo‐electron microscopy (cryo‐EM) structures and single‐molecule biophysical data to reconcile competing models of substrate translocation.
Stavros Azinas, Marta Carroni
wiley +1 more source
Development of Recombinant Antibody by Yeast Surface Display Technology
Current Research in Pharmacology and Drug DiscoveryRecombinant antibodies have emerged as powerful tools in various fields, including therapeutics, diagnostics, and research applications. The selection of high-affinity antibodies with desired specificity is a crucial step in the development of ...
Mujahed I. Mustafa +2 more
doaj +1 more source
Parameters and determinants of responses to selection in antibody libraries.
PLoS Computational Biology, 2021 The sequences of antibodies from a given repertoire are highly diverse at few sites located on the surface of a genome-encoded larger scaffold. The scaffold is often considered to play a lesser role than highly diverse, non-genome-encoded sites in ...
Steven Schulz +6 more
doaj +1 more source