Results 11 to 20 of about 176 (87)

Tuning a high performing multiplexed-CRISPRi Pseudomonas putida strain to further enhance indigoidine production [PDF]

Metabolic Engineering Communications, 2022
In this study, a 14-gene edited Pseudomonas putida KT2440 strain for heterologous indigoidine production was examined using three distinct omic datasets. Transcriptomic data indicated that CRISPR/dCpf1-interference (CRISPRi) mediated multiplex repression
Jeffrey J. Czajka, Deepanwita Banerjee, Thomas Eng, Javier Menasalvas, Chunsheng Yan, Nathalie Munoz Munoz, Brenton C. Poirier, Young-Mo Kim, Scott E. Baker, Yinjie J. Tang, Aindrila Mukhopadhyay   +10 more
doaj   +2 more sources

CRISPR-Cas9/Cas12a systems for efficient genome editing and large genomic fragment deletions in Aspergillus niger [PDF]

Frontiers in Bioengineering and Biotechnology
CRISPR technology has revolutionized fungal genetic engineering by accelerating the pace and expanding the feasible scope of experiments in this field. Among various CRISPR-Cas systems, Cas9 and Cas12a are widely used in genetic and metabolic engineering.
Guoliang Yuan, Guoliang Yuan, Shuang Deng, Shuang Deng, Jeffrey J. Czajka, Jeffrey J. Czajka, Ziyu Dai, Ziyu Dai, Beth A. Hofstad, Beth A. Hofstad, Joonhoon Kim, Joonhoon Kim, Kyle R. Pomraning, Kyle R. Pomraning   +13 more
doaj   +2 more sources

Machine learning-led semi-automated medium optimization reveals salt as key for flaviolin production in Pseudomonas putida [PDF]

Communications Biology
Although synthetic biology can produce valuable chemicals in a renewable manner, its progress is still hindered by a lack of predictive capabilities. Media optimization is a critical, and often overlooked, process which is essential to obtain the titers,
Apostolos Zournas, Matthew R. Incha, Tijana Radivojevic, Vincent Blay, Jose Manuel Martí, Zak Costello, Matthias Schmidt, Tan Chung, Mitchell G. Thompson, Allison Pearson, Patrick C. Kinnunen, Thomas Eng, Christopher E. Lawson, Stephen Tan, Tadeusz Ogorzalek, Nurgul Kaplan, Mark Forrer, Tyler Backman, Aindrila Mukhopadhyay, Nathan J. Hillson, Jay D. Keasling, Hector Garcia Martin   +21 more
doaj   +2 more sources

Genome-scale model development and genomic sequencing of the oleaginous clade Lipomyces [PDF]

Frontiers in Bioengineering and Biotechnology
The Lipomyces clade contains oleaginous yeast species with advantageous metabolic features for biochemical and biofuel production. Limited knowledge about the metabolic networks of the species and limited tools for genetic engineering have led to a ...
Jeffrey J. Czajka, Jeffrey J. Czajka, Yichao Han, Yichao Han, Joonhoon Kim, Joonhoon Kim, Joonhoon Kim, Stephen J. Mondo, Stephen J. Mondo, Stephen J. Mondo, Beth A. Hofstad, Beth A. Hofstad, AnaLaura Robles, AnaLaura Robles, Sajeet Haridas, Robert Riley, Kurt LaButti, Jasmyn Pangilinan, William Andreopoulos, Anna Lipzen, Juying Yan, Mei Wang, Vivian Ng, Igor V. Grigoriev, Igor V. Grigoriev, Joseph W. Spatafora, Jon K. Magnuson, Jon K. Magnuson, Jon K. Magnuson, Scott E. Baker, Scott E. Baker, Scott E. Baker, Kyle R. Pomraning, Kyle R. Pomraning   +33 more
doaj   +2 more sources

A Procedural Framework for Benchmarking Biofoundry Capabilities [PDF]

ACS Synthetic Biology, 2023
Nathan Hillson
exaly   +2 more sources

Building a global alliance of biofoundries [PDF]

Nature Communications, 2019
Biofoundries provide an integrated infrastructure to enable the rapid design, construction, and testing of genetically reprogrammed organisms for biotechnology applications and research.
Nathan Hillson, Mark Caddick, Yizhi Cai, Jose A. Carrasco, Matthew Wook Chang, Natalie C. Curach, David J. Bell, Rosalind Le Feuvre, Douglas C. Friedman, Xiongfei Fu, Nicholas D. Gold, Markus J. Herrgård, Maciej B. Holowko, James R. Johnson, Richard A. Johnson, Jay D. Keasling, Richard I. Kitney, Akihiko Kondo, Chenli Liu, Vincent J. J. Martin, Filippo Menolascina, Chiaki Ogino, Nicola J. Patron, Marilene Pavan, Chueh Loo Poh, Isak S. Pretorius, Susan J. Rosser, Nigel S. Scrutton, Marko Storch, Hille Tekotte, Evelyn Travnik, Claudia E. Vickers, Wen Shan Yew, Yingjin Yuan, Huimin Zhao, Paul S. Freemont   +35 more
doaj   +2 more sources

Editorial: Multi-Omics Technologies for Optimizing Synthetic Biomanufacturing [PDF]

Frontiers in Bioengineering and Biotechnology, 2021
Young-Mo Kim, Young-Mo Kim, Christopher J. Petzold, Christopher J. Petzold, Eduard J. Kerkhoven, Scott E. Baker, Scott E. Baker   +6 more
doaj   +2 more sources

Author Correction: Machine learning-led semi-automated medium optimization reveals salt as key for flaviolin production in Pseudomonas putida [PDF]

Communications Biology
Apostolos Zournas, Matthew R. Incha, Tijana Radivojevic, Vincent Blay, Jose Manuel Martí, Zak Costello, Matthias Schmidt, Tan Chung, Mitchell G. Thompson, Allison Pearson, Patrick C. Kinnunen, Thomas Eng, Christopher E. Lawson, Stephen Tan, Tadeusz Ogorzalek, Nurgul Kaplan, Mark Forrer, Tyler Backman, Aindrila Mukhopadhyay, Nathan J. Hillson, Jay D. Keasling, Hector Garcia Martin   +21 more
doaj   +2 more sources

Addressing the post‐COVID era through engineering biology

Engineering Biology, Volume 5, Issue 2, Page 21-34, June 2021., 2021
Abstract Currently, the world is faced with two fundamental issues of great importance, namely climate change and the coronavirus pandemic. These are intimately involved with the need to control climate change and the need to switch from high carbon, unsustainable economies to low carbon economies.
Jennifer Bell, Jim Philp, Richard I. Kitney   +2 more
wiley   +1 more source

Future trends in synthetic biology in Asia

Advanced Genetics, Volume 2, Issue 1, March 2021., 2021
Abstract Synthetic biology research and technology translation has garnered increasing interest from the governments and private investors in Asia, where the technology has great potential in driving a sustainable bio‐based economy. This Perspective reviews the latest developments in the key enabling technologies of synthetic biology and its ...
Ning Mao, Nikhil Aggarwal, Chueh Loo Poh, Byung Kwan Cho, Akihiko Kondo, Chenli Liu, Wen Shan Yew, Matthew Wook Chang   +7 more
wiley   +1 more source