Results 161 to 170 of about 1,411 (182)

Advances in the biosynthesis of D-allulose

World Journal of Microbiology and Biotechnology
D-allulose is a rare monosaccharide and a C-3 epimer of D-fructose. It has physiological functions, such as antihyperglycemic, obesity-preventing, neuroprotective, and reactive oxygen species (ROS) scavenging effects, making it an ideal sugar substitute. The synthesis methods for D-allulose include chemical synthesis and biosynthesis.
Yue, Zhang, Zhengsong, Zhou, Haoni, Luan, Xue, Zhang, Mengyu, Liu, Kuiming, Wang, Fei, Wang, Wei, Feng, Wei, Xu, Peng, Song   +9 more
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D-allulose, a versatile rare sugar: recent biotechnological advances and challenges

Critical Reviews in Food Science and Nutrition, 2021
D-Allulose is the C-3 epimer of D-fructose, and widely regarded as a promising substitute for sucrose. It's an excellent low-calorie sweetener, with 70% sweetness of sucrose, 0.4 kcal/g dietary energy, and special physiological functions. It has been approved as GRAS by the U.S. Food and Drug Administration, and is allowed to be excluded from total and
Wenli Zhang, Ding Chen, Jiajun Chen, Wei Xu, Qiuming Chen, Hao Wu, Cuie Guang, Wanmeng Mu   +7 more
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Insulin mimetic effect of D‐allulose on apolipoprotein A‐I gene

Journal of Food Biochemistry, 2022
Several nutrients modulate the transcriptional activity of the apolipoprotein A-I (apo A-I) gene. To determine the influence of rare sugars on apo A-I expression in hepatic (HepG2) and intestinal derived (Caco-2) cell lines, apo A-I, albumin, and SP1 were quantified with enzyme immunoassay and Western blots while mRNA levels were quantified with real ...
Michael J. Haas, Shrina Parekh, Poonam Kalidas, Angela Richter, Firas Warda, Norman C. W. Wong, Masaaki Tokuda, Arshag D. Mooradian   +7 more
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Engineering Escherichia coli for D-allulose biosynthesis from glycerol

Journal of Biotechnology
D-allulose, a naturally occurring monosaccharide, is present in small quantities in nature. It is considered a valuable low-calorie sweetener due to its low absorption in the digestive tract and zero energy for growth. Most of the recent efforts to produce D-allulose have focused on in vitro enzyme catalysis. However, microbial fermentation is emerging
Qiang, Guo, Zhen-Xing, Dong, Xuan, Luo, Ling-Jie, Zheng, Li-Hai, Fan, Hui-Dong, Zheng   +5 more
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A study of D-allulose-associated reproductive toxicity in rats

Food and Chemical Toxicology, 2019
In this study, we assessed whether D-allulose was associated with reproductive toxicity in rats, assessing reproduction and offspring growth following gavage of parents with 0, 500, 1000, and 2000 mg/kg of this compound. Specifically, female rats were continuously dose from 2 weeks prior to mating until day 21 of lactation, while males were dose for ...
Hyejung, Kim, Chongjin, Park, Soonok, Sa, Iris, Case, Chunmei, Li, Yonglin, Gao, Hongbo, Wang, Jingwei, Tian   +7 more
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Designable immobilization of D-allulose 3-epimerase on bimetallic organic frameworks based on metal ion compatibility for enhanced D-allulose production

International Journal of Biological Macromolecules
D-allulose, a low-calorie rare sugar catalyzed by D-allulose 3-epimerase (DAE), is highly sought after for its potential health benefits. However, poor reusability and stability of DAE limited its popularization in industrial applications. Although metal-organic frameworks (MOFs) offer a promising enzyme platform for enzyme immobilization, developing ...
Huayang, Tang, Yian, Chen, Dexun, Fan, Fengguang, Zhao, Shuangyan, Han   +4 more
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Elucidation of d-allulose recognition mechanism in ketose 3-epimerase

Journal of Bioscience and Bioengineering
d-Allulose is a low-calorie sweetener with multiple nutritional functions that can be produced through d-fructose isomerization by ketose 3-epimerase (KEase). l-Ribulose 3-epimerase from Arthrobacterglobiformis (AgLRE) is one of the most important enzymes that produce d-allulose; however, its substrate recognition mechanism is unknown.
Masahiro Watanabe, Yusuke Nakamichi, Shohei Mine   +2 more
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Identification of hyperthermophilic D-allulose 3-epimerase from Thermotoga sp. and its application as a high-performance biocatalyst for D-allulose synthesis

Bioprocess and Biosystems Engineering
D-Allulose 3-epimerase (DAE) is a vital biocatalyst for the industrial synthesis of D-allulose, an ultra-low calorie rare sugar. However, limited thermostability of DAEs hinders their use at high-temperature production. In this research, hyperthermophilic TI-DAE (Tm = 98.4 ± 0.7 ℃) from Thermotoga sp. was identified via in silico screening.
Ji-Dong, Shen, Bao-Ping, Xu, Te-Li, Yu, Yong-Xiang, Fei, Xue, Cai, Liang-Gang, Huang, Li-Qun, Jin, Zhi-Qiang, Liu, Yu-Guo, Zheng   +8 more
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Semi‐rational engineering of D‐allulose 3‐epimerase for simultaneously improving the catalytic activity and thermostability based on D‐allulose biosensor

Biotechnology Journal
AbstractBackgroundD‐Allulose is one of the most well‐known rare sugars widely used in food, cosmetics, and pharmaceutical industries. The most popular method for D‐allulose production is the conversion from D‐fructose catalyzed by D‐allulose 3‐epimerase (DAEase).
Zijie Li, Yangfan Hu, Cheng Yu, Kangqing Fei, Liqun Shen, Yishi Liu, Hideki Nakanishi   +6 more
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Computer-Aided Multidimensional Strategy To Improve the Thermostability of d-Allulose 3-Epimerase from Christensenellaceae Bacterium for the Production of d-Allulose

Journal of Agricultural and Food Chemistry
d-Allulose, a rare sugar with broad applications, is produced from d-fructose by d-allulose 3-epimerase (DAE). However, a high temperature is needed for producing d-allulose, and it is always a challenge to improve both the thermostability and catalytic efficiency of DAEs.
Lin Zheng, Xiao-Jing Zhou, Tian-Yue Jin, Jun-Long Ma, Jing-Wei Zhang, Xue Cai, Zhi-Qiang Liu, Li-Qun Jin, Yu-Guo Zheng   +8 more
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