Results 181 to 190 of about 7,283 (209)

Enzymatic Properties of Alginate Lyase from Paenibacillus sp. S29

Applied Biochemistry and Biotechnology, 2017
Paenibacillus sp. S29 was isolated from soil and produces an alginate lyase. The molecular weight of this enzyme was 32 kDa and the N-terminal amino acid sequence was ASVTKST. The optimal pH was approximately 8.7 and the enzyme was stable over a pH range of 5.6 to 8.8 at 40 °C for 60 min.
Masahiro, Kurakake, Yuhei, Kitagawa, Atsushi, Okazaki, Kazuyuki, Shimizu   +3 more
openaire   +2 more sources

Molecular identification of Sphingomonas sp. A1 Alginate lyase (A1-IV′) as a member of novel polysaccharide lyase family 15 and implications in alginate lyase evolution

Journal of Bioscience and Bioengineering, 2005
Sphingomonas sp. A1 (strain A1) produces three endotypes (A1-I [65 kDa], A1-II [25 kDa], and A1-III [40 kDa]) and an exotype (A1-IV [86 kDa]) alginate lyases in cytoplasm. These four enzymes cooperatively depolymerize alginate into constituent monosaccharides.
Wataru, Hashimoto, Osamu, Miyake, Akihito, Ochiai, Kousaku, Murata   +3 more
openaire   +2 more sources

[Cloning and expression of alginate lyase genes from Vibrio alginolyticus and characterization of the alginate lyase].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2019
With the discovery of the significant medicinal value of alginate oligosaccharides and bioethanol produced by microalgae, alginate lyase has been the focus of research in all fields. Five alginate lyase genes in cluster from Vibrio alginolyticus were cloned and expressed in Escherichia coli.
Jie, Gao, Yimin, Li, Cong, Du, Xuze, Pei, Huimin, Lu, Xiaoyang, Zhao, Wenjie, Yuan   +6 more
openaire   +1 more source

Isolation of an alginate-degrading organism and purification of its alginate lyase

Journal of Fermentation and Bioengineering, 1991
Abstract We isolated a bacterial strain, OS-ALG-9, which solubilized immobilized gel with calcium alginate in a reactor. It was identified as Pseudomonas sp. We studied the cultural conditions by using a 2.5- l jar fermentor and found that the growth and alginate-degrading enzyme production were optimal at pH 7.0 and 30°C.
Shinichi Kinoshita, Yasushi Kumoi, Atsumi Ohshima, Toshiomi Yoshida, Naoya Kasai   +4 more
openaire   +1 more source

An exotype alginate lyase in Sphingomonas sp. A1: overexpression in Escherichia coli, purification, and characterization of alginate lyase IV (A1-IV)

Protein Expression and Purification, 2003
Sphingomonas sp. A1 (strain A1) cells contain three kinds of endotype alginate lyases [A1-I, A1-II, and A1-III], all of which are formed from a common precursor through posttranslational processing. In addition to these lyases, another type of lyase (A1-IV) that acts on oligoalginates exists in the bacterium. A1-IV was overexpressed in Escherichia coli
Osamu, Miyake, Wataru, Hashimoto, Kousaku, Murata   +2 more
openaire   +2 more sources

Alginate Lyases of Pseudomonads*

The Journal of Biochemistry, 1969
Y, Kashiwabara, H, Suzuki, K, Nisizawa
openaire   +2 more sources

[Advances in algae tool enzymes: alginate lyases].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2013
Marine can be considered as a rather unexplored source of biological material. Production of algal oligosaccharides by using valuable enzymes from marine origin has become an important way to utilize marine resources. As one of algal tool enzymes, the use of alginate lyases has been focused mainly on development and application of alginate ...
Liyan, Li, Huashi, Guan, Xiaolu, Jiang, Jianjun, Hao   +3 more
openaire   +1 more source

Alginate lyase

1990
Dietmar Schomburg, Margit Salzmann
openaire   +1 more source

Characterization of a New Biofunctional, Exolytic Alginate Lyase from Tamlana sp. s12 with High Catalytic Activity and Cold-Adapted Features

Marine Drugs, 2021
Yan-Xia Zhou
exaly  

Hydrolyzing Laminaria japonica with a combination of microbial alginate lyase and cellulase

Bioresource Technology, 2020
Chixiang Sun, Xiaobin Yu
exaly