Results 61 to 70 of about 130,472 (328)

FimH as a scaffold for regulated molecular recognition

Journal of Biological Engineering, 2021
Background Recognition proteins are critical in many biotechnology applications and would be even more useful if their binding could be regulated. The current gold standard for recognition molecules, antibodies, lacks convenient regulation.
Shivani Gupta Ludwig, Casey L. Kiyohara, Laura A. Carlucci, Dagmara Kisiela, Evgeni V. Sokurenko, Wendy Evelyn Thomas   +5 more
doaj   +1 more source

Modeling effects of L-type ca(2+) current and na(+)-ca(2+) exchanger on ca(2+) trigger flux in rabbit myocytes with realistic T-tubule geometries. [PDF]

, 2012
The transverse tubular system of rabbit ventricular myocytes consists of cell membrane invaginations (t-tubules) that are essential for efficient cardiac excitation-contraction coupling. In this study, we investigate how t-tubule micro-anatomy, L-type Ca(
Andrew D McCulloch, Anushka Plamenova Michailova, Frank B Sachse, Frank B Sachse, J Andrew eMcCammon, Johan E Hake, Johan E Hake, John H Bridge, Michael J Holst, Peter M Kekenes-Huskey, Yuhui eCheng   +10 more
core   +2 more sources

Integrative residue-intuitive machine learning and MD Approach to Unveil Allosteric Site and Mechanism for β2AR

Nature Communications
Allosteric drugs offer a new avenue for modern drug design. However, the identification of cryptic allosteric sites presents a formidable challenge. Following the allostery nature of residue-driven conformation transition, we propose a state-of-the-art ...
Xin Chen, Kexin Wang, Jianfang Chen, Chao Wu, Jun Mao, Yuanpeng Song, Yijing Liu, Zhenhua Shao, Xuemei Pu   +8 more
doaj   +1 more source

Mapping allosteric pathway in NIa‐Pro using computational approach

Quantitative Biology, 2023
Background Computer simulation studies complement in vitro experiments and provide avenue to understand allosteric regulation in the absence of other molecular viewing techniques. Molecular dynamics captures internal motion within the protein and enables
Rashmi Panigrahi, Senthilkumar Kailasam
doaj   +1 more source

Protein dynamics underlying allosteric regulation

Current Opinion in Structural Biology
Allostery is the mechanism by which information and control are propagated in biomolecules. It regulates ligand binding, chemical reactions, and conformational changes. An increasing level of experimental resolution and control over allosteric mechanisms promises a deeper understanding of the molecular basis for life and powerful new therapeutics.
Miro A. Astore, Akshada S. Pradhan, Erik H. Thiede, Sonya M. Hanson   +3 more
openaire   +2 more sources

Integration of circadian and hypoxia signaling via non‐canonical heterodimerization

FEBS Letters, EarlyView.
CLOCK, BMAL1, and HIFs are basic helix‐loop‐helix and Per‐Arnt‐Sim domain (bHLH‐PAS) proteins, which function as transcription factors. bHLH‐PAS proteins are designated in two classes. Many class I proteins are regulated by environmental signals via their PAS domains, but such signals have not been identified for all.
Sicong Wang, Katja A. Lamia
wiley   +1 more source

Thermodynamic and Kinetic Analysis of Sensitivity Amplification in Biological Signal Transduction [PDF]

, 2002
Based on a thermodynamic analysis of the kinetic model for the protein phosphorylation-dephosphorylation cycle, we study the ATP (or GTP) energy utilization of this ubiquitous biological signal transduction process.
Bourne, Ferrell, Goldbeter, Goldbeter, Gresser, Hartwell, Hill, Hill, Hopfield, Hopfield, Huang, Koshland, Koshland, Krebs, Kushmerick, Li, McKeithan, Poland, Qian, Qian, Shacter, Stadtman, Stryer, Wyman   +23 more
core   +5 more sources

Design of an allosterically regulated retroaldolase [PDF]

Protein Science, 2015
AbstractWe employed a minimalist approach for design of an allosterically controlled retroaldolase. Introduction of a single lysine residue into the nonenzymatic protein calmodulin led to a 15,000‐fold increase in the second order rate constant for retroaldol reaction with methodol as a substrate.
Elizabeth A, Raymond, Korrie L, Mack, Jennifer H, Yoon, Olesia V, Moroz, Yurii S, Moroz, Ivan V, Korendovych   +5 more
openaire   +2 more sources

Conserved structural motifs in PAS, LOV, and CRY proteins regulate circadian rhythms and are therapeutic targets

FEBS Letters, EarlyView.
Cryptochrome and PAS/LOV proteins play intricate roles in circadian clocks where they act as both sensors and mediators of protein–protein interactions. Their ubiquitous presence in signaling networks has positioned them as targets for small‐molecule therapeutics. This review provides a structural introduction to these protein families.
Eric D. Brinckman, Anna E. Lester, Brian D. Zoltowski   +2 more
wiley   +1 more source

Doing justice to allosteric regulation

Comptes Rendus. Biologies, 2015
Jacques Monod gave us not only our first regulatory system, but also our first smart molecules – i.e., he gave us allosteric proteins. But both of these contributions hung in a certain tension with his primary commitments. In particular, I focus here on the ways in which his ontological commitments constrained his thinking about ...
openaire   +2 more sources