Results 21 to 30 of about 176 (87)

Multi-Omics Driven Metabolic Network Reconstruction and Analysis of Lignocellulosic Carbon Utilization in Rhodosporidium toruloides

open access: yesFrontiers in Bioengineering and Biotechnology, 2021
An oleaginous yeast Rhodosporidium toruloides is a promising host for converting lignocellulosic biomass to bioproducts and biofuels. In this work, we performed multi-omics analysis of lignocellulosic carbon utilization in R. toruloides and reconstructed
Joonhoon Kim   +30 more
doaj   +1 more source

Targeted mutagenesis and high-throughput screening of diversified gene and promoter libraries for isolating gain-of-function mutations

open access: yesFrontiers in Bioengineering and Biotechnology, 2023
Targeted mutagenesis of a promoter or gene is essential for attaining new functions in microbial and protein engineering efforts. In the burgeoning field of synthetic biology, heterologous genes are expressed in new host organisms.
Herbert M. Huttanus   +16 more
doaj   +1 more source

Multiomics Data Collection, Visualization, and Utilization for Guiding Metabolic Engineering

open access: yesFrontiers in Bioengineering and Biotechnology, 2021
Biology has changed radically in the past two decades, growing from a purely descriptive science into also a design science. The availability of tools that enable the precise modification of cells, as well as the ability to collect large amounts of ...
Somtirtha Roy   +28 more
doaj   +1 more source

High-Throughput Large-Scale Targeted Proteomics Assays for Quantifying Pathway Proteins in Pseudomonas putida KT2440

open access: yesFrontiers in Bioengineering and Biotechnology, 2020
Targeted proteomics is a mass spectrometry-based protein quantification technique with high sensitivity, accuracy, and reproducibility. As a key component in the multi-omics toolbox of systems biology, targeted liquid chromatography-selected reaction ...
Yuqian Gao   +40 more
doaj   +1 more source

A machine learning Automated Recommendation Tool for synthetic biology

open access: yesNature Communications, 2020
Synthetic Biology often lacks the predictive power needed for efficient bioengineering. Here the authors present ART, a machine learning and probabilistic predictive tool to guide synthetic biology design in a systematic fashion.
Tijana Radivojević   +3 more
doaj   +1 more source

Omics-Driven Biotechnology for Industrial Applications

open access: yesFrontiers in Bioengineering and Biotechnology, 2021
Biomanufacturing is a key component of biotechnology that uses biological systems to produce bioproducts of commercial relevance, which are of great interest to the energy, material, pharmaceutical, food, and agriculture industries.
Bashar Amer   +4 more
doaj   +1 more source

Visualizing the invisible: class excursions to ignite children’s enthusiasm for microbes

open access: yesMicrobial Biotechnology, Volume 13, Issue 4, Page 844-887, July 2020., 2020
The ubiquity of microbes, their manifold activities and pervasive influence on the health of all life, local environments and the planet, necessitate an understanding of relevant microbial processes for informed, evidence‐based decision‐making at all levels of society – i.e. Microbiology Literacy.
Terry J. McGenity   +30 more
wiley   +1 more source

Exploiting nonionic surfactants to enhance fatty alcohol production in Rhodosporidium toruloides

open access: yesBiotechnology and Bioengineering, Volume 117, Issue 5, Page 1418-1425, May 2020., 2020
Microbial production of fatty alcohols has gained broad interests, however one major challenge for commercialization of this technology is inhibition of cell growth caused by intracellular fatty alcohol accumulation. This study develops a novel strategy that exploits nonionic surfactants to promote the export of fatty alcohols from R.
Di Liu   +10 more
wiley   +1 more source

Integration of Proteomics and Metabolomics Into the Design, Build, Test, Learn Cycle to Improve 3-Hydroxypropionic Acid Production in Aspergillus pseudoterreus

open access: yesFrontiers in Bioengineering and Biotechnology, 2021
Biological engineering of microorganisms to produce value-added chemicals is a promising route to sustainable manufacturing. However, overproduction of metabolic intermediates at high titer, rate, and yield from inexpensive substrates is challenging in ...
Kyle R. Pomraning   +21 more
doaj   +1 more source

Production of ent-kaurene from lignocellulosic hydrolysate in Rhodosporidium toruloides

open access: yesMicrobial Cell Factories, 2020
Background Rhodosporidium toruloides has emerged as a promising host for the production of bioproducts from lignocellulose, in part due to its ability to grow on lignocellulosic feedstocks, tolerate growth inhibitors, and co-utilize sugars and lignin ...
Gina M. Geiselman   +15 more
doaj   +1 more source

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