Results 31 to 40 of about 5,574 (177)

Probing the Active Site of Class 3 L-Asparaginase by Mutagenesis: Mutations of the Ser-Lys Tandems of ReAV

Biomolecules
The ReAV enzyme from Rhizobium etli, a representative of Class 3 L-asparaginases, is sequentially and structurally different from other known L-asparaginases. This distinctiveness makes ReAV a candidate for novel antileukemic therapies.
Kinga Pokrywka, Marta Grzechowiak, Joanna Sliwiak, Paulina Worsztynowicz, Joanna I. Loch, Milosz Ruszkowski, Miroslaw Gilski, Mariusz Jaskolski   +7 more
doaj   +1 more source

Probing the active site of Class 3 L-asparaginase by mutagenesis. I. Tinkering with the zinc coordination site of ReAV

Frontiers in Chemistry
ReAV, the inducible Class-3 L-asparaginase from the nitrogen-fixing symbiotic bacterium Rhizobium etli, is an interesting candidate for optimizing its enzymatic potential for antileukemic applications.
Kinga Pokrywka, Marta Grzechowiak, Joanna Sliwiak, Paulina Worsztynowicz, Joanna I. Loch, Milosz Ruszkowski, Miroslaw Gilski, Miroslaw Gilski, Mariusz Jaskolski, Mariusz Jaskolski   +9 more
doaj   +1 more source

SKN-1 regulates stress resistance downstream of amino catabolism pathways

iScience, 2022
Summary: The deleterious potential to generate oxidative stress is a fundamental challenge to metabolism. The oxidative stress response transcription factor, SKN-1/NRF2, can sense and respond to changes in metabolic state, although the mechanism and ...
Phillip A. Frankino, Talha F. Siddiqi, Theodore Bolas, Raz Bar-Ziv, Holly K. Gildea, Hanlin Zhang, Ryo Higuchi-Sanabria, Andrew Dillin   +7 more
doaj   +1 more source

Genetic epilepsies with myoclonic seizures: Mechanisms and syndromes

Epilepsia Open, EarlyView.
Abstract Genetic epilepsy with myoclonic seizures encompasses a heterogeneous spectrum of conditions, ranging from benign and self‐limiting forms to severe, progressive disorders. While their causes are diverse, a significant proportion stems from genetic abnormalities.
Antonietta Coppola, Marica Rubino, Antonella Riva, Pasquale Striano   +3 more
wiley   +1 more source

Thiazoline-Specific Amidohydrolase PurAH Is the Gatekeeper of Bottromycin Biosynthesis [PDF]

Journal of the American Chemical Society, 2019
The ribosomally synthesized and post-translationally modified peptide (RiPP) bottromycin A2 possesses potent antimicrobial activity. Its biosynthesis involves the enzymatic formation of a macroamidine, a process previously suggested to require the concerted efforts of a YcaO enzyme (PurCD) and an amidohydrolase (PurAH) in vivo. In vitro, PurCD alone is
Asfandyar Sikandar, Laura Franz, Okke Melse, Iris Antes, Jesko Koehnke   +4 more
openaire   +2 more sources

Biochemical characterization of L-asparaginase isoforms from Rhizobium etli—the boosting effect of zinc

Frontiers in Chemistry
L-Asparaginases, divided into three structural Classes, catalyze the hydrolysis of L-asparagine to L-aspartic acid and ammonia. The members of Class 3, ReAIV and ReAV, encoded in the genome of the nitrogen fixing Rhizobium etli, have the same fold ...
Joanna Sliwiak, Paulina Worsztynowicz, Kinga Pokrywka, Joanna I. Loch, Marta Grzechowiak, Mariusz Jaskolski, Mariusz Jaskolski   +6 more
doaj   +1 more source

Diverse origins of microbial L-asparaginases and their current miscellaneous applications

Archives of Pharmaceutical Sciences Ain Shams University, 2019
L-asparaginase, also known as amidohydrolase, catalyzes the breakdown of asparagine into aspartic acid and ammonia. Due to its ability to inhibit the biosynthesis of protein lymphoblasts, it is used to treat acute lymphoblastic leukemia (ALL).
Nada A. Abdelrazek, Walid Faisal Elkhatib , Marwa M. Raafat, Mohammad Aboulwafa   +3 more
doaj   +1 more source

Implantable electrochemical sensors for continuous blood monitoring

FlexMat, EarlyView.
Implantable electrochemical sensors enable direct, in situ, and continuous blood monitoring, shifting diagnosis from population‐based averages to dynamic, individualized analysis. This review systematically examines the influence of the unique blood properties on sensor design.
Kuangyi Zou, Yuanzhen Wang, Haidong Wang, Ye Zhang   +3 more
wiley   +1 more source

Studies on racemic triglycerides as urease enzyme inhibitors

Bangladesh Journal of Pharmacology
Amidohydrolase enzymes regulate key pathways, and their dysregulation is linked to infection, immune imbalance, and metabolic dysregulation in cancer. Current inhibitors have issues of cost, environmental persistence, and toxicity.
Hamdullah Khadim Sheikh, Tanzila Arshad, Haroon Khan, Uzma Habib, Cindy Juliet Cristancho Ortiz   +4 more
doaj   +1 more source

Mechanisms of metal-dependent non-redox decarboxylases from quantum chemical calculations

Computational and Structural Biotechnology Journal, 2021
Quantum chemical calculations are today an extremely valuable tool for studying enzymatic reaction mechanisms. In this mini-review, we summarize our recent work on several metal-dependent decarboxylases, where we used the so-called cluster approach to ...
Xiang Sheng, Fahmi Himo
doaj   +1 more source