Results 11 to 20 of about 10,073 (196)

Crystal structure of carbon-nitrogen hydrolase from Helicobacter pylori G27. [PDF]

Acta Crystallogr F Struct Biol Commun
H. pylori carbon–nitrogen hydrolase was produced and crystallized, and its 2.1 Å resolution structure is reported.Carbon–nitrogen hydrolases (CNHs) are members of the diverse nitrilase superfamily of enzymes that facilitate cellular adaptation to environmental stress by metabolizing nitrogen, detoxifying xenobiotics and catabolizing environmentally ...
Srivastava A, Ayanlade JP, Suleiman L, Udell H, Abendroth J, Lorimer DJ, Edwards TE, Staker BL, Zigweid R, Subramanian S, Myler PJ, Chakafana G, Asojo OA.   +12 more
europepmc   +2 more sources

On the Origin of Substrate Specificity of Enzymes from the Amidohydrolase Superfamily. [PDF]

Angew Chem Int Ed Engl
Comprehensive analyses of two enzyme classes from the amidohydrolase superfamily (AHS) revealed that catalysis proceeds either via 1,4 or 1,6 nucleophilic conjugate addition and that this property defines substrate specificity. Moreover, although all substrates and products are entirely achiral, this mechanistic difference results in an inverted ...
Drexler L, F Fürtges T, Rudack T, Sterner R.   +3 more
europepmc   +2 more sources

Comparative Genomic and Transcriptomic Analysis Reveals Why Paenarthrobacter Strains Are Specialists in the Degradation of the Fungicide Iprodione. [PDF]

Microb Biotechnol
Paenarthrobacter are specialists in degrading the fungicide iprodione. We showed that this trait has evolved through gene duplication of ipaH and ddaH genes, being in the core genome of paenarthrobacters, and further genetic optimisation, with different strains being at different evolution stages depending on their prior exposure to iprodione ...
Michelioudakis V, Zafranas A, Myrisiotis C, Makri S, Katsoula A, Campos M, Vasileiadis S, Karpouzas DG.   +7 more
europepmc   +2 more sources

Penicillin Amidohydrolases in Fungal Autolysis [PDF]

Microbiology and Immunology, 1989
AbstractThe production of penicillin G and penicillin V amidohydrolases or acylases (E.C.3.5.1.11) was studied during the autolysis of filamentous fungi in a mineral medium, and in the same medium with phenoxyacetic acid as inducer. In all the studied fungi, enzymes showing penicillin G and penicillin V amidohydrolase activities were found.
C, Alfonso, L, Cribeiro, F, Reyes
openaire   +2 more sources

Phenoxymethylpenicillin amidohydrolases from Penicillium chrysogenum [PDF]

FEBS Letters, 1996
A phenoxymethylpenicillin amidohydrolase which hydrolyses phenoxymethylpenicillin to 6‐aminopenicillanic acid (6‐APA) has been isolated from two species of Penicillium chrysogenum. The amidohydrolase had a molecular mass of approx. 42 kDa. Its activity with benzylpenicillin as substrate was only 1.5% of that with phenoxymethylpenicillin and it was ...
Whiteman, P.A., Abraham, E.P.
openaire   +2 more sources

Isoenzyme N-Acyl-l-Amino Acid Amidohydrolase NA Increases Ochratoxin A Degradation Efficacy of Stenotrophomonas sp. CW117 by Enhancing Amidohydrolase ADH3 Stability

Microbiology Spectrum, 2022
Ochratoxin A (OTA) is a potent mycotoxin mainly produced by toxicogenic strains of Aspergillus spp. and seriously contaminates foods and feedstuffs. OTA detoxification strategies are significant to food safety.
Nan Chen, Qingru Fei, Han Luo, Zemin Fang, Yazhong Xiao, Zhengjun Du, Yu Zhou   +6 more
doaj   +1 more source

Structure of the Ergothioneine‐Biosynthesis Amidohydrolase EgtC [PDF]

ChemBioChem, 2015
AbstractThe ubiquitous sulfur metabolite ergothioneine is biosynthesized by oxidative attachment of a sulfur atom to the imidazole ring of Nα‐trimethylhistidine. Most actinobacteria, including Mycobacterium tuberculosis, use γ‐glutamyl cysteine as a sulfur donor. In subsequent steps the carbon scaffold of γ‐glutamyl cysteine is removed by the glutamine
Vit, Allegra, Mashabela, Gabriel, Blankenfeldt, Wulf, Seebeck, Florian P.   +3 more
openaire   +5 more sources

Generation of an induced pluripotent stem cell line (TRNDi030-A) from a patient with Farber disease carrying a homozygous p. Y36C (c. 107 A>G) mutation in ASAH1

Stem Cell Research, 2021
Farber disease is an ultra-rare lysosomal storage disease. Mutations in the N-acylsphingosine amidohydrolase (ASAH1) gene, which encodes for the enzyme acid ceramidase (ACDase), cause ceramides to accumulate in the body.
Brianna M. Brooks, Charles D. Yeh, Jeanette Beers, Chengyu Liu, Yu-Shan Cheng, Kirill Gorshkov, Jizhong Zou, Wei Zheng, Catherine Z. Chen   +8 more
doaj   +1 more source

The Progress of Ferroptosis of Immune Cells in the Tumor Microenvironment and Its Impact on Tumorigenesis and Development. [PDF]

Immun Inflamm Dis
The interplay between ferroptosis and the tumor immune microenvironment significantly influences the outcome of cancer immunotherapy. Inducing ferroptosis in tumor cells and immunosuppressive cells, while preventing ferroptosis in effector immune cells, emerges as a promising strategy to enhance the efficacy of immunotherapy. This review highlights the
Zhan F, Hu Y, Jiang X, Fang Z.
europepmc   +2 more sources

Heterologous Expression and Characterization of A Novel Ochratoxin A Degrading Enzyme, N-acyl-L-amino Acid Amidohydrolase, from Alcaligenes faecalis

Toxins, 2019
Ochratoxin A (OTA) is a well-known, natural contaminant in foods and feeds because of its toxic effects, such as nephrotoxicity in various animals. Recent studies have revealed that Alcaligenes faecalis could generate enzymes to efficiently degrade OTA ...
Honghai Zhang, Yunpeng Zhang, Tie Yin, Jing Wang, Xiaolin Zhang   +4 more
doaj   +1 more source