Results 101 to 110 of about 218,037 (308)

Genes, primers and amplicon characteristics.

, 2018
Genes, primers and amplicon characteristics.
Fuchen Yang (5680160), Zheng Liu (28397), Zhongqi Qin (5680154), Xianming Li (2820403), Kexin Cheng (5680151), Junfan Tu (5680157), Hongyan Zhu (329809), Li Yang (6520), Tao Wu (16763)   +8 more
core   +1 more source

Cis‐ and Trans‐Regulatory Factors Independently Shape Phenotypic Heterogeneity of Retinitis Pigmentosa

Advanced Science, EarlyView.
A zebrafish model carrying an identical human RHO S334X allele reveals two independent genetic layers shaping retinitis pigmentosa (RP) severity: a protective 3‐bp cis‐regulatory insertion that attenuates transgene expression, and a dominant trans‐acting modifier that restores a severe phenotype.
Cong Cui, Kotone Nakagawa, Takumi Tateno, Ayaka Dan, Dexin Meng, Yoshihiro Omori, Soma Tomihara, Suzuri Okamoto, Shigeru Sato, Motokazu Tsujikawa   +9 more
wiley   +1 more source

HRM analysis using different amplicon lengths.

, 2016
Multiple amplicon lengths (90, 129, and 212 bp) were tested for mutation analyses in order to optimize the HRM analysis. First row: -d1 curve; second row: -d2 curve. The 90-bp amplicon showed the most interpretable heteroduplex-derived peaks.
Satoshi O. Suzuki (388689), Masahiro Mizoguchi (2967615), Nobuhiro Hata (2967612), Yojiro Akagi (2967624), Koji Iihara (563878), Koji Yoshimoto (73623), Daisuke Kuga (2967621), Ryusuke Hatae (2967618), Hideki Murata (262854)   +8 more
core   +1 more source

UCHL3 Regulates Subgenomic Flaviviral RNA Condensates to Promote Virus Propagation

Advanced Science, EarlyView.
ABSTRACT Flavivirus subgenomic RNAs (sfRNAs) antagonise antiviral defences, yet how sfRNAs are organized and maintained in cells remains poorly understood. Here we identify ubiquitin C‐terminal hydrolase L3 (UCHL3) as a post‐translational regulator of flavivirus sfRNA stability and function.
Oscar Trejo‐Cerro, Anna Beekmayer‐Dhillon, Qi Wen Teo, Lewis Siu, Ming Yuan Li, Kate J. Heesom, Adán Pinto‐Fernández, Sumana Sanyal   +7 more
wiley   +1 more source

A rapid, cost-effective tailed amplicon method for sequencing SARS-CoV-2

BMC Genomics, 2020
Background The global COVID-19 pandemic has led to an urgent need for scalable methods for clinical diagnostics and viral tracking. Next generation sequencing technologies have enabled large-scale genomic surveillance of SARS-CoV-2 as thousands of ...
Daryl M. Gohl, John Garbe, Patrick Grady, Jerry Daniel, Ray H. B. Watson, Benjamin Auch, Andrew Nelson, Sophia Yohe, Kenneth B. Beckman   +8 more
doaj   +1 more source

Deep-amplicon sequencing for monitoring impacted waterways in rural Manitoba

Aquatic environments across Canada constantly receive anthropogenic discharge. In this context, water is considered the main vehicle for the dissemination of pathogens into the environment.
Zambrano-Alvarado, Jocelyn
core   +1 more source

A Chirality‐Converted Bacteriolytic Dodecapeptide Regulates Vibrio‐Induced Polymicrobial Infection and Ameliorates Invasion‐Associated Gut Microbiota Disequilibrium

Advanced Science, EarlyView.
Multidrug‐resistant Vibrio infections are rising rapidly and threaten coastal populations worldwide. This study introduces D‐zp37, a chirality‐engineered antimicrobial peptide with exceptional potency against resistant Vibrio species. D‐zp37 kills planktonic cells, blocks mixed‐species biofilms, disrupts essential bacterial stress responses, and shows ...
Ping Zeng, Qipeng Cheng, Xiaoxu Zhang, Honglan Wang, Jinghan Zhang, Xinyi Ding, Pengfei Zhang, Lanhua Yi, Kwok‐Yin Wong, Kin‐Fai Chan, Sheng Chen, Sharon Shui Yee Leung   +11 more
wiley   +1 more source

Diverse banana endophytes reveal potential genotype-driven community structure affected by domestication

Frontiers in Microbiology
Plant endophytic microbiomes play critical roles in plant health, productivity, and stress tolerance, however, their relationship with host genotype remains poorly understood.
Shiva A. Aghdam, Amanda M. V. Brown
doaj   +1 more source

Effect of HFE gene polymorphism on sustained virological response in patients with chronic hepatitis C and elevated serum ferritin

Arquivos de Gastroenterologia, 2012
CONTEXT: Abnormal serum ferritin levels are found in approximately 20%-30% of the patients with chronic hepatitis C and are associated with a lower response rate to interferon therapy. OBJECTIVE: To determine if the presence of HFE gene mutations had any
Silvia Coelho-Borges, Hugo Cheinquer, Fernando Herz Wolff, Nelson Cheinquer, Luciano Krug, Patricia Ashton-Prolla   +5 more
doaj   +1 more source

Processing times and steps required for plasmid cloning, purified amplicon, and non-purified amplicon methods.

, 2013
Representative sequencing chromatograms generated from each method are shown. The quality of the raw data obtained from the non-purified amplicon method was comparable with that of the plasmid cloning method.
Debra Han-Lin Kong (416975), Hong Kai Lee (416973), Evelyn Siew-Chuan Koay (416976), Julian Wei-Tze Tang (416974)   +3 more
core   +1 more source