Results 81 to 90 of about 688,717 (308)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

AI-2 of Aggregatibacter actinomycetemcomitans inhibits Candida albicans biofilm formation [PDF]

Frontiers in Cellular and Infection Microbiology, 2014
Aggregatibacter actinomycetemcomitans, a Gram-negative bacterium, and Candida albicans, a polymorphic fungus, are both commensals of the oral cavity but both are opportunistic pathogens that can cause oral diseases. A. actinomycetemcomitans produces a quorum-sensing molecule called autoinducer-2 (AI-2), synthesized by LuxS, that plays an important role
Bachtiar, E.W., Bachtiar, B.M., Jarosz, L.M., Amir, L.R., Sunarto, H., Ganin, H., Meijler, M.M., Krom, B.P.   +7 more
openaire   +4 more sources

Linearized Alternating Direction Method with Parallel Splitting and Adaptive Penalty for Separable Convex Programs in Machine Learning

, 2014
Many problems in machine learning and other fields can be (re)for-mulated as linearly constrained separable convex programs. In most of the cases, there are multiple blocks of variables. However, the traditional alternating direction method (ADM) and its
Li, Huan, Lin, Zhouchen, Liu, Risheng
core   +1 more source

SIRT4 positively regulates autophagy via ULK1, but independently of HDAC6 and OPA1

FEBS Open Bio, EarlyView.
Cells expressing SIRT4 (H161Y), a catalytically inactive mutant of the sirtuin SIRT4, fail to upregulate LC3B‐II and exhibit a reduced autophagic flux under stress conditions. Interestingly, SIRT4(H161Y) promotes phosphorylation of ULK1 at S638 and S758 that are associated with inhibition of autophagy initiation.
Isabell Lehmkuhl, Khawar Amin, Lydia Gabriel, Nils Hampel, Afshin Iram, Julia Hesse, Constanze Wiek, Jasmin Thuy Vy Nguyen, Helmut Hanenberg, Jürgen Scheller, M. Reza Ahmadian, Björn Stork, Doreen M. Floss, Roland P. Piekorz   +13 more
wiley   +1 more source

Gauge invariant perturbation theory and non-critical string models of Yang-Mills theories

, 2010
We carry out a gauge invariant analysis of certain perturbations of $D-2$-branes solutions of low energy string theories. We get generically a system of second order coupled differential equations, and show that only in very particular cases it is ...
A Fotopoulos, A Hashimoto, Adrián R. Lugo, AM Polyakov, AM Polyakov, AR Lugo, AR Lugo, C Csáki, CJ Morningstar, D Kutasov, DJ Gross, E Caceres, E Kiritsis, E Witten, E Witten, IR Klebanov, IR Klebanov, J Polchinski, JA Minahan, JM Bardeen, JM Maldacena, JM Maldacena, JM Maldacena, K Nakamura, Mauricio B. Sturla, MJ Teper, N Itzhaki, NR Constable, O Aharony, R Mello Koch de, RC Brower, RC Brower, S Kuperstein, S Kuperstein, S Murthy, S-J Rey, SK Ashok, SS Gubser, U Gürsoy, Y Kinar   +39 more
core   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Numerical model for jointed rock mass in particle flow code and its application

Yantu gongcheng xuebao, 2017
To simulate the joint network around mining tunnels and to conduct the stability analysis of tunnels in jointed rock mass, a numerical model for jointed rock mass containing particle flow block model, original joint network and secondary joint network is
JIN Ai-bing 1, LIU Gang 1, YANG Zhen-wei 2
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Incorporating LsrK AI‐2 quorum quenching capability in a functionalized biopolymer capsule

Biotechnology and Bioengineering, 2017
AbstractAntibacterial resistance is an issue of increasing severity as current antibiotics are losing their effectiveness and fewer antibiotics are being developed. New methods for combating bacterial virulence are required. Modulating molecular communication among bacteria can alter phenotype, including attachment to epithelia, biofilm formation, and ...
Rhoads, Melissa K., Hauk, Pricila, Terrell, Jessica Lynn, Tsao, Chen-Yu, Oh, Hyuntaek, Raghavan, Srinivasa R., Mansy, Sheref S., Payne, Gregory F., Bentley, William E.   +8 more
openaire   +3 more sources

The number of cubic partitions modulo powers of 5

, 2010
The notion of cubic partitions is introduced by Hei-Chi Chan and named by Byungchan Kim in connection with Ramanujan's cubic continued fractions. Chan proved that cubic partition function has Ramanujan Type congruences modulo powers of $3$.
Xiong, Xinhua
core   +1 more source