Results 201 to 210 of about 12,712 (251)

Choosing between dual and three-drug antiretroviral therapy in aging and comorbid people with HIV: a narrative review. [PDF]

Front Reprod Health
Cossu MV, Matone M, Iannone V, Fusetti C, Colombo V, Gori A, Ridolfo AL, Gervasoni C.   +7 more
europepmc   +1 more source

The Functional Role of S280 within the ZYG-1 Phosphorylation Cluster During Centrosome Assembly in <i>C. elegans</i> Embryos. [PDF]

MicroPubl Biol
Christian A, Richardson S, Obeid L, Shaffou A, DiPanni J, Song MH.   +5 more
europepmc   +1 more source

Efficient and Site-Specific Incorporation of 3-Nitro-Tyrosine Into Recombinant Proteins in <i>Escherichia coli</i>. [PDF]

Bio Protoc
McGee SB, Stanisheuski S, Mehl RA, Cooley RB.   +3 more
europepmc   +1 more source

Azide-functionalized SpCas9 enables generation of site-selective and bioactive Cas9-siRNA conjugates.

Chem Commun (Camb)
Wilbie D, Timmers M, Kogkalidou A, Hebels ER, Sweet IR, Maas-Bakker R, Bosman E, de Jong OG, Vermonden T, Mastrobattista E.   +9 more
europepmc   +1 more source

Modification of nanobodies with 'click' functionalities using the amber suppression technique

, 2015
openaire   +1 more source

Pyrrolysine Amber Stop-Codon Suppression: Development and Applications [PDF]

ChemBioChem, 2017
AbstractThe pyrrolysine tRNA synthetase‐tRNA pair is probably one of the most promiscuous tRNA–synthetase pairs found in nature, capable of genetically encoding a plethora of noncanonical amino acids through stop codon reassignment. Proteins containing reactive handles, post‐translational modification mimics or both can be produced in practical ...
Martin Fascione, Robin Brabham
exaly   +4 more sources

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Generation of Amber Suppression Cell Lines Using CRISPR-Cas9

Methods in Molecular Biology, 2023
Genetic code expansion via amber suppression allows cotranslational, site-specific introduction of nonnatural chemical groups into proteins in the living cell. The archaeal pyrrolysine-tRNA/pyrrolysine-tRNA synthetase (PylT/RS) pair from Methanosarcina mazei (Mma) has been established for incorporation of a wide range of noncanonical amino acids (ncAAs)
Simon J. Elsässer
exaly   +4 more sources

Site-Specific Labelling of Multidomain Proteins by Amber Codon Suppression [PDF]

Scientific Reports, 2018
Abstract Amber codon suppression is a powerful tool to site-specifically modify proteins to generate novel biophysical probes. Yet, its application on large and complex multidomain proteins is challenging, leading to difficulties during structural and conformational characterization using spectroscopic methods.
Christina S Heil, Martin Grininger, Alexander Rittner   +2 more
exaly   +4 more sources

Suppression of an amber mutation by microinjection of suppressor tRNA in C. elegans

Nature, 1982
Informational suppression by nonsense suppressor tRNAs has classically been a powerful tool for study of the mechanism of protein synthesis, to obtain conditional mutants and to demonstrate that a gene encodes a given protein product (for reviews see refs 1–3).
Judith Kimble, John Smith
exaly   +3 more sources