Results 211 to 220 of about 12,712 (251)

In vitro synthesis of T4 lysozyme by suppression of amber mutations. [PDF]

Proceedings of the National Academy of Sciences of the United States of America, 1967
W Salser, A Bolle, R F Gesteland
exaly   +3 more sources

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Suppression of a yeast amber mutation in Escherichia coli

Nature, 1979
THE complementation of Escherichia coli auxotrophs by cloned eukaryotic genes1–4 makes it possible to use standard bacterial genetic techniques to study these eukaryotic genes. Here, we describe the cloning of a Saccharomyces cerevisiae (yeast) gene with an amber suppressible allele.
Kevin Struhl, Ronald W. Davis, Gerald Fink   +2 more
exaly   +3 more sources

Molecular consequences of the amber mutation and its suppression

Journal of Molecular Biology, 1965
Each amber mutant of the head protein of bacteriophage T4D produces a characteristic fragment of the polypeptide chain when grown on su − strains of Escherichia coli . On su + strains chain propagation occurs, but chain termination is not completely prevented.
A O, STRETTON, S, BRENNER
exaly   +3 more sources

Ribosomal mutations affecting efficiency of amber suppression

Journal of Molecular Biology, 1970
Abstract The effect of strA mutations on the efficiency of amber and ochre suppressors, known to be mutated tRNA's, has been measured in vivo in isogenic strains. This effect is expressed in terms of translation rate of the amber codon, using bacterial and T4 amber mutants.
P, Strigini, L, Gorini
openaire   +2 more sources

Polarity of amber mutations and suppressed amber mutations in the galactose operon of E. coli

Molecular and General Genetics MGG, 1967
Amber mutants in the t gene of the galactose operon have been examined for polarity in the presence and absence of the suppressors su I and su yMel . In the absence of suppressors there is a gradient of polarity with the more polar mutations nearer the epimerase gene.
E, Jordan, H, Saedler
openaire   +2 more sources

Uridine-33 in yeast tRNA not essential for amber suppression

Nature, 1983
The nucleotide at position 33 on the 5' side of the anticodon of almost all tRNAs is a uridine. Crystallographic studies of different tRNAs reveal that although the precise orientation of uridine-33 is not always the same, it connects the anticodon stacked along the 3' side of the loop with the pyrimidine-32 stacked on the 5' side of the loop.
L, Bare, A G, Bruce, R, Gesteland, O C, Uhlenbeck   +3 more
openaire   +2 more sources

Suppression of amber mutants in vitro induced by low temperature

Journal of Molecular Biology, 1978
Amber mutations are efficiently and specifically suppressed during protein synthesis in vitro in an Su− S-30 extract at 25 °C, but not at 37 °C. Eight different amber mutations in three different genes have been tested, and all are suppressed. The efficiencies of suppression range from 20 to 35%, when protein synthesis is at the Mg2+ concentration ...
J L, Manley, R F, Gesteland
openaire   +2 more sources

Transfer RNA, activating enzymes and amber suppression

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967
info:eu-repo/semantics ...
Bollen, Alex, Herzog, Albert, Burney, A., Marbaix, Gérard, Huez, Georges   +4 more
openaire   +2 more sources

Amber Suppression: a Nucleotide Change in the Anticodon of a Tyrosine Transfer RNA

Nature, 1968
In certain mutants a single base change alters the meaning of a messenger codon in such a way that, instead of spelling out an amino-acid, it spells out chain termination. Mutants in a quite different gene, called a suppression gene, allow the chain-terminating triplet to be read as an amino-acid. Experiments have shown that this is caused by a mutated
H M, Goodman, J, Abelson, A, Landy, S, Brenner, J D, Smith   +4 more
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Intergenic suppression of amber polynucleotide ligase mutation in bacteriophage T4

Virology, 1970
Abstract An intergenic suppressor ( m ) of the amber ligase mutation ( amH 39 x ) was isolated and a number of possible mechanisms of suppression were investigated. The intergenic suppressor does not appear to involve suppression of the amber codon.
V L, Chan, S, Shugar, K, Ebisuzaki
openaire   +2 more sources