Results 221 to 230 of about 12,712 (251)

Enhancing phage display of antibody fragments using gIII-amber suppression

Gene, 2007
The effect of utilizing Ex12 helper phage, a mutant M13K07 helper having two amber codons at the gIII (gIII-amber), in combination with Escherichia coli host strains belonging to the supE genotype on improving the phage display of antibody fragments was investigated.
Mi-Young, Oh, Hyun-Yoo, Joo, Byung-Ung, Hur, Yeon-Ho, Jeong, Sang-Hoon, Cha   +4 more
openaire   +2 more sources

First position wobble in codon-anticodon pairing: amber suppression by a yeast glutamine tRNA

Gene, 1986
A 2.4-kb fragment of DNA isolated from the Saccharomyces cerevisiae genome was found to suppress amber mutations when its carrier plasmid was present in high copy number. A 1.2-kb subclone of this fragment was sufficient to confer suppressor activity. Sequencing has established that this fragment carries a normal glutamine tRNA gene.
Robert K. Mortimer, R K Mortimer
exaly   +3 more sources

Generation of Stable Amber Suppression Cell Lines

2018
Noncanonical amino acid mutagenesis via amber suppression provides the means to tailor proteins inside living cells. A wide range of noncanonical amino acids have been incorporated using the Methanococcus pyrrolysyl-tRNA synthetase/tRNACUA (PylRS/PylT) in mammalian cell systems in proof of principle experiments, for (1) minimal genetically encoded ...
openaire   +3 more sources

Amber suppression relaxes stringent control by elongating stringent factor

Molecular and General Genetics MGG, 1982
Efficient expression of an amber suppressing tRNA Su+7, relaxes E. coli's stringent response to amino acid starvation. This suppressor tRNA interferes with the accumulation of (p)ppGpp rather than the cell's ability to respond to it, and this appears to be independent of which amino acid is withdrawn. Isogenic UAA- or UGA-reading derivatives of Su+7 do
Linda Breeden, Michael Yarus
openaire   +1 more source

Effect of neighboring nucleotide sequences on suppression efficiency in amber mutants of T4 phage lysozyme

Molecular Genetics and Genomics, 1976
Variations in suppression efficiency were observed among nonsense mutations at different locations within the lysozyme gene (e) of T4 phage. The present experiments using three amber mutants in lysozyme gene indicate such variations presumably depend upon the base sequences neighboring to the nonsense mutations.
Masayori Inouye, Akira Tsugita, A Tsugita   +2 more
exaly   +3 more sources

Gene replacement without selection: regulated suppression of amber mutations in Escherichia coli

Gene, 2003
We have developed a method called "gene gorging" to make precise mutations in the Escherichia coli genome at frequencies high enough (1-15%) to allow direct identification of mutants by PCR or other screen rather than by selection. Gene gorging begins by establishing a donor plasmid carrying the desired mutation in the target cell.
Christopher D, Herring, Jeremy D, Glasner, Frederick R, Blattner   +2 more
openaire   +2 more sources

Amber suppression in the archaebacterium Haloferax volcanii

2009
The purpose of this project was to test whether amber suppression can occur in Haloferax volcanii or not, and if so, to construct a H. volcanii strain that can suppress amber mutations. To achieve this goal, a putative amber suppressor was constructed from the tyrosine transfer RNA of H. volcanii.
openaire   +1 more source

Methionine Regulatory Defects in Salmonella typhimurium Arising from Amber-suppressible Mutations

Journal of General Microbiology, 1972
SUMMARY: Cultures of 15 each newly isolated ethionine-resistant metJ and K regulatory mutants of Salmonella typhimurium were infected with an Escherichia coli F'lac episome with an amber (UAG) nonsense mutation in the lacZ gene. F'Lac+ ethionine-sensitive derivatives segregating Lac- bacteria were obtained from one metJ and one metK mutant.
A C, Minson, D A, Smith
openaire   +2 more sources

Evolved Sequence Contexts for Highly Efficient Amber Suppression with Noncanonical Amino Acids

ACS Chemical Biology, 2014
The expansion of the genetic code with noncanonical amino acids (ncAA) enables the function of proteins to be tailored with high molecular precision. In this approach, the ncAA is charged to an orthogonal nonsense suppressor tRNA by an aminoacyl-tRNA-synthetase (aaRS) and incorporated into the target protein in vivo by suppression of nonsense codons in
Moritz, Pott, Moritz Johannes, Schmidt, Daniel, Summerer   +2 more
openaire   +2 more sources

das Mutation in bacteriophage T4D does not suppress an amber mutation in T4 gene 59

Journal of Virology, 1975
Mutations termed das were isolated originally (Hercules and Wiberg, 1971) as partial suppressors of mutants in phage T4 genes 46 and 47. Since mutants in genes 46, 47, and 59 exhibit both an early arrest of phage DNA synthesis and the loss of this arrest in the presence of chloramphenicol or of mutations of T4 genes 33 and 55, we asked whether a das ...
J S, Wiberg, R S, Swanson
openaire   +2 more sources