Results 21 to 30 of about 176 (87)
Multi-Omics Driven Metabolic Network Reconstruction and Analysis of Lignocellulosic Carbon Utilization in Rhodosporidium toruloides
Frontiers in Bioengineering and Biotechnology, 2021 An oleaginous yeast Rhodosporidium toruloides is a promising host for converting lignocellulosic biomass to bioproducts and biofuels. In this work, we performed multi-omics analysis of lignocellulosic carbon utilization in R. toruloides and reconstructed Joonhoon Kim, Joonhoon Kim, Joonhoon Kim, Samuel T. Coradetti, Samuel T. Coradetti, Young-Mo Kim, Young-Mo Kim, Yuqian Gao, Yuqian Gao, Junko Yaegashi, Junko Yaegashi, Jeremy D. Zucker, Jeremy D. Zucker, Nathalie Munoz, Nathalie Munoz, Erika M. Zink, Kristin E. Burnum-Johnson, Kristin E. Burnum-Johnson, Scott E. Baker, Scott E. Baker, Scott E. Baker, Blake A. Simmons, Blake A. Simmons, Blake A. Simmons, Jeffrey M. Skerker, John M. Gladden, John M. Gladden, John M. Gladden, Jon K. Magnuson, Jon K. Magnuson, Jon K. Magnuson +30 moredoaj +1 more sourceTargeted mutagenesis and high-throughput screening of diversified gene and promoter libraries for isolating gain-of-function mutations
Frontiers in Bioengineering and Biotechnology, 2023 Targeted mutagenesis of a promoter or gene is essential for attaining new functions in microbial and protein engineering efforts. In the burgeoning field of synthetic biology, heterologous genes are expressed in new host organisms.Herbert M. Huttanus, Herbert M. Huttanus, Ellin-Kristina H. Triola, Ellin-Kristina H. Triola, Jeanette C. Velasquez-Guzman, Jeanette C. Velasquez-Guzman, Sang-Min Shin, Sang-Min Shin, Rommel S. Granja-Travez, Rommel S. Granja-Travez, Anmoldeep Singh, Taraka Dale, Taraka Dale, Taraka Dale, Ramesh K. Jha, Ramesh K. Jha, Ramesh K. Jha +16 moredoaj +1 more sourceMultiomics Data Collection, Visualization, and Utilization for Guiding Metabolic Engineering
Frontiers in Bioengineering and Biotechnology, 2021 Biology has changed radically in the past two decades, growing from a purely descriptive science into also a design science. The availability of tools that enable the precise modification of cells, as well as the ability to collect large amounts of ...Somtirtha Roy, Somtirtha Roy, Tijana Radivojevic, Tijana Radivojevic, Tijana Radivojevic, Mark Forrer, Mark Forrer, Mark Forrer, Jose Manuel Marti, Jose Manuel Marti, Jose Manuel Marti, Vamshi Jonnalagadda, Vamshi Jonnalagadda, Tyler Backman, Tyler Backman, William Morrell, William Morrell, William Morrell, Hector Plahar, Hector Plahar, Joonhoon Kim, Joonhoon Kim, Nathan Hillson, Nathan Hillson, Nathan Hillson, Hector Garcia Martin, Hector Garcia Martin, Hector Garcia Martin, Hector Garcia Martin +28 moredoaj +1 more sourceHigh-Throughput Large-Scale Targeted Proteomics Assays for Quantifying Pathway Proteins in Pseudomonas putida KT2440
Frontiers in Bioengineering and Biotechnology, 2020 Targeted proteomics is a mass spectrometry-based protein quantification technique with high sensitivity, accuracy, and reproducibility. As a key component in the multi-omics toolbox of systems biology, targeted liquid chromatography-selected reaction ...Yuqian Gao, Yuqian Gao, Thomas L. Fillmore, Nathalie Munoz, Nathalie Munoz, Gayle J. Bentley, Gayle J. Bentley, Christopher W. Johnson, Christopher W. Johnson, Joonhoon Kim, Joonhoon Kim, Jamie A. Meadows, Jamie A. Meadows, Jeremy D. Zucker, Jeremy D. Zucker, Meagan C. Burnet, Meagan C. Burnet, Anna K. Lipton, Anna K. Lipton, Aivett Bilbao, Aivett Bilbao, Daniel J. Orton, Young-Mo Kim, Young-Mo Kim, Ronald J. Moore, Errol W. Robinson, Errol W. Robinson, Scott E. Baker, Scott E. Baker, Bobbie-Jo M. Webb-Robertson, Bobbie-Jo M. Webb-Robertson, Adam M. Guss, Adam M. Guss, John M. Gladden, John M. Gladden, Gregg T. Beckham, Gregg T. Beckham, Jon K. Magnuson, Jon K. Magnuson, Kristin E. Burnum-Johnson, Kristin E. Burnum-Johnson +40 moredoaj +1 more sourceOmics-Driven Biotechnology for Industrial Applications
