Results 201 to 210 of about 7,887 (237)

Vacuum-Forced Agroinfiltration for In planta Transformation of Recalcitrant Plants: Cacao as a Case Study.

Journal of Visualized Experiments, 2023
Transient in planta transformation is a fast and cost-effective alternative for plant genetic transformation. Most protocols for in planta transformation rely on the use of Agrobacterium-mediated transformation.
Hilda E. Hernández-Delgado, J. A. Zamora-Briseño, L. Figueroa-Yáñez, Rafael Urrea-López   +3 more
semanticscholar   +1 more source

Evaluating Extraction and Storage of a Recombinant Protein Produced in Agroinfiltrated Lettuce

Biotechnology Progress, 2006
Extraction and storage of a recombinant protein produced by transient expression following agroinfiltration of lettuce were investigated. Lettuce leaves expressing beta-glucuronidase (GUS) were extracted by homogenization in several buffer combinations, and the yield and stability were assessed.
Lawrence D, Joh, Karen A, McDonald, Jean S, VanderGheynst   +2 more
openaire   +2 more sources

Local RNA Silencing Mediated by Agroinfiltration

2011
Agroinfiltration is a very fast and powerful method to express in planta any sequences in a transient fashion. Agroinfiltration has proven very useful for the overexpression of proteins in the infiltrated zone when a short-term effect can be informative.
Jutta Maria, Helm, Elena, Dadami, Kriton, Kalantidis   +2 more
openaire   +2 more sources

Transient Expression Assay by Agroinfiltration of Leaves

2006
An Agrobacterium-mediated transient expression assay has been described for in vivo analysis of constitutive or inducible gene expression in Arabidopsis plants. By simple infiltration of Agrobacterium cells carrying appropriate gene constructs into Arabidopsis leaves, transient expression assays can be performed within 3 d without using expensive ...
Min Woo, Lee, Yinong, Yang
openaire   +2 more sources

Transient Protein Expression by Agroinfiltration in Lettuce

2016
Current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. However, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant ...
Qiang, Chen, Matthew, Dent, Jonathan, Hurtado, Jake, Stahnke, Alyssa, McNulty, Kahlin, Leuzinger, Huafang, Lai   +6 more
openaire   +2 more sources

Delivery and Expression of Functional Viral RNA Genomes In Planta by Agroinfiltration

Current Protocols in Microbiology, 2006
AbstractAgroinfiltration is a simple, efficient, and powerful approach for transient expression of viral genes as well as DNA‐based expression of full‐length RNA genomes of plant viruses for studies leading to understanding of replication, movement, and assembly.
Padmanaban, Annamalai, A L N, Rao
openaire   +2 more sources

Selection of suitable lily cultivars by using needle agroinfiltration for blue flower production

Journal of Horticultural Science & Biotechnology, 2022
Lily is one of the most important flower crops that it lacks blue-purple flowers due to the absence of the flavonoid 3’,5’-hydroxylase gene and deficiency of delphinidin accumulation.
Malihe Fallahpour, A. Ghanbari, P. Koobaz, E. Chamani, P. Azadi, M. Mii   +5 more
semanticscholar   +1 more source

Transient Expression of Chi42 Genes from Trichoderma asperellum in Nicotiana benthamiana by Agroinfiltration

International Journal of Agriculture and Biology, 2021
The present study reports the transient expression of chi42 genes encoding 42 kDa chitinase from T. asperellum SH16 in N. benthamiana via agroinfiltration.
T. Nguyen
semanticscholar   +1 more source

Transient Expression Using Agroinfiltration to Study Polyadenylation in Plants

2014
An important facet of the study of polyadenylation in plants entails the use of transient expression to characterize poly(A) sites and the subunits of the polyadenylation complex. In this report, a simple and adaptable approach to transient expression in Nicotiana benthamiana leaves is described.
Carol, Von Lanken, Arthur G, Hunt
openaire   +2 more sources

Agroinfiltration Transient Assay to Evaluate the Potential of ihpRNA Constructs for Lily Mottle Virus Resistance

International Journal of Agriculture and Biology
In order to obtain a resistant strain of Lily mottle virus (LMoV), three RNA interference (RNAi) vectors with intron hairpin RNA structure (ihpRNA) were constructed and transformed into Agrobacterium GV 3101 strain.
Pinsan Xu, Y. Qi, Xin-Lei Wang, Zheng-Yao Zhang   +3 more
semanticscholar   +1 more source