Results 121 to 130 of about 165,809 (296)

Limited added value of fungal ITS amplicon sequencing in the study of bovine abortion [PDF]

, 2018
Bovine mycotic abortion is sporadic and caused by different ubiquitous and opportunistic fungi. Recently, a broad spectrum of bacterial opportunists involved in bovine abortion was revealed by 16S rRNA gene amplicon sequencing.
Rodriguez-Campos, Sabrina, Dettwiler, Martina, Caroline F. Frey, Gilbert Greub, Vidal, Sara, Martina Dettwiler, Jenny Bressan, Sara Vidal, Vincent Perreten, Carlos Abril, Brandt, B.W.; id_orcid, Brandt, B.W., Perreten, Vincent, Vidal, S., Perreten, V., Frey, C.F., Bernd W. Brandt, Greub, Gilbert, Rodriguez-Campos, S., Brandt, Bernd W., Abril, Carlos, Greub, G., Dettwiler, M., Sabrina Rodriguez-Campos, Bressan, J., Abril, C., Bressan, Jenny, Frey, Caroline F.   +27 more
core   +1 more source

MFPD: A Multiple Fungal Pathogen Detection Pipeline Across Diverse Habitats

Advanced Science, EarlyView.
The MFPD pipeline integrates a comprehensive ITS reference database of fungal pathogens, optimized parameters, and algorithms tailored for both full‐length and subregion sequences that balance accuracy and computational efficiency; it enables high‐throughput, species‐level identification from amplicon sequencing data, supporting large‐scale ...
Yi Shen, Xinrun Yang, Jiabao Yu, Yaozhong Zhang, Tianjie Yang, Yang Gao, Xiaofang Wang, Alexandre Jousset, Waseem Raza, Fang‐Jie Zhao, Qirong Shen, Gaofei Jiang, Zhong Wei, Yangchun Xu   +13 more
wiley   +1 more source

Long-read metagenomics gives a more accurate insight into the microbiota of long-ripened gouda cheeses

Frontiers in Microbiology
Metagenomic studies of the Gouda cheese microbiota and starter cultures are scarce. During the present study, short-read metagenomic sequencing (Illumina) was applied on 89 Gouda cheese and processed milk samples, which have been investigated before ...
Hannes Decadt, Cristian Díaz-Muñoz, Louise Vermote, Inés Pradal, Luc De Vuyst, Stefan Weckx   +5 more
doaj   +1 more source

Cigarette Smoke‐Induced Alveolar Macrophage Senescence via GEM/SIRT3‐Mediated Mitochondrial Dysfunction

Advanced Science, EarlyView.
Cigarette smoke accelerates the aging of immune cells in the lung. By combining human single‐cell sequencing, cell culture, and mouse models, the authors show that the protein GEM drives mitochondrial damage and senescence in alveolar macrophages by suppressing SIRT3.
Jin Wang, Meidan Wei, Xiangrong Song, Jiaxin Zhang, Lisha Zhang, Sixian Chen, Yaoyu Hu, Hui Gao, Jianxiang Li   +8 more
wiley   +1 more source

Amplicon sequencing reveals bacterial diversity of indoor air microbiome in hospital buildings [PDF]

Airborne transmission of pathogens in hospitals poses a significant risk for healthcare-associated infections. Traditionally, assessing microorganisms in hospital air relies on culture methods, limiting the identification process.
Shamrulazhar, Shamzir Kamal, Ummu Afeera, Zainul Abid, Hajar Fauzan, Ahmad, Nor Husna, Mat Hussin, Mohd Norhafsam, Maghpor, Junaidah, Zakaria   +5 more
core  

The BnTFL1‐BnJAM3‐BnSWEETs Module Orchestrates Seed Storage Reserve Accumulation in Brassica napus

