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Dual-gRNA CRISPR/Cas9 Deletion of CsDMR6 in Sweet Orange Supported by Improved In Vitro Regeneration. [PDF]
Plants (Basel)Sopalda S +5 more
europepmc +1 more source
Correction to 'A method for in-depth analysis of circular DNA virus populations by unambiguously profiling the low abundant virus variants and partial genomic components'. [PDF]
Nucleic Acids Reseuropepmc +1 more source
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Robust soybean leaf agroinfiltration
Plant Cell ReportsA robust agroinfiltration-mediated transient gene expression method for soybean leaves was developed. Plant genotype, developmental stage and leaf age, surfactant, and Agrobacterium culture conditions are important for successful agroinfiltration.
Vincent Pantalone +2 more
exaly +4 more sources
Plant Cell Reports, 2011 Grapevine is an economically important crop, and the recent completion of its genome makes it possible to study the function of specific genes through reverse genetics. However, the analysis of gene function by RNA interference (RNAi) in grapevine is difficult, because the generation of stable transgenic plants has low efficiency and is time consuming.
Nadia Bertazzon +2 more
exaly +7 more sources
A simple and efficient agroinfiltration method for transient gene expression in Citrus
Plant Cell Reports, 2021Microwounding pre-treatment facilitates agroinfiltration and transient gene expression in hard-to-agroinfiltrate citrus varieties. Agrobacterium infiltration is a widely used method for transient expression studies in plants, but this method is not used extensively in citrus because of its low efficiency. In this study, we developed an easy, cheap, and
Stacy Welker +2 more
exaly +4 more sources
Development and optimization of agroinfiltration for soybean
Plant Cell Reports, 2014Agroinfiltration is an efficient method to study transgene expression in plant tissue. In this study, sonication followed by vacuum infiltration is shown to increase agroinfiltration-mediated GUS expression in soybean. Agroinfiltration, a valuable tool for rapid analysis of gene function, has been used extensively on leaf tissue of Nicotiana ...
John Finer, Leah McHale
exaly +3 more sources
Agroinfiltration as a Tool for Transient Expression of cre Recombinase in vivo
Transgenic Research, 2005 Agroinfiltration was used to express transiently cre recombinase from bacteriophage P1 in planta. Activation of gfp expression after cre-mediated excision of a bar intervening sequence served as a marker to monitor site-specific recombination events in lox-target N. benthamiana plants. Gfp expressing regenerants from A.
Lilya, Kopertekh, Joachim, Schiemann
openaire +3 more sources
In Vivo Ubiquitination Assay by Agroinfiltration
Methods in Molecular Biology, 2011The ubiquitination/proteasome system is involved in nearly all plant signaling processes. Many signaling components are degraded by the 26S proteasome upon ubiquitination by specific E3 ubiquitin ligase. However, due to technical limitations, only a few pairs of E3 ligase-substrate interactions have been directly demonstrated in plants.
Qi Xie, Lijing Liu
exaly +3 more sources
Developing a versatile technology for agroinfiltration in multiple plants
bioRxivSummary Agrobacterium -mediated transient transformation methods are widely used in plant science and molecular pharming. However, plant immune response and post-transcriptional gene silencing (PTGS) can compromise gene expression, limiting the adaptability of these methods across plant species.
Tiange Wang +15 more
semanticscholar +2 more sources
Production of bioactive human IFN-γ protein by agroinfiltration in tobacco.
Protein Expression and Purification, 2020The plant-based expression systems are extensively used to produce different biopharmaceuticals such as vaccines, antibodies, growth factors, and cytokines.
Reza Heidari-Japelaghi +4 more
semanticscholar +3 more sources