Results 61 to 70 of about 3,104,746 (304)

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Retinoic acid regulates the ubiquitin–proteasome system in a middle cerebral artery occlusion animal model

Laboratory Animal Research, 2022
Background Retinoic acid is a major metabolite of vitamin A and exerts beneficial effects including anti-oxidant and anti-inflammatory activities in neurons.
Ju-Bin Kang, Murad-Ali Shah, Dong-Ju Park, Phil-Ok Koh   +3 more
doaj   +1 more source

Architecture of coatomer: Molecular characterization of delta-COP and protein interactions within the complex [PDF]

, 1996
Copyright © 2011 by The Rockefeller University Press.Coatomer is a cytosolic protein complex that forms the coat of COP I-coated transport vesicles.
Faulstich, D, S Wegchingel, S Auerbach, L Orci, F T Wieland, Wegehingel, S, Ravazzola, M, Auerbach, S, Harter, C, H Tschochner, Stenbeck, G, Lottspeich, F, Wegchingel, S, F Lottspeich, Orci, L, G Stenbeck, Tschochner, H, D Faulstich, C Harter, M Ravazzola, Wieland, FT   +20 more
core   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source

Ga and Gß Proteins Regulate the Cyclic AMP Pathway That Is Required for Development and Pathogenicity of the Phytopathogen Mycosphaerella graminicola [PDF]

, 2009
We identified and functionally characterized genes encoding three G alpha proteins and one G beta protein in the dimorphic fungal wheat pathogen Mycosphaerella graminicola, which we designated MgGpa1, MgGpa2, MgGpa3, and MgGpb1, respectively.
Lee, T.A.J., van der, Wit, P.J.G.M., de, Kema, G.H.J., M'Barek, S., Ben, Waalwijk, C., Mehrabi, A.   +5 more
core   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

A novel non-lens betagamma-crystallin and trefoil factor complex from amphibian skin and its functional implications. [PDF]

PLoS ONE, 2008
In vertebrates, non-lens betagamma-crystallins are widely expressed in various tissues, but their functions are unknown. The molecular mechanisms of trefoil factors, initiators of mucosal healing and being greatly involved in tumorigenesis, have remained
Shu-Bai Liu, Ying-Ying He, Yun Zhang, Wen-Hui Lee, Jin-Qiao Qian, Ren Lai, Yang Jin   +6 more
doaj   +1 more source

Identification and characterization of a novel splice variant of gonadotropin alpha subunit in the common carp Cyprinus carpio

, 2007
In this study, an alternative splicing transcript GtH-alpha 291 was identified by RT-PCR, which is 291 nt and exists not only in the pituitary but also in the ovary in common carp Cyprinus carpio.
Wang, Y.-P., Hu, W, Chinese Acad Sci, Inst Hydrobiol, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu S Rd, Wuhan 430072, Peoples R China, Hu, W., Liu, W.-Y., Wang, Y., Zhu, Z.-Y.   +5 more
core   +1 more source

(Na+ + K+)-ATPase and plasma membrane polarity of intestinal epithelial cells : presence of a brush border antigen in the distal large intestine that is immunologically related to beta subunit [PDF]

, 1989
The previously produced monoclonal antibody IEC 1/48 against cultured rat intestinal crypt cells (Quaroni, A., and K. J. Isselbacher. 1981. J. Natl. Cancer Inst.
Quaroni, A., B Stieger, Kashgarian, M., Marxer, A., A Marxer, A Quaroni, M Kashgarian, Stieger, B., Hauri, H. P., H P Hauri   +9 more
core   +1 more source

The heterodimeric amino acid transporters (HAT) of the SLC7/SLC3 family: A structure−function relationships and relevance to human pathology

FEBS Letters, EarlyView.
Heterodimeric amino acid transporters consist of SLC7 and SLC3 family proteins arranged in a conserved structural organization. They regulate nutrient transport across cell membranes, supporting essential cellular functions. These transporters also contribute to xenobiotic/drug uptake and distribution.
Mariafrancesca Scalise, Tiziano Mazza, Raffaella Scanga, Lara Console, Michele Galluccio, Cesare Indiveri   +5 more
wiley   +1 more source