Results 121 to 130 of about 232,331 (307)

Constrained Forest Methane Sink by Thermal Adaptation of Soil Methanotrophs

Advanced Science, EarlyView.
Climate warming can stimulate methanotroph‐mediated CH4 oxidation in forest soils, thereby mitigating the increase in atmospheric CH4 concentrations. By integrating evidence from a macro‐climatic thermal gradient and a 70‐day incubation experiment, this study demonstrates that microbial methanotrophs can adapt to long‐term temperature changes at the ...
Baizhi Jiang, Xi Zhang, Zhenghu Zhou, Nianpeng He, Xuhui Zhou, Hongyang Chen   +5 more
wiley   +1 more source

Insight into endophytic microbial diversity in two halophytes and plant beneficial attributes of Bacillus swezeyi

Frontiers in Microbiology
This study utilized high-throughput sequencing to investigate endophytic bacteria diversity in halophytic plants Anabasis truncate (AT) and Anabasis eriopoda (AE) from the Aral Sea region.
Lei Gao, Lei Gao, Jin-Biao Ma, Jin-Biao Ma, Yin Huang, Yin Huang, Murad Muhammad, Murad Muhammad, Hai-Ting Lian, Hai-Ting Lian, Vyacheslav Shurigin, Vyacheslav Shurigin, Dilfuza Egamberdieva, Dilfuza Egamberdieva, Wen-Jun Li, Wen-Jun Li, Li Li, Li Li   +17 more
doaj   +1 more source

Processing times and steps required for plasmid cloning, purified amplicon, and non-purified amplicon methods.

, 2013
Representative sequencing chromatograms generated from each method are shown. The quality of the raw data obtained from the non-purified amplicon method was comparable with that of the plasmid cloning method.
Debra Han-Lin Kong (416975), Hong Kai Lee (416973), Evelyn Siew-Chuan Koay (416976), Julian Wei-Tze Tang (416974)   +3 more
core   +1 more source

Functional–Taxonomic Scaling Resolves Conflicting Average Genome Size Estimates Across Environmental Gradients

Advanced Science, EarlyView.
Based on genome size‐functional breadth theory, we introduce and validate a framework that adjudicates conflicting average genome size (AGS) estimates by testing which estimate yields a gap between scaled functional diversity and scaled taxonomic diversity that is consistent with the expected positive association between genome size and gene functional
Huanhuan Zhu, Xiaoye Chen, Qiushi Li, Chenyu Sun, Haihua Xia, Yingxin Huang, Cong Wang, Peilin Chen, Cheng Gao   +8 more
wiley   +1 more source

Comparisons between deep amplicon alleles and metagenome data, sequenced genomes, and higher-temperature amplicon data.

, 2018
(A) Distributions of the number of substitutions to the nearest deep read from the high and low-temperature MS metagenome (downward triangle) and OS metagenome (upward triangle); from the two full genomes (circles), and from the high-temperature MS ...
Daniel S. Fisher (3325971), Devaki Bhaya (268447), Michelle Davison (3112482), Michael J. Rosen (178893)   +3 more
core   +1 more source

Supramolecular Zinc–Polyphenol Coordination Assemblies Mitigate Colonic Inflammation by Regulating Mucosal Barrier and Microbiome

Advanced Science, EarlyView.
A carrier‐free zinc‐coordinated polyphenol nanoplatform (ZnCA NPs) is designed as an “all‐in‐one” therapeutic strategy for intestinal inflammation. The ZnCA NPs exhibit enhanced colonic accumulation and broad‐spectrum reactive oxygen and nitrogen species scavenging.
Qiwen Xie, Mai Ye, Xuexia Liu, Jing Wang, Ying Chen, Lu Tan, Jinheng Fu, Xiaochang Huang   +7 more
wiley   +1 more source

Evaluation of DNA Extraction Methods for Microbial Community Profiling in Deadwood Decomposition

MicrobiologyOpen
As technologies advance alongside metabarcoding and metagenomic resources, particularly for larger fungal genomes, DNA extraction methods must be optimized to meet higher thresholds, especially from complex environmental substrates. This study focused on
Yanmei Zhang, Zewei Song, Jonathan S. Schilling   +2 more
doaj   +1 more source

Engineered Transformer Base Editor with Enhanced Editing Efficiency

Advanced Science, EarlyView.
A highly efficient transformer base editor system achieves robust genomic editing in a humanized mouse model. This work establishes a versatile and translatable platform, opening new avenues for precision gene therapy. ABSTRACT Canonical cytosine base editors (CBEs) achieve precise C‐to‐T conversions without inducing DNA double‐strand breaks (DSBs ...
Bowen Chen, Letong Liang, Rui Xu, Tianyi Wang, Hongyan Chen, Daru Lu   +5 more
wiley   +1 more source

PM-profiler: a high-resolution and fast tool for taxonomy annotation of amplicon-based microbiome

Microbiology Spectrum
Amplicon sequencing stands as a cornerstone in microbiome profiling, yet concerns persist regarding its resolution and accuracy. The enhancement of reference databases and annotations marks a new era for 16S rRNA-based profiling.
Haobo Shi, Guosen Hou, Sikai Jiang, Xiaoquan Su   +3 more
doaj   +1 more source

Multifunctional Zinc Glycyrrhizinate‐Integrated Gel for Enhanced Probiotic Delivery and Alleviation of Acute and Spontaneous Colitis

Advanced Science, EarlyView.
Multifunctional oral delivery system—L. reuteri@ZGM—act as both a carrier and therapeutic adjuvant, providing ROS scavenging, and mucin‐interactive properties tailored to the IBD microenvironment. This bioactive gel safeguards L. reuteri from gastric degradation, enhances mucosal adhesion, and promotes long‐term retention at inflamed sites, thereby ...
Mengyun Peng, Xiaochun Chen, Wei Li, Shu Tang, Jun Zhou, Xia Wu, Dan Yuan, Zhongyan Shan, Junfeng Shi   +8 more
wiley   +1 more source