Results 121 to 130 of about 3,190 (162)

Characteristics and applications of alginate lyases: A review

International Journal of Biological Macromolecules, 2020
Brown algae, as the main source of alginate, are a type of marine biomass with a very high output. Alginate, a polysaccharide composed of β-D-mannuronic acid (M) and α-L-guluronic acid (G), has great potential for applications in the food, cosmetic and pharmaceutical industries. Alginate lyases (Alys) can degrade alginate polymers into oligosaccharides
Danyang, Cheng, Chengcheng, Jiang, Jiachao, Xu, Zhen, Liu, Xiangzhao, Mao   +4 more
openaire   +2 more sources

Bacterial alginate lyase highly active on acetylated alginates

Journal of Fermentation and Bioengineering, 1993
Abstract A bacterium (strain A1) isolated from a ditch synthesized three kinds of intracellular alginate lyases: A1-I (molecular weight [M.W.] 60,000), A1-II-1 (M.W. 60,000) and A1-II-2 (M.W. 25,000) in laboratory-scale cultures. However, when cells of strain A1 were grown on an industrial scale, another lyase (A1-III) was produced other than A1-I ...
Tomohiro Hisano, Minoru Nishimura, Yoshimasa Yonemoto, Shiro Abe, Tetsuo Yamashita, Kenji Sakaguchi, Akira Kimura, Kousaku Murata   +7 more
  +4 more sources

Bacterial alginate lyase: Characterization of alginate lyase-producing bacteria and purification of the enzyme

Journal of Fermentation and Bioengineering, 1991
Abstract Two kinds of bacteria producing alginate lyase were isolated from ditch sample and they were designated A-1 and A-2 strains. Morphological and physiological properties of the two strains were closely similar to each other, except that the cells of strain A-1 showed flocculation during growth on a medium containing alginate as a carbon source.
Yoshimasa Yonemoto, Kousaku Murata, Akira Kimura, Hisako Yamaguchi, Kenichi Okayama   +4 more
openaire   +1 more source

The Surface Display of the Alginate Lyase on the Cells of Yarrowia lipolytica for Hydrolysis of Alginate

Marine Biotechnology, 2009
The alginate lyase structural gene (AlyVI gene) was amplified from plasmid pET24-ALYVI carrying the alginate lyase gene from the marine bacterium Vibrio sp. QY101 which is a pathogen of Laminaria sp. When the gene was cloned into the multiple cloning site of the surface display vector pINA1317-YlCWP110 and expressed in cells of Yarrowia lipolytica, the
Liu, Guanglei, Yue, Lixi, Chi, Zhe, Yu, Wengong, Chi, Zhenming, Madzak, Catherine   +5 more
openaire   +3 more sources

Elucidation of degradation pattern and immobilization of a novel alginate lyase for preparation of alginate oligosaccharides

International Journal of Biological Macromolecules, 2020
Alginate lyases are important enzymes to prepare alginate oligosaccharides for industrial applications and elucidating the degradation pattern of alginate lyases is essential for expanding their applications. Herein, we cloned and expressed AlyPL6, a novel member of polysaccharide lyase family-6 (PL6) with high activity from Pedobacter hainanensis NJ ...
Benwei Zhu, Zhong Yao, Fang Ni
exaly   +3 more sources

Kinetics and specificity of alginate lyases

Hydrobiologia, 1993
A purified preparation of the extracellular alginate lyase has been used to study kinetics and specificity towards purified, homopolymeric fragments of alginate. The enzyme preparation from Bacillus circulans 1351 degraded both block types, although with different efficiency, and thus appears to be nonspecific.
Bj�rn Larsen, Kirsti Ho�en, Kjetill �stgaard   +2 more
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Characterization of Alginate Lyase Activity on Liquid, Gelled, and Complexed States of Alginate

Biotechnology Progress, 2007
A study of alginate lyase was carried out to determine if this enzyme could be used to remove alginate present in the core of alginate/poly-L-lysine (AG/PLL) microcapsules in order to maximize cell growth and colonization. A complete kinetic study was undertaken, which indicated an optimal activity of the enzyme at pH 7-8, 50 degrees C, in the presence
Breguet V, von Stockar U, Marison IW
openaire   +3 more sources

Alginate production by an Azotobacter vinelandii mutant unable to produce alginate lyase

Applied Microbiology and Biotechnology, 2003
Alginate is an industrially relevant linear copolymer composed of beta-1,4-linked D-mannuronic acid and its C-5 epimer L-guluronic acid. The rheological and gel-forming properties of alginates depend on the molecular weight and the relative content of the two monomers. Alginate produced by Azotobacter vinelandii was shown to be degraded towards the end
M A, Trujillo-Roldán, S, Moreno, D, Segura, E, Galindo, G, Espín   +4 more
openaire   +2 more sources

Overexpression of alginate lyase of Pseudoalteromonas elyakovii in Escherichia coli, purification, and characterization of the recombinant alginate lyase

World Journal of Microbiology and Biotechnology, 2007
The alyPEEC gene encoding alginate lyase from marine bacterium Pseudoalteromonas elyakovii IAM 14594 was subcloned into pBAD24 with arabinose promoter and sequenced, and overexpressed in TOP10 strain of E. coli after arabinose induction. Expression levels of alyPEEC gene in E. coli cells were over 39.6-fold higher than those in P.
L. Y. Ma, Z. M. Chi, J. Li, L. F. Wu
openaire   +1 more source

Bacterial alginate lyase inactive on alginate biosynthesized by Pseudomonas aeruginosa

Journal of Fermentation and Bioengineering, 1993
A bacterium (strain Al) isolated from a ditch produces three kinds of intracellular alginate lyases [Al-I (molecular weight: M.W. 60,000), Al-II-1 (M.W. 60,000) and Al-II-2]; the former two lyases have been purified and characterized (Yonemoto et al., J. Ferment. Bioeng., 72, 152–157, 1991).
Tomohiro Hisano, Hisako Yamaguchi, Yoshimasa Yonemoto, Kenji Sakaguchi, Tetsuo Yamashita, Shiro Abe, Akira Kimura, Kousaku Murata   +7 more
openaire   +1 more source