Advanced Science, EarlyView.
BnaC03.TFL1 and BnaA01.JAM3 form a complex that facilitates the transport of soluble sugars from the seed coat to the embryo by directly activating the expression of BnSWEETs. Furthermore, BnaC03.TFL1 and BnaA01.JAM3 work cooperatively to promote soluble sugar and oil production while suppressing protein deposition.
Jianjun Wang, Zijin Liu, Minshan Jin, Junting Liu, Shihao Wei, Dengmao Yang, Saiqi Yang, Yuan Guo, Mingxun Chen   +8 more
wiley   +1 more source

Amuc_1473 Links Gut Microbes to Skeletal Homeostasis and Counteracts Multifactorial Osteoporosis

Advanced Science, EarlyView.
Amuc_1473, a previously uncharacterized protein enriched in Akkermansia muciniphila‐derived extracellular vesicles, is identified as a gut–bone messenger that promotes osteogenesis and inhibits osteoclastogenesis by engaging transcriptional and translational regulators in bone cells.
Shan‐Shan Rao, Hai‐Jin Zeng, Zun Wang, Chun‐Gu Hong, Yi‐Juan Tan, Yan‐Xin Duan, Jing‐Yao Luo, Ming‐Jie Luo, Yi‐Wei Liu, Xin Wang, Yi Luo, Teng‐Fei Wan, Yong Zhou, Zheng‐Guang Wang, Guo‐Wen Hu, Hao Yin, Xin‐Yue Hu, Zhen‐Xing Wang, Ze‐Hui He, Si‐Yi Cheng, Wei Du, Zhe Guan, Hai‐Li Lang, Hong‐Ji Liu, Jia Cao, Peng Chen, Jiang‐Hua Liu, Hui Xie, Chun‐Yuan Chen   +28 more
wiley   +1 more source

Comparison of a long-read amplicon sequencing approach to short-read amplicons for microbiome analysis

Abstract Most microbiome studies to date rely on sequencing short amplicons of the 16S rRNA gene on Illumina’s platforms. Because of the short read length, sequences often can be identified reliably only to the family or genus levels. Long read sequencing with whole-length 16S rRNA sequencing can improve taxonomic resolution, but often ...
Brandon O’Sullivan, Katherine W. Herbst, Alexander H. Hogan, Michele Maltz-Matyschsyk, Justin D. Radolf, David Lawrence, Michael A. Lynes, Juan C. Salazar, Joerg Graf   +8 more
openaire   +2 more sources

Development and application of a whole genome amplicon sequencing method for infectious salmon anemia virus (ISAV)

Frontiers in Microbiology
Infectious salmon anemia (ISA) is an infectious disease primarily affecting farmed Atlantic salmon, Salmo salar, which is caused by the ISA virus (ISAV). ISAV belongs to the Orthomyxoviridae family. The disease is a serious condition resulting in reduced
Bjørn Spilsberg, Magnus Leithaug, Debes Hammershaimb Christiansen, Maria Marjunardóttir Dahl, Petra Elisabeth Petersen, Karin Lagesen, Eve M. L. Z. Fiskebeck, Torfinn Moldal, Mette Boye   +8 more
doaj   +1 more source

Multi‐Omics Insights Into the Mechanisms of Early Muscle Fiber Difference and Transformation Between Lean‐Type and Chinese Indigenous Pigs

Advanced Science, EarlyView.
Multi‐omics analyses uncover breed‐specific cis‐regulatory landscapes and higher‐order chromatin architectural differences that underlie early postnatal muscle fiber divergence in pigs. A super‐enhancer upstream of PPP3CB recruits MEF2C to activate PPP3CB transcription, while the PPP3CB–MEF2C positive feedback loop promotes oxidative muscle fiber ...
Shuailong Zheng, Hainan Wu, Min Liu, Kunpeng Wu, Shuntao Huang, Zulfiqar Ahmed, Lenox Pius, Mengjin Zhu, Dequan Xu   +8 more
wiley   +1 more source