Distribution and phylogenies of enzymes of the Embden-Meyerhof-Parnas pathway from archaea and hyperthermophilic bacteria support a gluconeogenic origin of metabolism [PDF]
Enzymes of the gluconeogenic/glycolytic pathway (the Embden‐Meyerhof‐Parnas (EMP) pathway), the reductive tricarboxylic acid cycle, the reductive pentose phosphate cycle and the Entner‐Doudoroff pathway are widely distributed and are often considered to be central to the origins of metabolism.
Hugh W. Morgan, Ron S. Ronimus
core +2 more sources
Biochemical and Structural Characterisation of a Novel D-Lyxose Isomerase From the Hyperthermophilic Archaeon Thermofilum sp. [PDF]
This is the final version. Available on open access from Frontiers Media via the DOI in this recordData Availability Statement: The atomic coordinates and structure factors for the crystal structures of TsLI and its complexes have been deposited in the ...
De Rose SA +5 more
europepmc +5 more sources
Enzymatic Upgrading of Biomass-Derived Aldoses to Rare Deoxy Ketoses Catalyzed by Transketolase Variants. [PDF]
A sustainable, convenient, scalable, one‐step method for the two‐carbon chain elongation of cheap, biomass‐derived pentoses (l‐arabinose, and 2‐deoxy‐d‐ribose) and a hexose (l‐rhamnose) was developed to produce Cn+2 deoxy ketoses (C‐7 and C‐8) using transketolase, an enzyme catalyzing the irreversible transfer of a ketol group from an α‐ketoacid to an ...
Arbia G +6 more
europepmc +2 more sources
Substrate Induced Movement of the Metal Cofactor between Active and Resting State
Pyruvate dependent Class II aldolases require a ketoacid to induce a metal shift from resting state to active enzyme. This occurs without alterations in the protein structure and might be a common, but to date overlooked, feature in many metalloproteins. Abstract Regulation of enzyme activity is vital for living organisms.
Stefan R. Marsden +11 more
wiley +2 more sources
Aroma and color development during the production of specialty malts: A review
Abstract Specialty malts comprise a promising field for innovative approaches concerning their potential in terms of color, aroma, and taste influence on the composition of beer and other beverages. Nevertheless, poor reproducibility of aroma and taste is a recurrent struggle between maltsters, leaving color as a practical parameter for quality control.
Raphael Prado +2 more
wiley +1 more source
Thermotolerance and Hydrogen Production Potential of Salt-tolerant Microflora from Salt Fields [PDF]
Upper mesophilic temperature acclimation of halophilic, hydrogen-producing bacteria from salt fields was investigated in this study, along with the changes in microbial abundance during anaerobic digestion (AD) process.
Chairattanamanokorn, Prapaipid +5 more
core +2 more sources
The anoxic electrode‐driven fructose catabolism of Pseudomonas putida KT2440
A new pathway was uncovered for the fructose metabolism in Pseudomonas putida KT2440 cultivated anaerobically in a bioelectrochemical system. In addition to the typical PTS transporter‐based pathway observed under aerobic condition, fructose was firstly converted to mannose by isomerization and then oxidized to mannonate by the glucose dehydrogenase ...
Anh Vu Nguyen +3 more
wiley +1 more source
Xylose isomerase from Piromyces:Characterization and engineering for improving S. cerevisiae-based lignocellulosic bioethanol production [PDF]
Lignocellulosic bioethanol has been recognized as a possible fossil fuel alternative. This so-called “second-generation” bioethanol has gained growing interest as it can be produced using agricultural waste material or side products in the food industry ...
Lee, Misun
core +1 more source
Crystal structure of a novel homodimeric l‐ribulose 3‐epimerase from Methylomonus sp.
d‐Allulose has potential as a low‐calorie sweetener which can suppress fat accumulation. The enzymes capable of d‐allulose production have attracted attention. Here, we report the X‐ray structure of a novel l‐ribulose 3‐epimerase from Methylomonas sp.
Hiromi Yoshida +6 more
wiley +1 more source
Structure-based directed evolution improves S. cerevisiae growth on xylose by influencing in vivo enzyme performance [PDF]
Background: Efficient bioethanol production from hemicellulose feedstocks by Saccharomyces cerevisiae requires xylose utilization. Whereas S. cerevisiae does not metabolize xylose, engineered strains that express xylose isomerase can metabolize xylose by
de Waal, Paul +4 more
core +1 more source

