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Agroinfiltration contributes to VP1 recombinant protein degradation
Bioengineered, 2016 There is a growing interest in applying tobacco agroinfiltration for recombinant protein production in a plant based system. However, in such a system, the action of proteases might compromise recombinant protein production. Protease sensitivity of model recombinant foot-and-mouth disease (FMD) virus P1-polyprotein (P1) and VP1 (viral capsid protein 1)
Priyen Pillay +2 more
exaly +4 more sources
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Development and optimization of agroinfiltration for soybean
Plant Cell Reports, 2014Agroinfiltration is an efficient method to study transgene expression in plant tissue. In this study, sonication followed by vacuum infiltration is shown to increase agroinfiltration-mediated GUS expression in soybean. Agroinfiltration, a valuable tool for rapid analysis of gene function, has been used extensively on leaf tissue of Nicotiana ...
Jessica L King +2 more
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A simple and efficient agroinfiltration method for transient gene expression in Citrus
Plant Cell Reports, 2021Microwounding pre-treatment facilitates agroinfiltration and transient gene expression in hard-to-agroinfiltrate citrus varieties. Agrobacterium infiltration is a widely used method for transient expression studies in plants, but this method is not used extensively in citrus because of its low efficiency. In this study, we developed an easy, cheap, and
Stacy Welker +2 more
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Local RNA Silencing Mediated by Agroinfiltration
Methods in Molecular Biology, 2011Agroinfiltration is a very fast and powerful method to express in planta any sequences in a transient fashion. Agroinfiltration has proven very useful for the overexpression of proteins in the infiltrated zone when a short-term effect can be informative.
Elena Dadami, Kríton Kalantidis
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Transient Expression of Antibodies in Plants Using Syringe Agroinfiltration
Methods in Molecular Biology, 2009The improvements in agroinfiltration methods for plant-based transient expression now allow the production of significant amounts of recombinant proteins in a matter of days. While vacuum-based agroinfiltration has been brought to large scale to meet the cost, speed and surge capacity requirements for vaccine and therapeutic production, the more ...
Pierre-Olivier Lavoie
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Robust soybean leaf agroinfiltration
Plant Cell ReportsA robust agroinfiltration-mediated transient gene expression method for soybean leaves was developed. Plant genotype, developmental stage and leaf age, surfactant, and Agrobacterium culture conditions are important for successful agroinfiltration.
Mst Shamira Sultana +2 more
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Agroinfiltration as a Tool for Transient Expression of cre Recombinase in vivo
Transgenic Research, 2005Agroinfiltration was used to express transiently cre recombinase from bacteriophage P1 in planta. Activation of gfp expression after cre-mediated excision of a bar intervening sequence served as a marker to monitor site-specific recombination events in lox-target N. benthamiana plants. Gfp expressing regenerants from A.
Joachim Schiemann
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Monitoring Pseudomonas syringae Growth in Agroinfiltrated Leaves: The “Agromonas” Assay
2022We recently developed a simple method called "agromonas" which facilitates the detection of Pseudomonas syringae growth in agroinfiltrated leaves expressing genes of interest. This protocol consists of agroinfiltration followed by inoculation of Pseudomonas syringae by either infiltration or spray inoculation.
Pierre Buscaill +1 more
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Delivery and Expression of Functional Viral RNA Genomes In Planta by Agroinfiltration
Current Protocols in Microbiology, 2006AbstractAgroinfiltration is a simple, efficient, and powerful approach for transient expression of viral genes as well as DNA‐based expression of full‐length RNA genomes of plant viruses for studies leading to understanding of replication, movement, and assembly.
A L N Rao
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Production of bioactive human IFN-γ protein by agroinfiltration in tobacco
Protein Expression and Purification, 2020In animals, interferon-γ (IFN-γ) is known as a cytokine involved in antiviral and anticancer activities with a higher biochemical activity in contrast to other IFNs. To produce recombinant human IFN-γ (hIFN-γ) protein in tobacco, factors influencing gene delivery were first evaluated for higher efficiency of transient expression by fluorometric ...
Reza, Heidari-Japelaghi +4 more
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