Results 221 to 230 of about 8,861 (240)

Analysis of RNA Silencing in Agroinfiltrated Leaves of Nicotiana Benthamiana and Nicotiana Tabacum

Plant Molecular Biology, 2005
In this study we analyse several aspects of cytoplasmic RNA silencing by agroinfiltration of DNA constructs encoding single- and double-stranded RNAs derived from a GFP transgene and from the endogenous Virp1 gene. Both types of inductors resulted after 2-4 days in much higher concentration of siRNAs in the agroinfiltrated zone than normally seen ...
Edyta, Kościańska, Kriton, Kalantidis, Krzysztof, Wypijewski, Jan, Sadowski, Martin, Tabler   +4 more
openaire   +2 more sources

Studying NB-LRR Immune Receptor Localization by Agroinfiltration Transient Expression

2011
NB-LRR immune receptors in plants play dual roles as sentries and as activators of defense. The site in the cell where these activities take place can be different for different NB-LRRs. Furthermore, recognition and defense activation can occur in distinct subcellular compartments. Therefore, determining the subcellular localization of NB-LRRs is a key
Patrick, Cournoyer, S P, Dinesh-Kumar
openaire   +2 more sources

Transient gene expression in western white pine using agroinfiltration

Journal of Forestry Research, 2019
A method for transient gene expression was developed for western white pine (WWP, Pinus monticola Dougl. ex D.Don) using reporter gene uidA encoding β-glucuronidase (GUS). GUS was transiently expressed in cross sections of primary and secondary needles, cotyledons, and current and second year stems of WWP via vacuum-infiltration with Agrobacterium ...
Zhenguo Ma, Jun-Jun Liu, Arezoo Zamany, Holly Williams   +3 more
openaire   +1 more source

Direct creation of marker-free tobacco plants from agroinfiltrated leaf discs

Plant Cell Reports, 2007
Agroinfiltration is employed as a fast way to directly create marker-free transgenic tobacco plants. As an example for the efficiency of the method, Agrobacterium cells harboring a marker-free vector coding for beta-glucuronidase (GUS) were infiltrated into the leaf discs of Nicotiana tabacum, which were then used as explants for marker-free plant ...
Jia, Jongge, Liao, Mingjun, Verbelen, Jean-Pierre, Vissenberg, Kris   +3 more
openaire   +3 more sources

[Agroinfiltration, a useful technique in plant molecular biology research].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2002
Agroinfiltration is a newly developed plant transient gene expression technique, which is simple, rapid and reproducible. It has been widely used in analyses of foreign gene expression, hypersensitive reaction, gene silencing, promoter activity and identification of new disease-resistance genes.
Zhao-Ming, Liu, Zong-Zhi, Liu, Qing-Wu, Bai, Rong-Xiang, Fang   +3 more
openaire   +1 more source

Developing a versatile technology for agroinfiltration in multiple plants

Summary Agrobacterium -mediated transient transformation methods are widely used in plant science and molecular pharming. However, plant immune response and post-transcriptional gene silencing (PTGS) can compromise gene expression, limiting the adaptability of these methods across plant species.
Tiange Wang, Jieyu Ge, Chengyi Qu, Hongyu Chen, Jie Li, Shun Wang, Xueying Guan, Tao Chen, Zhanfeng Si, Keming Hu, Xinhua Ding, Li Liu, Jian Zhang, Alexander S. Mishin, Ilia V. Yampolsky, Hao Du   +15 more
openaire   +1 more source

Vacuum-Based Agroinfiltration for Transient Gene Expression in Arabidopsis Seedlings

Agrobacterium-mediated transient expression is a flexible and efficient technique for introducing genes into plants, allowing for rapid and temporary gene expression. Agroinfiltration of Arabidopsis seedlings is a newly developed Agrobacterium-based transient expression system.
Linjuan, Shi, Dongmei, Zhu, Wenze, Zhang, Jinbo, Shen, Shuai, Hu   +4 more
openaire   +2 more sources

Agroinfiltration for Enhanced Transgene Expression in Coffee Leaves (Coffea arabica L.)

The Coffea spp. plant is a significant crop in Latin America, Africa, and Asia, and recent advances in genomics and transcriptomics have opened possibilities for studying candidate genes and introducing new desirable traits through genetic engineering. While stable transformation of coffee plants has been reported using various techniques, it is a time-
Andrés, Gatica-Arias, Luiz Filipe, Pereira   +1 more
openaire   +2 more sources

Transient Expression Using Agroinfiltration and Its Applications in Molecular Farming

2011
Transient expression via agroinfiltration and/or viral vectors has quickly emerged as the preferred expression system for plant-made recombinant proteins. Transient expression can serve as a valuable research tool for finding optimal expression parameters before tedious and time-consuming production of stable transgenic plants or it can be scaled up to
Menassa, Rima, Ahmad, Adil, Joensuu, Jussi   +2 more
openaire   +1 more source

Transient Expression of CCL21Chemokine in Tobacco via Agroinfiltration

International Journal of Scientific & Engineering Research, 2016
Maria Beihaghi, Hasan Marashi, Abdolreza Bagheri, Mojtaba Sankian   +3 more
openaire   +1 more source