Results 91 to 100 of about 860,088 (184)

A Sequel to Sanger: Amplicon Sequencing That Scales

, 2017
Although high-throughput sequencers (HTS) have largely displaced their Sanger counterparts, the short read lengths and high error rates of most platforms constrain their utility for amplicon sequencing.
Sujeevan Ratnasingham, Winnie Hallwachs, Thomas WA Braukmann, Suresh Naik, Paul DN Hebert, Daniel H Janzen, Evgeny V Zakharov, Sean WJ Prosser, Jayme E Sones, Jeremy R deWaard, Natalia V Ivanova   +10 more
core   +1 more source

Comparative diagnostic performance of plasma microbial cell-free DNA sequencing versus amplicon-based blood next-generation sequencing in suspected bloodstream or endovascular infection: a single-center study

Antimicrobial Stewardship & Healthcare Epidemiology
We compared plasma microbial cell-free DNA sequencing (mcfDNAseq) with an amplicon-based blood next-generation sequencing (NGS) assay for 10 patients with suspected bloodstream or endovascular infection.
Mayar Al Mohajer, Todd Lasco
doaj   +1 more source

16S rDNA amplicon sequencing raw data

16S rDNA amplicon sequencing machine raw ...
Zhang, Liang
core   +1 more source

Schema of the sample preparation for deep amplicon sequencing.

, 2016
Schema of the sample preparation for deep amplicon sequencing.
Jana Raddová (739305), Shunli Wang (399026), Tomáš Nečas (3280845), Aleš Eichmeier (3280842), Tamas Kovacs (386881), Jana Čechová (739304), Xingguo Ye (487691), Miroslav Baránek (739303)   +7 more
core   +1 more source

Different amplicon targets for sequencing-based studies of fungal diversity

, 2017
Target-gene amplicon sequencing is the most exploited high-throughput sequencing application in microbial ecology. The targets are taxonomically relevant genes with the 16S rRNA being the gold standard for bacteria.
Francesca De Filippis, LAIOLA, MANOLO, DE FILIPPIS, FRANCESCA, Danilo Ercolini, Ercolini, Danilo, BLAIOTTA, GIUSEPPE, Giuseppe Blaiotta, Manolo Laiola   +7 more
core   +1 more source

Evaluation of the reproducibility of amplicon sequencing with Illumina MiSeq platform

PLOS ONE, 2017
Illumina's MiSeq has become the dominant platform for gene amplicon sequencing in microbial ecology studies; however, various technical concerns, such as reproducibility, still exist. To assess reproducibility, 16S rRNA gene amplicons from 18 soil samples of a reciprocal transplantation experiment were sequenced on an Illumina MiSeq.
Chongqing Wen, Liyou Wu, Yujia Qin, Joy D. Van Nostrand, Daliang Ning, Bo Sun, Kai Xue, Feifei Liu, Ye Deng, Yuting Liang, Jizhong Zhou   +10 more
openaire   +6 more sources

Decoding fungal communities: ITS extraction matters more than ASVs vs. OTUs [PDF]

Web Ecology
To capture the complexity of fungal communities, microbial ecologists must often resort to amplicon sequencing. Amplicon sequences are obtained from the nuclear ribosomal internal transcribed spacer (ITS), which is currently used as a marker for fungal ...
G. Tosadori, J. Bosch
doaj   +1 more source

EasyAmplicon 2: Expanding PacBio and Nanopore Long Amplicon Sequencing Analysis Pipeline for Microbiome

Advanced Science
In the past decade, third‐generation sequencing technologies (such as PacBio (Pacific Biosciences) and Nanopore) have become gradually matured and are widely used for microbial taxonomy and quantification.
Hao Luo, Defeng Bai, Zhihao Zhu, Salsabeel Yousuf, Haifei Yang, Jiani Xun, Meiyin Zeng, Yao Wang, Yunyun Gao, Kai Peng, Shanshan Xu, Yuanping Zhou, Tianyuan Zhang, Chuang Ma, Huiyu Hou, Xiulin Wan, Yang Zhou, Baolei Jia, Shi Huang, Renyou Gan, Tao Wen, Tong Chen, Xia Chen, Xiaofang Li, Yong‐Xin Liu   +24 more
doaj   +1 more source

Long-read metagenomics gives a more accurate insight into the microbiota of long-ripened gouda cheeses

Frontiers in Microbiology
Metagenomic studies of the Gouda cheese microbiota and starter cultures are scarce. During the present study, short-read metagenomic sequencing (Illumina) was applied on 89 Gouda cheese and processed milk samples, which have been investigated before ...
Hannes Decadt, Cristian Díaz-Muñoz, Louise Vermote, Inés Pradal, Luc De Vuyst, Stefan Weckx   +5 more
doaj   +1 more source

Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary

BioTechniques, 2019
Conventional wisdom holds that PCR amplification for sequencing should employ pooled replicate reactions to reduce bias due to jackpot effects and chimera formation.
Clarisse Marotz, Anukriti Sharma, Greg Humphrey, Neil Gottel, Christopher Daum, Jack A Gilbert, Emiley Eloe-Fadrosh, Rob Knight   +7 more
doaj   +1 more source