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[110] Bacterial alginate lyase
1966Publisher Summary This chapter discusses the synthesis of bacterial alginate lyase. The enzyme occurs in an organism isolated from Potomac Bay mud capable of utilizing alginic acid as a sole carbon source. The organism was classified as a pseudomonad 1 and can be obtained from the American Type Culture Collection.
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Isolation and characterization of an alginate lyase from Klebsiella aerogenes
Archives of Microbiology, 1989The bacterium Klebsiella aerogenes (type 25) produced an inducible alginate lyase, whose major activity was located intracellularly during all growth phases. The enzyme was purified from the soluble fraction of sonicated cells by ammonium sulfate precipitation, anion- and cation-exchange chromatography and gel filtration.
B, Lange, J, Wingender, U K, Winkler
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Food Chemistry
To achieve the cost-effective alginate oligosaccharides production, we have developed a novel rational design strategy that optimized capture behavior, orientation movement and hydrogen bond interaction of substrate in alginate lyase ALYI1. This approach led to S56D and G258Q advantageous variants balancing the trade-off challenge, particularly S56D ...
Jin-Ping, Huang +9 more
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To achieve the cost-effective alginate oligosaccharides production, we have developed a novel rational design strategy that optimized capture behavior, orientation movement and hydrogen bond interaction of substrate in alginate lyase ALYI1. This approach led to S56D and G258Q advantageous variants balancing the trade-off challenge, particularly S56D ...
Jin-Ping, Huang +9 more
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Alginate Lyases of Pseudomonads*
The Journal of Biochemistry, 1969Y, Kashiwabara, H, Suzuki, K, Nisizawa
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International Journal of Biological Macromolecules
Alginate is mainly a linear polysaccharide composed of randomly arranged β-D-mannuronic acid and α-L-guluronic acid linked by α, β-(1,4)-glycosidic bonds. Alginate lyases degrade alginate mainly adopting a β-elimination mechanism, breaking the glycosidic bonds between the monomers and forming a double bond between the C4 and C5 sugar rings to produce ...
Chen, Chen +7 more
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Alginate is mainly a linear polysaccharide composed of randomly arranged β-D-mannuronic acid and α-L-guluronic acid linked by α, β-(1,4)-glycosidic bonds. Alginate lyases degrade alginate mainly adopting a β-elimination mechanism, breaking the glycosidic bonds between the monomers and forming a double bond between the C4 and C5 sugar rings to produce ...
Chen, Chen +7 more
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Characterization of a new endo-type alginate lyase from Vibrio sp. NJU-03
International Journal of Biological Macromolecules, 2018Benwei Zhu, Yun Sun, Fang Ni
exaly