Results 21 to 30 of about 25,591 (179)

A Radiochemical Assay for Aminopeptidase N

Analytical Biochemistry, 1993
We developed an assay for aminopeptidase N (AmN) in which substrate, Arg-Phe-[3H]anilide (24.9 Ci/mmol), can be used at concentrations (1-200 nM) well below Km (12 microM) and at or below enzyme concentration ([E]). Such reaction conditions simulate those in vivo where peptide hormones in picomolar concentrations (> [E]. Arg-Phe-[3H]anilide can be used
J W, Ryan, A Y, Chung, J A, Nearing, F A, Valido, C, Shun-Cun, P, Berryer   +5 more
openaire   +2 more sources

CD13 (human aminopeptidase N) mediates human cytomegalovirus infection [PDF]

Journal of Virology, 1993
Human cytomegalovirus (HCMV) infects cells by a series of processes including attachment, penetration via fusion of the envelope with the plasma membrane, and transport of the viral DNA to the nucleus. The details of the early events of HCMV infection are poorly understood. We have recently reported that CD13, human aminopeptidase N, a metalloprotease,
C, Söderberg, T D, Giugni, J A, Zaia, S, Larsson, J M, Wahlberg, E, Möller   +5 more
openaire   +2 more sources

Gene cloning and sequencing of aminopeptidase N3, a putative receptor for Bacillus thuringiensis insecticidal Cry1Ac toxin in Helicoverpa armigera (Lepidoptera: Noctuidae)

European Journal of Entomology, 2005
A cDNA encoding aminopeptidase N3 was cloned by degenerated PCR and RACE techniques. The full-length of APN3harm is 3486bp. Open reading frame is 3042bp in length, encoding 1014 amino acid residues.
Gui-Rong WANG, Ge-Mei LIANG, Kong-Ming WU, Yu-Yuan GUO   +3 more
doaj   +1 more source

Proteolytic processing of angiotensin-I in human blood plasma. [PDF]

PLoS ONE, 2013
In mammalian species, except humans, N-terminal processing of the precursor peptide angiotensin I (ANG-1-10) into ANG-2-10 or ANG-3-10 was reported. Here we hypothesize that aminopeptidase-generated angiotensins bearing the same C-terminus as ANG-1-10 ...
Diana Hildebrand, Philipp Merkel, Lars Florian Eggers, Hartmut Schlüter   +3 more
doaj   +1 more source

CD13/aminopeptidase N and murine cytomegalovirus infection [PDF]

Virology, 2005
CD13/aminopeptidase N is a membrane-bound metalloproteinase implicated in human cytomegalovirus (HCMV) infection and pathogenesis. Anti-CD13 antibodies can neutralize HCMV infectivity, and HCMV viremia after bone marrow transplantation induces anti-CD13 autoantibodies which correlate with development of chronic graft vs. host disease.
openaire   +2 more sources

An analytical review of vector- and pathogen-based transmission-blocking vaccine for malaria control

Journal of Vector Borne Diseases, 2022
Malaria is a vector borne disease, considered to be one of the most serious public health problems. The present review focused on the blocking of parasite development in mosquito vectors; one broad strategy for achieving this is Transmission Blocking ...
Renu Jakhar, Neelam Sehrawat, S K Gakhar
doaj   +1 more source

Variable O-glycosylation of CD13 (aminopeptidase N)

Journal of Biological Chemistry, 1991
The monoclonal antibody AG6 was raised against HUVE cells and was confirmed as recognizing CD13 (aminopeptidase N) by its reactivity with the CD13 cDNA transfectant pipz "4". However, in sequential immunoprecipitation studies with another anti-CD13 monoclonal, WM15, neither was able to completely remove the molecules recognized by the other.
P J, O'Connell, V, Gerkis, A J, d'Apice
openaire   +2 more sources

Comparison of the Effects of Native and Commercial Probiotic on Gluten Degradation

Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, 2021
Background and Objectives: The main treatment for celiac disease is a gluten-free diet. Gluten infection occurs in 55%-32% of these patients, which can cause symptoms in these patients.
Elham Rostami, Reza Heidari, ‪Mahdieh Shirzad, Elahe Motevaseli, Mohammad Ali Mazlumi   +4 more
doaj  

Isolation of the Binding Protein of Periplocoside E from BBMVs in Midgut of the Oriental Amyworm Mythimna separata Walker (Lepidoptera: Noctuidae) through Affinity Chromatography

Toxins, 2016
Periplocosides, which are insecticidal compounds isolated from the root bark of Periploca sepium Bunge, can affect the digestive system of insects. However, the mechanism though which periplocosides induces a series of symptoms remains unknown.
Mingxing Feng, Zhenyu He, Yuanyuan Wang, Xiufang Yan, Jiwen Zhang, Zhaonong Hu, Wenjun Wu   +6 more
doaj   +1 more source

Characterization of aminopeptidase N from Torpedo marmorata kidney [PDF]

Biology of the Cell, 1994
Summary— A major antigen of the brush border membrane of Torpedo marmorata kidney was identified and purified by immunoprecipitation. The sequence of its 18 N terminal amino acids was determined and found to be very similar to that of mammalian aminopeptidase N (EC 3.4.11.2).
O'Callaghan, Barbara, Synguelakis, Monique, La Salle, Gildas Le Gal, Morel, Nicolas   +3 more
openaire   +2 more sources