Overproduction and properties of the mannuronate alginate lyase AlxMB [PDF]
In previous studies (Malissard et al., FEMS Microbiol. Lett. (1993) 110, 101-106), the alginate lyase AlxM of the marine bacterium ATCC 433367 was produced in Escherichia coli TC4/pAL-A3 with a yield of 50 micrograms per litre of culture. The polypeptide chain was cleaved between two cysteine residues, C169 and C183, themselves linked by a disulphide ...
Malissard, Martine +6 more
openaire +4 more sources
Two types of bacterial alginate lyases [PDF]
The extracellular alginate lyases were purified from Vibrio harveyi AL-128 and V. alginolyticus ATCC 17749. The former enzyme appears to be specific for alpha-1,4 bonds involving L-guluronate units in alginate, whereas the latter exhibits specificity for beta-1,4 bonds involving D-mannuronate units.
Manabu Kitamikado +3 more
+6 more sources
Characterization of a Novel Alginate Lyase from Marine Bacterium Vibrio furnissii H1 [PDF]
Alginate lyases show great potential for industrial and medicinal applications, especially as an attractive biocatalyst for the production of oligosaccharides with special bioactivities.
Xiaoyan Zhu +7 more
doaj +2 more sources
Insights into the mechanism of substrate specificity in a novel PL15_3 subfamily oligo-alginate lyase VBAly15A. [PDF]
Tang Y, Song Z, Xu X, Li Y, Wang L.
europepmc +2 more sources
An individual alginate lyase is effective in the disruption of Laminaria digitata recalcitrant cell wall [PDF]
In the present study, 199 pre-selected Carbohydrate-Active enZymes (CAZymes) and sulfatases were assessed, either alone or in combination, to evaluate their capacity to disrupt Laminaria digitata cell wall, with the consequent release of interesting ...
Mónica Costa +8 more
doaj +2 more sources
Exploring the Catalytic Mechanisms of a Newly Identified Salt-Activated Alginate Lyase from <i>Pseudoalteromonas carrageenovora</i> ASY5. [PDF]
Zhuang X +11 more
europepmc +3 more sources
Improving the thermostability of alginate lyase FlAlyA with high expression by computer-aided rational design for industrial preparation of alginate oligosaccharides. [PDF]
Zhang X +8 more
europepmc +3 more sources
Synergy of the Two Alginate Lyase Domains of a Novel Alginate Lyase from Vibrio sp. NC2 in Alginate Degradation. [PDF]
Alginate is a major component in the cell walls of brown algae. Alginate degradation is carried out by alginate lyases.
Wang XH +6 more
europepmc +3 more sources
Biochemical Characterization of a Novel Exo-Type PL7 Alginate Lyase VsAly7D from Marine Vibrio sp. QY108. [PDF]
Zhang F +5 more
europepmc +3 more sources
The Identification and Characterization of a Novel Alginate Lyase from <i>Mesonia hitae</i> R32 Exhibiting High Thermal Stability and Potent Antioxidant Oligosaccharide Production. [PDF]
Ye Y, Li Z, Zhou Y, Gao X, Yan D.
europepmc +3 more sources

