Results 101 to 110 of about 165,809 (296)

RNA Helicase DDX21 Controls CD4+ T Cell Proliferation and Promotes Inflammatory Bowel Disease via Translational Control

Advanced Science, EarlyView.
ABSTRACT Inflammatory bowel disease (IBD) is characterized by dysregulated T cell responses. RNA helicases, including DExD‐box helicase 21 (DDX21), are pivotal in RNA metabolism, but their role in T cell‐mediated pathology during IBD remains unclear. Here, we demonstrate that DDX21 expression in CD4+ T cells correlates with cell cycle and translation ...
Yujuan Zhang, Chen Kan, Xinhui Yang, Yajuan Hao, Xuemin Cai, Mei Yang, Qianqian Zhang, Zhengting Wang, Ning Jiang, Lei Wang, Hua‐Bing Li, Jing Zhou   +11 more
wiley   +1 more source

Evaluation of the reproducibility of amplicon sequencing with Illumina MiSeq platform

PLOS ONE, 2017
Illumina's MiSeq has become the dominant platform for gene amplicon sequencing in microbial ecology studies; however, various technical concerns, such as reproducibility, still exist. To assess reproducibility, 16S rRNA gene amplicons from 18 soil samples of a reciprocal transplantation experiment were sequenced on an Illumina MiSeq.
Chongqing Wen, Liyou Wu, Yujia Qin, Joy D. Van Nostrand, Daliang Ning, Bo Sun, Kai Xue, Feifei Liu, Ye Deng, Yuting Liang, Jizhong Zhou   +10 more
openaire   +6 more sources

Impact of DNA Sequencing and Analysis Methods on 16S rRNA Gene Bacterial Community Analysis of Dairy Products

mSphere, 2018
DNA sequencing and analysis methods were compared for 16S rRNA V4 PCR amplicon and genomic DNA (gDNA) mock communities encompassing nine bacterial species commonly found in milk and dairy products.
Zhengyao Xue, Mary E. Kable, Maria L. Marco   +2 more
doaj   +1 more source

Enhanced Glycolysis‐Driven Histone H3K18 Lactylation Regulates Epileptogenesis by Modulating the E3 Ubiquitin Ligase COP1

Advanced Science, EarlyView.
Neuronal PKM2‐driven glycolysis generates excess lactate that triggers histone H3K18 lactylation (H3K18la), establishing a pathogenic metabolic‐epigenetic axis in epilepsy. Elevated H3K18la enriches the Cop1 promoter, transcriptionally upregulating the E3 ubiquitin ligase COP1, which subsequently drives proteasomal degradation of GABAARβ2 and impairs ...
Yuan Meng, Yanlin Guo, Liumi Jiang, Jing Luo, Yan Liu, Ziwei Yuan, Pingyang Ke, Ran Duan, Fei Xiao   +8 more
wiley   +1 more source

Data from: A sequel to Sanger: amplicon sequencing that scales

, 2018
Although high-throughput sequencers (HTS) have largely displaced their Sanger counterparts, the short read lengths and high error rates of most platforms constrain their utility for amplicon sequencing.
Janzen, Daniel H., Prosser, Sean W.J., Sones, Jayme E., deWaard, Jeremy R., Naik, Suresh, Ratnasingham, Sujeevan, Braukmann, Thomas W.A., Ivanova, Natalia V., Hallwachs, Winnie, Zakharov, Evgeny V., Hebert, Paul D.N.   +10 more
core   +1 more source

Multi‐Enzyme Mimetic Molybdenum Nitride Nanozymes Reshape Subgingival Microenvironment for Synergistic Periodontitis Therapy via ROS Regulation and Microbiome Remodeling

Advanced Science, EarlyView.
ABSTRACT Periodontitis, a chronic inflammatory disease initiated and sustained by plaque microorganisms and host immune response, remains an intractable oral disease and a leading cause of tooth loss worldwide. Traditional mechanical debridement and adjunctive antibiotic or antiseptic therapy often shows limited efficacy due to the complex anatomical ...
Weiyu Zhang, Zhongwei Yang, Yunfan Zhang, Longwei Wang, Xiaoyu Zhang, Jiahui Mao, Yizhi Dai, Yifan Yuan, Mingge Wang, Xin Yang, Xin Yu, Jing Liu, Chunying Chen   +12 more
wiley   +1 more source

Bioinformatic Processing of Amplicon Sequencing Datasets

, 2017
Amplicon sequencing can be a very powerful approach for detecting toxic cyanobacteria or any other kind of microorganism during monitoring programs. However, owing to the huge size of next-generation sequencing (NGS) datasets (up to several Gb), there is an obvious need for semi-automatic data processing and statistical analysis, as well as ...
Sweetlove, Maxime, Obbels, Dagmar, Verleyen, Elie, Stelmach Pessi, Igor, Wilmotte, Annick, Vyverman, Wim   +5 more
openaire   +2 more sources

Sample Preparation for Amplicon Sequencing v1

, 2019
Preparation of PCR products for amplicon sequencing
Anna Székely, Máté Vass
openaire   +1 more source

Decoding fungal communities: ITS extraction matters more than ASVs vs. OTUs [PDF]

Web Ecology
To capture the complexity of fungal communities, microbial ecologists must often resort to amplicon sequencing. Amplicon sequences are obtained from the nuclear ribosomal internal transcribed spacer (ITS), which is currently used as a marker for fungal ...
G. Tosadori, J. Bosch
doaj   +1 more source

Deep-amplicon sequencing for monitoring impacted waterways in rural Manitoba

Aquatic environments across Canada constantly receive anthropogenic discharge. In this context, water is considered the main vehicle for the dissemination of pathogens into the environment.
Zambrano-Alvarado, Jocelyn
core   +1 more source