Results 211 to 220 of about 8,861 (240)
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In Vivo Ubiquitination Assay by Agroinfiltration
2011The ubiquitination/proteasome system is involved in nearly all plant signaling processes. Many signaling components are degraded by the 26S proteasome upon ubiquitination by specific E3 ubiquitin ligase. However, due to technical limitations, only a few pairs of E3 ligase-substrate interactions have been directly demonstrated in plants.
Lijing, Liu, Qingzhen, Zhao, Qi, Xie
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Evaluating Extraction and Storage of a Recombinant Protein Produced in Agroinfiltrated Lettuce
Biotechnology Progress, 2006Extraction and storage of a recombinant protein produced by transient expression following agroinfiltration of lettuce were investigated. Lettuce leaves expressing beta-glucuronidase (GUS) were extracted by homogenization in several buffer combinations, and the yield and stability were assessed.
Lawrence D, Joh +2 more
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Transient Expression Assay by Agroinfiltration of Leaves
2006An Agrobacterium-mediated transient expression assay has been described for in vivo analysis of constitutive or inducible gene expression in Arabidopsis plants. By simple infiltration of Agrobacterium cells carrying appropriate gene constructs into Arabidopsis leaves, transient expression assays can be performed within 3 d without using expensive ...
Min Woo, Lee, Yinong, Yang
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Transient Protein Expression by Agroinfiltration in Lettuce
2016Current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. However, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant ...
Qiang, Chen +6 more
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Transient Expression Using Agroinfiltration to Study Polyadenylation in Plants
2014An important facet of the study of polyadenylation in plants entails the use of transient expression to characterize poly(A) sites and the subunits of the polyadenylation complex. In this report, a simple and adaptable approach to transient expression in Nicotiana benthamiana leaves is described.
Carol, Von Lanken, Arthur G, Hunt
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A simple agroinfiltration method for transient gene expression in plant leaf discs
Journal of Bioscience and Bioengineering, 2016In the present study, we developed a simple transient gene expression system based on Agrobacterium-mediated transformation. Vacuum infiltration was applied to leaf discs from Nicotiana benthamiana plants with Agrobacterium suspension solution under conventional vacuum conditions in a needleless plastic syringe.
Kouki Matsuo +2 more
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Agroinoculation and Agroinfiltration: Simple Tools for Complex Gene Function Analyses
2010Agroinoculation, first developed as a simple tool to study plant-virus interactions, is a popular method of choice for functional gene analysis of viral genomes. With the explosive growth of genomic information and the development of advanced vectors to dissect plant gene function, this reliable method of viral gene delivery in plants, has been ...
Zarir, Vaghchhipawala +3 more
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Transient Gene Expression in Medicago truncatula Leaves via Agroinfiltration
2013Transient expression is a powerful method for the functional characterization of genes. In this chapter, we outline a protocol for the transient expression of constructs in Medicago truncatula leaves using Agrobacterium tumefaciens infiltration. Using quantitative real-time PCR we demonstrate that the infiltration of a construct containing the LEGUME ...
Picard, Kelsey +3 more
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In vivo analysis of plant promoters and transcription factors by agroinfiltration of tobacco leaves
The Plant Journal, 2000SummaryA convenient, Agrobacterium‐mediated transient expression assay has been evaluated for rapid analysis of plant promoters and transcription factors in vivo. By simple infiltration of Agrobacterium cells carrying appropriate plasmid constructs into tobacco leaves in planta, reproducible expression assays could be conducted in as little as 2–3 days
Y, Yang, R, Li, M, Qi
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Purification of His-Tagged Proteases from the Apoplast of Agroinfiltrated N. benthamiana
2022Protein expression in plants by agroinfiltration and subsequent purification is increasingly used for the biochemical characterization of plant proteins. In this chapter we describe the purification of secreted, His-tagged proteases from the apoplast of agroinfiltrated Nicotiana benthamiana using immobilized metal affinity chromatography (IMAC).
Schuster, Mariana +3 more
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