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Unnatural Amino Acid Mutagenesis of GPCRs Using Amber Codon Suppression and Bioorthogonal Labeling

2013
To advance dynamic, temporal, and kinetic studies of the G protein-coupled receptor (GPCR) signalosome, new approaches are required to introduce non- or minimally perturbing labels or probes into expressed receptors. We report here a series of methods that are based on unnatural amino acid mutagenesis of GPCRs using amber codon suppression technology ...
Thomas, Huber   +4 more
openaire   +2 more sources

[Mutator effect of suppressed amber-alleles of early genes of bacteriophage T4].

Genetika, 1977
The mutator effect of amber alleles of three early genes (43, 32, 47), which were suppressed by the bacterial suppressor gene, was studied. There are some advantages in using the suppressed amber alleles instead of ts, because in this case definite amino acid substitutions take place in the protein due to certain suppressor gene effect. For example, in
S I, Alikhanian   +2 more
openaire   +1 more source

Suppressible amber mutants of vesicular stomatitis virus, Indiana serotype

Virus Research, 1985
Brian T. White, Duncan J. McGeoch
openaire   +1 more source

Response and Adaptation of Escherichia coli to Suppression of the Amber Stop Codon

ChemBioChem, 2014
Lei Wang, Steven P. Briggs, Demin Zhou
exaly  

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