Frontiers in Bioengineering and Biotechnology, 2021 Biomanufacturing is a key component of biotechnology that uses biological systems to produce bioproducts of commercial relevance, which are of great interest to the energy, material, pharmaceutical, food, and agriculture industries.Bashar Amer, Bashar Amer, Edward E. K. Baidoo, Edward E. K. Baidoo, Edward E. K. Baidoo +4 moredoaj +1 more sourceVisualizing the invisible: class excursions to ignite children’s enthusiasm for microbes
Microbial Biotechnology, Volume 13, Issue 4, Page 844-887, July 2020., 2020 The ubiquity of microbes, their manifold activities and pervasive influence on the health of all life, local environments and the planet, necessitate an understanding of relevant microbial processes for informed, evidence‐based decision‐making at all levels of society – i.e. Microbiology Literacy.Terry J. McGenity, Amare Gessesse, John E. Hallsworth, Esther Garcia Cela, Carol Verheecke‐Vaessen, Fengping Wang, Max Chavarría, Max M. Haggblom, Søren Molin, Antoine Danchin, Eddy J. Smid, Cédric Lood, Charles S. Cockell, Corinne Whitby, Shuang‐Jiang Liu, Nancy P. Keller, Lisa Y. Stein, Seth R. Bordenstein, Rup Lal, Olga C. Nunes, Lone Gram, Brajesh K. Singh, Nicole S. Webster, Cindy Morris, Sharon Sivinski, Saskia Bindschedler, Pilar Junier, André Antunes, Bonnie K. Baxter, Paola Scavone, Kenneth Timmis +30 morewiley +1 more sourceExploiting nonionic surfactants to enhance fatty alcohol production in Rhodosporidium toruloides
Biotechnology and Bioengineering, Volume 117, Issue 5, Page 1418-1425, May 2020., 2020 Microbial production of fatty alcohols has gained broad interests, however one major challenge for commercialization of this technology is inhibition of cell growth caused by intracellular fatty alcohol accumulation. This study develops a novel strategy that exploits nonionic surfactants to promote the export of fatty alcohols from R.Di Liu, Gina M. Geiselman, Samuel Coradetti, Ya‐Fang Cheng, James Kirby, Jan‐Philip Prahl, Oslo Jacobson, Eric R. Sundstrom, Deepti Tanjore, Jeffrey M. Skerker, John Gladden +10 morewiley +1 more sourceIntegration of Proteomics and Metabolomics Into the Design, Build, Test, Learn Cycle to Improve 3-Hydroxypropionic Acid Production in Aspergillus pseudoterreus
Frontiers in Bioengineering and Biotechnology, 2021 Biological engineering of microorganisms to produce value-added chemicals is a promising route to sustainable manufacturing. However, overproduction of metabolic intermediates at high titer, rate, and yield from inexpensive substrates is challenging in ...Kyle R. Pomraning, Ziyu Dai, Nathalie Munoz, Young-Mo Kim, Yuqian Gao, Shuang Deng, Joonhoon Kim, Joonhoon Kim, Beth A. Hofstad, Marie S. Swita, Teresa Lemmon, James R. Collett, Ellen A. Panisko, Bobbie-Jo M. Webb-Robertson, Jeremy D. Zucker, Carrie D. Nicora, Henrique De Paoli, Scott E. Baker, Kristin E. Burnum-Johnson, Nathan J. Hillson, Jon K. Magnuson, Jon K. Magnuson +21 moredoaj +1 more sourceProduction of ent-kaurene from lignocellulosic hydrolysate in Rhodosporidium toruloides
Microbial Cell Factories, 2020 Background Rhodosporidium toruloides has emerged as a promising host for the production of bioproducts from lignocellulose, in part due to its ability to grow on lignocellulosic feedstocks, tolerate growth inhibitors, and co-utilize sugars and lignin ...Gina M. Geiselman, Xun Zhuang, James Kirby, Mary B. Tran-Gyamfi, Jan-Philip Prahl, Eric R. Sundstrom, Yuqian Gao, Nathalie Munoz Munoz, Carrie D. Nicora, Derek M. Clay, Gabriella Papa, Kristin E. Burnum-Johnson, Jon K. Magnuson, Deepti Tanjore, Jeffrey M. Skerker, John M. Gladden +15 moredoaj +1 more source