Results 171 to 180 of about 7,283 (209)

Characteristics and applications of alginate lyases: A review

International Journal of Biological Macromolecules, 2020
Brown algae, as the main source of alginate, are a type of marine biomass with a very high output. Alginate, a polysaccharide composed of β-D-mannuronic acid (M) and α-L-guluronic acid (G), has great potential for applications in the food, cosmetic and pharmaceutical industries. Alginate lyases (Alys) can degrade alginate polymers into oligosaccharides
Danyang, Cheng, Chengcheng, Jiang, Jiachao, Xu, Zhen, Liu, Xiangzhao, Mao   +4 more
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Bacterial alginate lyase inactive on alginate biosynthesized by Pseudomonas aeruginosa

Journal of Fermentation and Bioengineering, 1993
A bacterium (strain Al) isolated from a ditch produces three kinds of intracellular alginate lyases [Al-I (molecular weight: M.W. 60,000), Al-II-1 (M.W. 60,000) and Al-II-2]; the former two lyases have been purified and characterized (Yonemoto et al., J. Ferment. Bioeng., 72, 152–157, 1991).
Tomohiro Hisano, Hisako Yamaguchi, Yoshimasa Yonemoto, Kenji Sakaguchi, Tetsuo Yamashita, Shiro Abe, Akira Kimura, Kousaku Murata   +7 more
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Alginate lyase: Structure, property, and application

Biotechnology and Bioprocess Engineering, 2011
Alginate is a linear polysaccharide in which β-D-mannuronate (M) and its epimer, α-L-guluronate (G), are covalently (1–4)-linked in different sequences. Alginate is mainly used as a food additive to modify food texture due to its high viscosity and gelling property.
Hee Sook Kim, Choul-Gyun Lee, Eun Yeol Lee   +2 more
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Kinetics and specificity of alginate lyases

Hydrobiologia, 1993
A purified preparation of the extracellular alginate lyase has been used to study kinetics and specificity towards purified, homopolymeric fragments of alginate. The enzyme preparation from Bacillus circulans 1351 degraded both block types, although with different efficiency, and thus appears to be nonspecific.
Bj�rn Larsen, Kirsti Ho�en, Kjetill �stgaard   +2 more
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Alginate production by an Azotobacter vinelandii mutant unable to produce alginate lyase

Applied Microbiology and Biotechnology, 2003
Alginate is an industrially relevant linear copolymer composed of beta-1,4-linked D-mannuronic acid and its C-5 epimer L-guluronic acid. The rheological and gel-forming properties of alginates depend on the molecular weight and the relative content of the two monomers. Alginate produced by Azotobacter vinelandii was shown to be degraded towards the end
M A, Trujillo-Roldán, S, Moreno, D, Segura, E, Galindo, G, Espín   +4 more
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Preparation of Dye-labeled Alginate for the Assay of Alginate Lyase

Bioscience, Biotechnology, and Biochemistry, 1997
Dabsyl-alginate, the dye-labeled substrate of alginate lyase, was prepared. Low-viscosity alginate, which was prepared by enzymatic degradation of sodium alginate, and tetramethylenediamine were conjugated by reductive amination. Then, dabsyl-Cl was coupled with the primary amino group of the aminobutyl-alginate. The assay for endo-alginate lyase using
Shigeki Yoshida, Sayaka Watanabe, Toshio Takeuchi, Katsumi Murata, Isao Kusakabe   +4 more
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Characterization of Alginate Lyase Activity on Liquid, Gelled, and Complexed States of Alginate

Biotechnology Progress, 2007
A study of alginate lyase was carried out to determine if this enzyme could be used to remove alginate present in the core of alginate/poly-L-lysine (AG/PLL) microcapsules in order to maximize cell growth and colonization. A complete kinetic study was undertaken, which indicated an optimal activity of the enzyme at pH 7-8, 50 degrees C, in the presence
Véronique, Breguet, Urs, von Stockar, Ian W, Marison   +2 more
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Degradable alginate hydrogel microfiber for cell-encapsulation based on alginate lyase loaded nanoparticles

Materials Today Communications, 2021
Abstract Cell-encapsulation in hydrogels is a promising strategy for tissue engineering and cell therapy, particularly alginate hydrogels as they immobilize the cells in porous matrices, which allows an exchange of nutrients and oxygen and protects the cells from immune clearance.
Wisawat Keaswejjareansuk, Somrudee Keawmaloon, Nuttawat Sawangrat, Satit Puttipipatkhachorn, Teerapong Yata, Phornphimon Maitarad, Liyi Shi, Mattaka Khongkow, Katawut Namdee   +8 more
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[110] Bacterial alginate lyase

1966
Publisher Summary This chapter discusses the synthesis of bacterial alginate lyase. The enzyme occurs in an organism isolated from Potomac Bay mud capable of utilizing alginic acid as a sole carbon source. The organism was classified as a pseudomonad 1 and can be obtained from the American Type Culture Collection.
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Overexpression of alginate lyase of Pseudoalteromonas elyakovii in Escherichia coli, purification, and characterization of the recombinant alginate lyase

World Journal of Microbiology and Biotechnology, 2007
The alyPEEC gene encoding alginate lyase from marine bacterium Pseudoalteromonas elyakovii IAM 14594 was subcloned into pBAD24 with arabinose promoter and sequenced, and overexpressed in TOP10 strain of E. coli after arabinose induction. Expression levels of alyPEEC gene in E. coli cells were over 39.6-fold higher than those in P.
L. Y. Ma, Z. M. Chi, J. Li, L. F. Wu
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