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Transient Expression of Antibodies in Plants Using Syringe Agroinfiltration
2009The improvements in agroinfiltration methods for plant-based transient expression now allow the production of significant amounts of recombinant proteins in a matter of days. While vacuum-based agroinfiltration has been brought to large scale to meet the cost, speed and surge capacity requirements for vaccine and therapeutic production, the more ...
Marc-André, D'Aoust +5 more
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In vivo analysis of plant promoters and transcription factors by agroinfiltration of tobacco leaves
The Plant Journal, 2000SummaryA convenient, Agrobacterium‐mediated transient expression assay has been evaluated for rapid analysis of plant promoters and transcription factors in vivo. By simple infiltration of Agrobacterium cells carrying appropriate plasmid constructs into tobacco leaves in planta, reproducible expression assays could be conducted in as little as 2–3 days
Y, Yang, R, Li, M, Qi
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Agroinoculation and Agroinfiltration: Simple Tools for Complex Gene Function Analyses
2010Agroinoculation, first developed as a simple tool to study plant-virus interactions, is a popular method of choice for functional gene analysis of viral genomes. With the explosive growth of genomic information and the development of advanced vectors to dissect plant gene function, this reliable method of viral gene delivery in plants, has been ...
Zarir, Vaghchhipawala +3 more
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Pesticide Biochemistry and Physiology
The vegetable leafminer (Liriomyza sativae) is a devastating invasive pest of many vegetable crops and horticultural plants worldwide, causing serious economic loss.
Shu-Peng Li +9 more
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The vegetable leafminer (Liriomyza sativae) is a devastating invasive pest of many vegetable crops and horticultural plants worldwide, causing serious economic loss.
Shu-Peng Li +9 more
semanticscholar +1 more source
Purification of His-Tagged Proteases from the Apoplast of Agroinfiltrated N. benthamiana
2022Protein expression in plants by agroinfiltration and subsequent purification is increasingly used for the biochemical characterization of plant proteins. In this chapter we describe the purification of secreted, His-tagged proteases from the apoplast of agroinfiltrated Nicotiana benthamiana using immobilized metal affinity chromatography (IMAC).
Schuster, Mariana +3 more
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Analysis of RNA Silencing in Agroinfiltrated Leaves of Nicotiana Benthamiana and Nicotiana Tabacum
Plant Molecular Biology, 2005In this study we analyse several aspects of cytoplasmic RNA silencing by agroinfiltration of DNA constructs encoding single- and double-stranded RNAs derived from a GFP transgene and from the endogenous Virp1 gene. Both types of inductors resulted after 2-4 days in much higher concentration of siRNAs in the agroinfiltrated zone than normally seen ...
Edyta, Kościańska +4 more
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Studying NB-LRR Immune Receptor Localization by Agroinfiltration Transient Expression
2011NB-LRR immune receptors in plants play dual roles as sentries and as activators of defense. The site in the cell where these activities take place can be different for different NB-LRRs. Furthermore, recognition and defense activation can occur in distinct subcellular compartments. Therefore, determining the subcellular localization of NB-LRRs is a key
Patrick, Cournoyer, S P, Dinesh-Kumar
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[Agroinfiltration, a useful technique in plant molecular biology research].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2002Agroinfiltration is a newly developed plant transient gene expression technique, which is simple, rapid and reproducible. It has been widely used in analyses of foreign gene expression, hypersensitive reaction, gene silencing, promoter activity and identification of new disease-resistance genes.
Zhao-Ming, Liu +3 more
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2013
Protein-protein interactions play important roles in many cellular processes, including the regulation of phytohormone signaling pathways. Identification of interacting partners of key proteins involved in the cellular signaling control can provide potentially unexpected insights into the molecular events occurring in any signaling pathway.
Çevik, Volkan, Kazan, Kemal
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Protein-protein interactions play important roles in many cellular processes, including the regulation of phytohormone signaling pathways. Identification of interacting partners of key proteins involved in the cellular signaling control can provide potentially unexpected insights into the molecular events occurring in any signaling pathway.
Çevik, Volkan, Kazan, Kemal
openaire +4 more sources