Results 61 to 70 of about 232,331 (307)

Breaking the Standard: Can Oxford Nanopore Technologies Sequencing Compete With Illumina in Protistan Amplicon Studies?

Environmental DNA
Advancements in sequencing technologies have revolutionized environmental research, leading to a superior understanding of ecosystem functions. In recent years, high‐throughput sequencing techniques evolved from short‐read Illumina sequencing to long ...
Dana Bludau, Guido Sieber, Manan Shah, Aman Deep, Jens Boenigk, Daniela Beisser   +5 more
doaj   +1 more source

Spatial heterogeneity of hotspot mutations and clonal relatedness in ovarian endometrioid carcinoma [PDF]

Mol Oncol
Spatially resolved analysis revealed heterogeneous distributions of selected cancer‐associated mutations within ovarian endometrioid carcinomas. Shared mutations between paired endometrial and ovarian tumors supported clonal relatedness. These findings highlight the value of sampling multiple tumor regions when interpreting molecular profiles ...
Ashihara T, Murakami K, Takamatsu S, Kanto A, Mori S, Otani T, Sakai K, Kotani Y, Nishio K, Abiko K, Matsumura N.   +10 more
europepmc   +2 more sources

Amplicon sequence bioinformatic analyses

, 2021
Linux shell scripts documenting the full bioinformatic pathway used to analyse amplicon sequence data for pmoA, amoA, hzo, nirS, and mcrA genes.
Dave Clark (10711899)
core   +1 more source

A Hybrid Bioprinting–Microfabrication Approach to Controllable Multitype Spheroid Arrays for Modeling Tumor Heterogeneity

Advanced Healthcare Materials, EarlyView.
This work introduces a “cancer‐in‐a‐well” technology that recreates tumor heterogeneity in vitro using multitype spheroid arrays. Combining bioprinting/microdispensing with microstructured culture devices, the system improves drug screening and modeling of tumor complexity.
Simone Bonetti, Eva Clemente, Emanuela Mensà, Marco de Maria, Fulvia Farabegoli, Emanuela Saracino, Barbara Bortolani, Emanuela Marcelli, Renato Brandimarti, Dario de Biase, Giampaolo Zuccheri   +10 more
wiley   +1 more source

Iterative Selection of DNA Nanostructures for Cellular Uptake

Advanced Materials, EarlyView.
DNA nanostructures are promising cell targeting delivery vehicles for therapeutics, but the targeting behavior is not fully understood. In this study, libraries of DNA nanostructures that can be amplified and sequenced are combined with cellular uptake as a selection pressure to iteratively refine DNA structures taken up in cells to better understand ...
Anjali Rajwar, Lisa Eichhorn, Jakub Palacka, Sandra Ly, Erik Benson   +4 more
wiley   +1 more source

Soil Inorganic Electron Acceptors and Carbon Substrates Hierarchically Govern Wetland Methanogenesis Temperature Response

Advanced Science, EarlyView.
ABSTRACT Wetlands constitute Earth's largest natural methane (CH4) source, yet projections of their climate feedback remain uncertain due to poorly constrained temperature sensitivity (Q10‐CH4) of microbial methanogenesis. While water table dynamics have been demonstrated to regulate wetland methanogenesis by altering key soil conditions such as ...
Shuying Qiu, Qingfang Jing, Xuhui Zhou, Xiaoxin Sun, Zhenyu Wei, Baizhi Jiang, Hongyang Chen   +6 more
wiley   +1 more source

Amplicon sequencing reveals bacterial diversity of indoor air microbiome in hospital buildings [PDF]

Airborne transmission of pathogens in hospitals poses a significant risk for healthcare-associated infections. Traditionally, assessing microorganisms in hospital air relies on culture methods, limiting the identification process.
Shamrulazhar, Shamzir Kamal, Ummu Afeera, Zainul Abid, Hajar Fauzan, Ahmad, Nor Husna, Mat Hussin, Mohd Norhafsam, Maghpor, Junaidah, Zakaria   +5 more
core   +3 more sources

Taxonomic assessment of rumen microbiota using total RNA and targeted amplicon sequencing approaches

, 2016
Taxonomic characterization of active gastrointestinal microbiota is essential to detect shifts in microbial communities and functions under various conditions.
Sun, Xu, Cox, Faith, Guan, Leluo, Li, Fuyong, Janssen, Peter H., Henderson, Gemma   +5 more
core   +1 more source

Attomolar Detection of HIV‐1 With Label‐Free RCA‐rCRISPR on Smartphone

Advanced Science, EarlyView.
A new diagnostic strategy integrating rolling circle amplification (RCA) with a plasmid reporter–based ratiometric CRISPR (rCRISPR) system was developed to detect unfragmented HIV genomes with single‐digit attomolar sensitivity. The method quantifies targets using the plasmid relaxation ratio and employs a smartphone‐based mini‐gel device to enable ...
Noor Mohammad, Anastasiia Steksova, Yuyang Tang, Leyao Huang, Alireza Velayati, Shengwei Zhang, Aditi Dey Poonam, Sina Jamalzadegan, Matthew Breen, Hongjie Chen, Mahsa Bagi, Guochun Jiang, Qingshan Wei   +12 more
wiley   +1 more source

Lactiplantibacillus Plantarum GUANKE Alleviates Allergic Asthma by Restricting Monocyte‐to‐DC Differentiation Via Microbiota‐Associated IAA

Advanced Science, EarlyView.
Lactiplantibacillus plantarum GUANKE remodels the indigenous gut microbiota and is associated with microbiota‐dependent restoration of circulating IAA. IAA treatment attenuates JAK3‐STAT3 and Jag1‐Notch4‐Hey1 signaling and reduces CX3CR1+Ly6C− monocytic precursor generation and pulmonary CD11b+ DC accumulation, together with reduced Th2 inflammation ...
Yujia He, Jielan Mi, Zhiping Zhao, Xiao Zhang, Zhihan Yang, Kun Yue, Yuanming Huang, Liqiong Song, Xiaoting Wen, Jianguo Xu, Zhihong Ren   +10 more
wiley   +1 more source