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Marine Bacteria, A Source for Alginolytic Enzyme to Disrupt Pseudomonas aeruginosa Biofilms
Pseudomonas aeruginosa biofilms are typically associated with the chronic lung infection of cystic fibrosis (CF) patients and represent a major challenge for treatment. This opportunistic bacterial pathogen secretes alginate, a polysaccharide that is one
Said M. Daboor +4 more
doaj +1 more source
Continuous Degradation of Sodium Alginate in Bioreactor Using Immobilized Alginate Lyase.
The enzymatic degradation of sodium alginate was continuously carried out to effectively produce alginate oligosaccharides using immobilized alginate lyase in a CSTR (continuous stirred tank reactor) system. The alginate lyase was immobilized onto the chitosan beads and the reaction was operated at an initial alginate concentration of 10 g l−1 at 35°C ...
MATSUBARA, Yasuhito +2 more
openaire +2 more sources
Catalytically important amino-acid residues of abalone alginate lyase HdAly assessed by site-directed mutagenesis [PDF]
Alginate lyase is an enzyme that degrades alginate chains via β-elimination and has been used for the production of alginate oligosaccharides and protoplasts from brown algae.
Sahara, Takehiko +6 more
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Characterization of a Novel Alginate Lyase from Marine Bacterium Vibrio furnissii H1
Alginate lyases show great potential for industrial and medicinal applications, especially as an attractive biocatalyst for the production of oligosaccharides with special bioactivities.
Xiaoyan Zhu +7 more
doaj +1 more source
Characterization of alginate lyase from Pseudomonas syringae pv. Syringae
The gene encoding alginate lyase (algL) in Pseudomonas syringae pv. syringae was cloned, sequenced, and overexpressed in Escherichia coli. Alginate lyase activity was optimal when the pH was 7.0 and when assays were conducted at 42°C in the presence of 0.
Schiller, Neal L. +3 more
core +1 more source
In order to investigate the catalytic properties of alginate lyase from Pseudomonas aeruginosa CF1/M1, a clinical isolate, regarding the capability to perform β-elimination on oligomannuronates of defined length (2–9), the alginate lyase was purified ...
Rehm, BHA, Rehm, Bernd H.A.
core +1 more source
Marine bacterial alginate lyases play a role in marine alginate degradation and carbon cycling. Although a large number of alginate lyases have been characterized, reports on alginate lyases with special characteristics are still rather less.
Xiu-Lan Chen +19 more
doaj +1 more source
Algin is the most abundant substance in alga. Alginate lyase degrades algin and produces algin monosaccharides, disaccharides, and oligosaccharides, which are widely used in bioenergy, food, medicine, and other fields.
Hanwen Wu +6 more
doaj +1 more source
4-Deoxy-l- erythro -5-hexoseulose uronic acid (DEH) is a rare deoxy sugar produced from alginate by the action of an exotype alginate lyase. A simple and rapid method for analyzing DEH using high-performance liquid chromatography with evaporative light ...
Toshiyuki Shibata +5 more
doaj +1 more source
Seaweed biomass is as an abundant and renewable source of complex polysaccharides, including alginate which has a variety of applications. A sustainable method for exploiting alginate towards the production of valuable oligosaccharides is through ...
Tsopanakis, Vasileios +4 more
core +1 more source

